Simplified Gene Knockout by CRISPR-Cas9-Induced Homologous Recombination

por Dalvie, Neil C., Lorgeree, Timothy, Biedermann, Andrew M., Love, Kerry R., Love, J. Christopher
Publicado 2021

Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

por Dalvie, Neil C., Naranjo, Christopher A., Rodriguez-Aponte, Sergio A., Johnston, Ryan S., Christopher Love, J.
Publicado 2022

Identifying Improved Sites for Heterologous Gene Integration Using ATAC-seq

por Brady, Joseph R., Tan, Melody C., Whittaker, Charles A., Colant, Noelle A., Dalvie, Neil C., Love, Kerry Routenberg, Love, J. Christopher
Publicado 2020

Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain

por Brady, Joseph R., Whittaker, Charles A., Tan, Melody C., Kristensen, D. Lee, Ma, Duanduan, Dalvie, Neil C., Love, Kerry Routenberg, Love, J. Christopher
Publicado 2019

SARS-CoV-2 RBD dimers elicit response comparable to VLPs in mice

por Love, J., Rodriguez-Aponte, Sergio, Tostanoski, Lisa, Dalvie, Neil, Johnston, Ryan, Jacob-Dolan, Catherine, Powers, Olivia, Hachmann, Nicole, Miller, Jessica, Hall, Kevin, Siamatu, Mazuba, Mazurek, Camille, Surve, Nehalee, Barouch, Dan
Publicado 2023

Development of a platform process for the production and purification of single‐domain antibodies

por Crowell, Laura E., Goodwine, Chaz, Holt, Carla S., Rocha, Lucia, Vega, Celina, Rodriguez, Sergio A., Dalvie, Neil C., Tracey, Mary K., Puntel, Mariana, Wigdorovitz, Andrés, Parreño, Viviana, Love, Kerry R., Cramer, Steven M., Love, J. Christopher
Publicado 2021

Mechanism of Thimerosal-Induced Structural Destabilization of a Recombinant Rotavirus P[4] Protein Antigen Formulated as a Multi-Dose Vaccine

por Kaur, Kawaljit, Xiong, Jian, Sawant, Nishant, Agarwal, Sanjeev, Hickey, John M., Holland, David A., Mukhopadhyay, Tarit K., Brady, Joseph R., Dalvie, Neil C., Tracey, Mary Kate, Love, Kerry R., Love, J. Christopher, Weis, David D., Joshi, Sangeeta B., Volkin, David B.
Publicado 2021

Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii

por Dalvie, Neil C., Biedermann, Andrew M., Rodriguez-Aponte, Sergio A., Naranjo, Christopher A., Rao, Harish D., Rajurkar, Meghraj P., Lothe, Rakesh R., Shaligram, Umesh S., Johnston, Ryan S., Crowell, Laura E., Castelino, Seraphin, Tracey, Mary Kate, Whittaker, Charles A., Love, J. Christopher
Publicado 2021

Scalable, methanol‐free manufacturing of the SARS‐CoV‐2 receptor‐binding domain in engineered Komagataella phaffii

por Dalvie, Neil C., Biedermann, Andrew M., Rodriguez‐Aponte, Sergio A., Naranjo, Christopher A., Rao, Harish D., Rajurkar, Meghraj P., Lothe, Rakesh R., Shaligram, Umesh S., Johnston, Ryan S., Crowell, Laura E., Castelino, Seraphin, Tracey, Mary K., Whittaker, Charles A., Love, J. Christopher
Publicado 2021

Antigen-adjuvant interactions, stability, and immunogenicity profiles of a SARS-CoV-2 receptor-binding domain (RBD) antigen formulated with aluminum salt and CpG adjuvants

por Bajoria, Sakshi, Kaur, Kawaljit, Kumru, Ozan S., Van Slyke, Greta, Doering, Jennifer, Novak, Hayley, Rodriguez Aponte, Sergio A., Dalvie, Neil C., Naranjo, Christopher A., Johnston, Ryan S., Silverman, Judith Maxwell, Kleanthous, Harry, Love, J. Christopher, Mantis, Nicholas J., Joshi, Sangeeta B., Volkin, David B.
Publicado 2022

Rapid Developability Assessments to Formulate Recombinant Protein Antigens as Stable, Low-Cost, Multi-Dose Vaccine Candidates: Case-Study With Non-Replicating Rotavirus (NRRV) Vacc...

por Sawant, Nishant, Kaur, Kawaljit, Holland, David A., Hickey, John M., Agarwal, Sanjeev, Brady, Joseph R., Dalvie, Neil C., Tracey, Mary Kate, Velez-Suberbie, M. Lourdes, Morris, Stephen A., Jacob, Shaleem I., Bracewell, Daniel G., Mukhopadhyay, Tarit K., Love, Kerry R., Love, J. Christopher, Joshi, Sangeeta B., Volkin, David B.
Publicado 2021

Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

por Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra D., Rodriguez-Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina, Mukhopadhyay, Tarit K., Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
Publicado 2021

Phosphate-mediated coanchoring of RBD immunogens and molecular adjuvants to alum potentiates humoral immunity against SARS-CoV-2

por Rodrigues, Kristen A., Rodriguez-Aponte, Sergio A., Dalvie, Neil C., Lee, Jeong Hyun, Abraham, Wuhbet, Carnathan, Diane G., Jimenez, Luis E., Ngo, Julia T., Chang, Jason Y. H., Zhang, Zeli, Yu, Jingyou, Chang, Aiquan, Nakao, Catherine, Goodwin, Benjamin, Naranjo, Christopher A., Zhang, Libin, Silva, Murillo, Barouch, Dan H., Silvestri, Guido, Crotty, Shane, Love, J. Christopher, Irvine, Darrell J.
Publicado 2021

