Combining optogenetics with sensitive FRET imaging to monitor local microtubule manipulations

por Van Geel, Orry, Cheung, Stephanie, Gadella, Theodorus W. J.
Publicado 2020

Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins

por Van Geel, Orry, Hartsuiker, Roland, Gadella, Theodorus W. J.
Publicado 2018

A mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range

por Klarenbeek, Jeffrey B., Goedhart, Joachim, Hink, Mark A., Gadella, Theodorus W. J., Jalink, Kees
Publicado 2011

Signaling efficiency of Gαq through its effectors p63RhoGEF and GEFT depends on their subcellular location

por Goedhart, Joachim, van Unen, Jakobus, Adjobo-Hermans, Merel J. W., Gadella, Theodorus W. J.
Publicado 2013

Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

por Mastop, Marieke, Bindels, Daphne S., Shaner, Nathan C., Postma, Marten, Gadella, Theodorus W. J., Goedhart, Joachim
Publicado 2017

Kinetics of recruitment and allosteric activation of ARHGEF25 isoforms by the heterotrimeric G-protein Gαq

por van Unen, Jakobus, Yin, Taofei, Wu, Yi I., Mastop, Marieke, Gadella, Theodorus W. J., Goedhart, Joachim
Publicado 2016

Single-cell imaging of ERK and Akt activation dynamics and heterogeneity induced by G-protein-coupled receptors

por Chavez-Abiega, Sergei, Grönloh, Max L. B., Gadella, Theodorus W. J., Bruggeman, Frank J., Goedhart, Joachim
Publicado 2022

Sensitive Detection of p65 Homodimers Using Red-Shifted and Fluorescent Protein-Based FRET Couples

por Goedhart, Joachim, Vermeer, Joop E. M., Adjobo-Hermans, Merel J. W., van Weeren, Laura, Gadella, Theodorus W. J.
Publicado 2007

Quantitative Co-Expression of Proteins at the Single Cell Level – Application to a Multimeric FRET Sensor

por Goedhart, Joachim, van Weeren, Laura, Adjobo-Hermans, Merel J.W., Elzenaar, Ies, Hink, Mark A., Gadella, Theodorus W.J.
Publicado 2011

Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization

por van Unen, Jakobus, Reinhard, Nathalie R., Yin, Taofei, Wu, Yi I., Postma, Marten, Gadella, Theodorus W.J., Goedhart, Joachim
Publicado 2015

The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling

por van Unen, J., Botman, D., Yin, T., Wu, Y. I., Hink, M. A., Gadella, T. W. J., Postma, M., Goedhart, J.
Publicado 2018

The fidelity of stochastic single-molecule super-resolution reconstructions critically depends upon robust background estimation

por Hoogendoorn, Eelco, Crosby, Kevin C., Leyton-Puig, Daniela, Breedijk, Ronald M. P., Jalink, Kees, Gadella, Theodorus W. J., Postma, Marten
Publicado 2014

The balance between Gα(i)-Cdc42/Rac and Gα(1)(2)/(1)(3)-RhoA pathways determines endothelial barrier regulation by sphingosine-1-phosphate

por Reinhard, Nathalie R., Mastop, Marieke, Yin, Taofei, Wu, Yi, Bosma, Esmeralda K., Gadella, Theodorus W. J., Goedhart, Joachim, Hordijk, Peter L.
Publicado 2017

Optimizing FRET-FLIM Labeling Conditions to Detect Nuclear Protein Interactions at Native Expression Levels in Living Arabidopsis Roots

por Long, Yuchen, Stahl, Yvonne, Weidtkamp-Peters, Stefanie, Smet, Wouter, Du, Yujuan, Gadella, Theodorus W. J., Goedhart, Joachim, Scheres, Ben, Blilou, Ikram
Publicado 2018

Quantification of Dark Protein Populations in Fluorescent Proteins by Two-Color Coincidence Detection and Nanophotonic Manipulation

por Heesink, Gobert, Caron, Cécile, van Leijenhorst-Groener, Kirsten, Molenaar, Robert, Gadella, Theodorus W. J., Claessens, Mireille M. A. E., Blum, Christian
Publicado 2022

A New Generation of FRET Sensors for Robust Measurement of Gα(i1), Gα(i2) and Gα(i3) Activation Kinetics in Single Cells

por van Unen, Jakobus, Stumpf, Anette D., Schmid, Benedikt, Reinhard, Nathalie R., Hordijk, Peter L., Hoffmann, Carsten, Gadella, Theodorus W. J., Goedhart, Joachim
Publicado 2016

Agonist-controlled competition of RAR and VDR nuclear receptors for heterodimerization with RXR is manifested in their DNA binding

por Rehó, Bálint, Fadel, Lina, Brazda, Peter, Benziane, Anass, Hegedüs, Éva, Sen, Pialy, Gadella, Theodorus W.J., Tóth, Katalin, Nagy, László, Vámosi, György
Publicado 2023

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

por Goedhart, Joachim, von Stetten, David, Noirclerc-Savoye, Marjolaine, Lelimousin, Mickaël, Joosen, Linda, Hink, Mark A., van Weeren, Laura, Gadella, Theodorus W.J., Royant, Antoine
Publicado 2012

Interaction of Medicago truncatula Lysin Motif Receptor-Like Kinases, NFP and LYK3, Produced in Nicotiana benthamiana Induces Defence-Like Responses

por Pietraszewska-Bogiel, Anna, Lefebvre, Benoit, Koini, Maria A., Klaus-Heisen, Dörte, Takken, Frank L. W., Geurts, René, Cullimore, Julie V., Gadella, Theodorus W.J.
Publicado 2013

Spatiotemporal analysis of RhoA/B/C activation in primary human endothelial cells

por Reinhard, Nathalie R., van Helden, Suzanne F., Anthony, Eloise C., Yin, Taofei, Wu, Yi I., Goedhart, Joachim, Gadella, Theodorus W. J., Hordijk, Peter L.
Publicado 2016