Biosynthesis and Genetic Encoding of Non-hydrolyzable Phosphoserine into Recombinant Proteins in Escherichia coli

por Zhu, Phillip, Mehl, Ryan A., Cooley, Richard B.
Publicado 2023

Coincidence detection and bi-directional transmembrane signaling control a bacterial second messenger receptor

por Cooley, Richard B, O’Donnell, John P, Sondermann, Holger
Publicado 2016

Concatenation of 14-3-3 with partner phosphoproteins as a tool to study their interaction

por Tugaeva, Kristina V., Kalacheva, Daria I., Cooley, Richard B., Strelkov, Sergei V., Sluchanko, Nikolai N.
Publicado 2019

Accessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditions

por Vesely, Cat Hoang, Reardon, Patrick N., Yu, Zhen, Barbar, Elisar, Mehl, Ryan A., Cooley, Richard B.
Publicado 2022

Mechanistic insight into the conserved allosteric regulation of periplasmic proteolysis by the signaling molecule cyclic-di-GMP

por Chatterjee, Debashree, Cooley, Richard B, Boyd, Chelsea D, Mehl, Ryan A, O'Toole, George A, Sondermann, Holger
Publicado 2014

High‐yield recombinant bacterial expression of (13)C‐, (15)N‐labeled, serine‐16 phosphorylated, murine amelogenin using a modified third generation genetic code expansion protoco...

por Buchko, Garry W., Zhou, Mowei, Vesely, Cat Hoang, Tao, Jinhui, Shaw, Wendy J., Mehl, Ryan A., Cooley, Richard B.
Publicado 2023

Structural Basis of Improved Second-Generation 3-Nitro-tyrosine tRNA Synthetases

por Cooley, Richard B., Feldman, Jessica L., Driggers, Camden M., Bundy, Taylor A., Stokes, Audrey L., Karplus, P. Andrew, Mehl, Ryan A.
Publicado 2014

Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA

por Forsythe, Heather M., Rodriguez Galvan, Joaquin, Yu, Zhen, Pinckney, Seth, Reardon, Patrick, Cooley, Richard B., Zhu, Phillip, Rolland, Amber D., Prell, James S., Barbar, Elisar
Publicado 2021

PermaPhos(Ser): autonomous synthesis of functional, permanently phosphorylated proteins

por Zhu, Phillip, Franklin, Rachel, Vogel, Amber, Stanisheuski, Stanislau, Reardon, Patrick, Sluchanko, Nikolai N., Beckman, Joseph S., Karplus, P. Andrew, Mehl, Ryan A., Cooley, Richard B.
Publicado 2021

Human 14-3-3 Proteins Site-selectively Bind the Mutational Hotspot Region of SARS-CoV-2 Nucleoprotein Modulating its Phosphoregulation

por Tugaeva, Kristina V., Sysoev, Andrey A., Kapitonova, Anna A., Smith, Jake L.R., Zhu, Phillip, Cooley, Richard B., Antson, Alfred A., Sluchanko, Nikolai N.
Publicado 2023

Creating a Selective Nanobody Against 3-Nitrotyrosine Containing Proteins

por Van Fossen, Elise M., Grutzius, Sonia, Ruby, Carl E., Mourich, Dan V., Cebra, Chris, Bracha, Shay, Karplus, P. Andrew, Cooley, Richard B., Mehl, Ryan A.
Publicado 2022

Autonomous Synthesis of Functional, Permanently Phosphorylated Proteins for Defining the Interactome of Monomeric 14-3-3ζ

por Zhu, Phillip, Stanisheuski, Stanislau, Franklin, Rachel, Vogel, Amber, Vesely, Cat Hoang, Reardon, Patrick, Sluchanko, Nikolai N., Beckman, Joseph S., Karplus, P. Andrew, Mehl, Ryan A., Cooley, Richard B.
Publicado 2023

A Conserved Regulatory Circuit Controls Large Adhesins in Vibrio cholerae

por Kitts, Giordan, Giglio, Krista M., Zamorano-Sánchez, David, Park, Jin Hwan, Townsley, Loni, Cooley, Richard B., Wucher, Benjamin R., Klose, Karl E., Nadell, Carey D., Yildiz, Fitnat H., Sondermann, Holger
Publicado 2019

Tuning phenylalanine fluorination to assess aromatic contributions to protein function and stability in cells

por Galles, Grace D., Infield, Daniel T., Clark, Colin J., Hemshorn, Marcus L., Manikandan, Shivani, Fazan, Frederico, Rasouli, Ali, Tajkhorshid, Emad, Galpin, Jason D., Cooley, Richard B., Mehl, Ryan A., Ahern, Christopher A.
Publicado 2023