RBD-VLP Vaccines Adjuvanted with Alum or SWE Protect K18-hACE2 Mice against SARS-CoV-2 VOC Challenge

por Wong, Ting Y., Russ, Brynnan P., Lee, Katherine S., Miller, Olivia A., Kang, Jason, Cooper, Melissa, Winters, Michael T., Rodriguez-Aponte, Sergio A., Dalvie, Neil C., Johnston, Ryan S., Rader, Nathaniel A., Wong, Zeriel Y., Cyphert, Holly A., Martinez, Ivan, Shaligram, Umesh, Batwal, Saurabh, Lothe, Rakesh, Chandrasekaran, Rahul, Nagar, Gaurav, Rajurkar, Meghraj, Rao, Harish, Bevere, Justin R., Barbier, Mariette, Love, J. Christopher, Damron, F. Heath
Publicado 2022

Intranasal vaccination with lipid-conjugated immunogens promotes antigen transmucosal uptake to drive mucosal and systemic immunity

por Hartwell, Brittany L., Melo, Mariane B., Xiao, Peng, Lemnios, Ashley A., Li, Na, Chang, Jason Y.H., Yu, Jingyou, Gebre, Makda S., Chang, Aiquan, Maiorino, Laura, Carter, Crystal, Moyer, Tyson J., Dalvie, Neil C., Rodriguez-Aponte, Sergio A., Rodrigues, Kristen A., Silva, Murillo, Suh, Heikyung, Adams, Josetta, Fontenot, Jane, Love, J. Christopher, Barouch, Dan H., Villinger, Francois, Ruprecht, Ruth M., Irvine, Darrell J.
Publicado 2022

Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variants

por Rodriguez-Aponte, Sergio A., Dalvie, Neil C., Wong, Ting Y., Johnston, Ryan S., Naranjo, Christopher A., Bajoria, Sakshi, Kumru, Ozan S., Kaur, Kawaljit, Russ, Brynnan P., Lee, Katherine S., Cyphert, Holly A., Barbier, Mariette, Rao, Harish D., Rajurkar, Meghraj P., Lothe, Rakesh R., Shaligram, Umesh S., Batwal, Saurabh, Chandrasekaran, Rahul, Nagar, Gaurav, Kleanthous, Harry, Biswas, Sumi, Bevere, Justin R., Joshi, Sangeeta B., Volkin, David B., Damron, F. Heath, Love, J. Christopher
Publicado 2023

On-demand manufacturing of clinical-quality biopharmaceuticals

por Crowell, Laura E., Lu, Amos E., Love, Kerry R., Stockdale, Alan, Timmick, Steven M., Wu, Di, Wang, Yu (Annie), Doherty, William, Bonnyman, Alexandra, Vecchiarello, Nicholas, Goodwine, Chaz, Bradbury, Lisa, Brady, Joseph R., Clark, John J., Colant, Noelle A., Cvetkovic, Aleksandar, Dalvie, Neil C., Liu, Diana, Liu, Yanjun, Mascarenhas, Craig A., Matthews, Catherine B., Mozdzierz, Nicholas J., Shah, Kartik A., Wu, Shiaw-Lin, Hancock, William S., Braatz, Richard D., Cramer, Steven M., Love, J. Christopher
Publicado 2018

A modular protein subunit vaccine candidate produced in yeast confers protection against SARS-CoV-2 in non-human primates

por Dalvie, Neil C., Tostanoski, Lisa H., Rodriguez-Aponte, Sergio A, Kaur, Kawaljit, Bajoria, Sakshi, Kumru, Ozan S., Martinot, Amanda J., Chandrashekar, Abishek, McMahan, Katherine, Mercado, Noe B., Yu, Jingyou, Chang, Aiquan, Giffin, Victoria M., Nampanya, Felix, Patel, Shivani, Bowman, Lesley, Naranjo, Christopher A., Yun, Dongsoo, Flinchbaugh, Zach, Pessaint, Laurent, Brown, Renita, Velasco, Jason, Teow, Elyse, Cook, Anthony, Andersen, Hanne, Lewis, Mark G., Camp, Danielle L., Silverman, Judith Maxwell, Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Biswas, Sumi, Love, J. Christopher, Barouch, Dan H.
Publicado 2021

SARS-CoV-2 receptor binding domain displayed on HBsAg virus–like particles elicits protective immunity in macaques

por Dalvie, Neil C., Tostanoski, Lisa H., Rodriguez-Aponte, Sergio A., Kaur, Kawaljit, Bajoria, Sakshi, Kumru, Ozan S., Martinot, Amanda J., Chandrashekar, Abishek, McMahan, Katherine, Mercado, Noe B., Yu, Jingyou, Chang, Aiquan, Giffin, Victoria M., Nampanya, Felix, Patel, Shivani, Bowman, Lesley, Naranjo, Christopher A., Yun, Dongsoo, Flinchbaugh, Zach, Pessaint, Laurent, Brown, Renita, Velasco, Jason, Teow, Elyse, Cook, Anthony, Andersen, Hanne, Lewis, Mark G., Camp, Danielle L., Silverman, Judith Maxwell, Nagar, Gaurav S., Rao, Harish D., Lothe, Rakesh R., Chandrasekharan, Rahul, Rajurkar, Meghraj P., Shaligram, Umesh S., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Biswas, Sumi, Love, J. Christopher, Barouch, Dan H.
Publicado 2022

Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters

por Dalvie, Neil C., Rodriguez-Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan, Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Celia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dongsoo, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanne, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith Maxwell, Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice M., Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher
Publicado 2021