Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?

por Greenbaum, Alon, Jang, Min J., Challis, Collin, Gradinaru, Viviana
Publicado 2017

Targeting the lung epithelium after intravenous delivery by directed evolution of underexplored sites on the AAV capsid

por Goertsen, David, Goeden, Nick, Flytzanis, Nicholas C., Gradinaru, Viviana
Publicado 2022

Immediate responses to ambient light in vivo reveal distinct subpopulations of suprachiasmatic VIP neurons

por Kahan, Anat, Mahe, Karan, Dutta, Sayan, Kassraian, Pegah, Wang, Alexander, Gradinaru, Viviana
Publicado 2023

Structural basis of receptor usage by the engineered capsid AAV-PHP.eB

por Jang, Seongmin, Shen, Hao K., Ding, Xiaozhe, Miles, Timothy F., Gradinaru, Viviana
Publicado 2022

Machine learning to design integral membrane channelrhodopsins for efficient eukaryotic expression and plasma membrane localization

por Bedbrook, Claire N., Yang, Kevin K., Rice, Austin J., Gradinaru, Viviana, Arnold, Frances H.
Publicado 2017

Deep Parallel Characterization of AAV Tropism and AAV-Mediated Transcriptional Changes via Single-Cell RNA Sequencing

por Brown, David, Altermatt, Michael, Dobreva, Tatyana, Chen, Sisi, Wang, Alexander, Thomson, Matt, Gradinaru, Viviana
Publicado 2021

Advances in AAV technology for delivering genetically encoded cargo to the nonhuman primate nervous system

por Campos, Lillian J., Arokiaraj, Cynthia M., Chuapoco, Miguel R., Chen, Xinhong, Goeden, Nick, Gradinaru, Viviana, Fox, Andrew S.
Publicado 2023

Machine learning-guided channelrhodopsin engineering enables minimally-invasive optogenetics

por Bedbrook, Claire N., Yang, Kevin K., Robinson, J. Elliott, Mackey, Elisha D., Gradinaru, Viviana, Arnold, Frances H.
Publicado 2019

Exposing the Three-Dimensional Biogeography and Metabolic States of Pathogens in Cystic Fibrosis Sputum via Hydrogel Embedding, Clearing, and rRNA Labeling

por DePas, William H., Starwalt-Lee, Ruth, Van Sambeek, Lindsey, Ravindra Kumar, Sripriya, Gradinaru, Viviana, Newman, Dianne K.
Publicado 2016

Deep tissue optical focusing and optogenetic modulation with time-reversed ultrasonically encoded light

por Ruan, Haowen, Brake, Joshua, Robinson, J. Elliott, Liu, Yan, Jang, Mooseok, Xiao, Cheng, Zhou, Chunyi, Gradinaru, Viviana, Yang, Changhuei
Publicado 2017

Genomic Reconstruction of an Uncultured Hydrothermal Vent Gammaproteobacterial Methanotroph (Family Methylothermaceae) Indicates Multiple Adaptations to Oxygen Limitation

por Skennerton, Connor T., Ward, Lewis M., Michel, Alice, Metcalfe, Kyle, Valiente, Chanel, Mullin, Sean, Chan, Ken Y., Gradinaru, Viviana, Orphan, Victoria J.
Publicado 2015

CRISPR-clear imaging of melanin-rich B16-derived solid tumors

por Schubert, Rajib, Bae, Taegeun, Simic, Branko, Smith, Sheena N., Park, Seong-Ho, Nagy-Davidescu, Gabriela, Gradinaru, Viviana, Plückthun, Andreas, Hur, Junho K.
Publicado 2023

Spatial transcriptomics for profiling the tropism of viral vectors in tissues

por Jang, Min J., Coughlin, Gerard M., Jackson, Cameron R., Chen, Xinhong, Chuapoco, Miguel R., Vendemiatti, Julia L., Wang, Alexander Z., Gradinaru, Viviana
Publicado 2023

Optogenetic Delay of Status Epilepticus Onset in an In Vivo Rodent Epilepsy Model

por Sukhotinsky, Inna, Chan, Alexander M., Ahmed, Omar J., Rao, Vikram R., Gradinaru, Viviana, Ramakrishnan, Charu, Deisseroth, Karl, Majewska, Ania K., Cash, Sydney S.
Publicado 2013

Use of high-content imaging to quantify transduction of AAV-PHP viruses in the brain following systemic delivery

por Smith, Edward J, Farshim, Pamela P, Flomen, Rachel, Jones, Samuel T, McAteer, Sean J, Deverman, Benjamin E, Gradinaru, Viviana, Bates, Gillian P
Publicado 2021

Light-guided sectioning for precise in situ localization and tissue interface analysis for brain-implanted optical fibers and GRIN lenses

por Kahan, Anat, Greenbaum, Alon, Jang, Min J., Robinson, J. Elliott, Ryan Cho, Jounhong, Chen, Xinhong, Kassraian, Pegah, Wagenaar, Daniel A., Gradinaru, Viviana
Publicado 2021

Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1

por Robinson, J Elliott, Coughlin, Gerard M, Hori, Acacia M, Cho, Jounhong Ryan, Mackey, Elisha D, Turan, Zeynep, Patriarchi, Tommaso, Tian, Lin, Gradinaru, Viviana
Publicado 2019

Archaerhodopsin Variants with Enhanced Voltage Sensitive Fluorescence in Mammalian and Caenorhabditis elegans Neurons

por Flytzanis, Nicholas C., Bedbrook, Claire N., Chiu, Hui, Engqvist, Martin K. M., Xiao, Cheng, Chan, Ken Y., Sternberg, Paul W., Arnold, Frances H., Gradinaru, Viviana
Publicado 2014

Gut-seeded α-synuclein fibrils promote gut dysfunction and brain pathology specifically in aged mice

por Challis, Collin, Hori, Acacia, Sampson, Timothy R., Yoo, Bryan B., Challis, Rosemary C., Hamilton, Adam M., Mazmanian, Sarkis K., Volpicelli-Daley, Laura A., Gradinaru, Viviana
Publicado 2020

Removal of a partial genomic duplication restores synaptic transmission and behavior in the MyosinVA mutant mouse Flailer

por Bustos, Fernando J., Pandian, Swarna, Haensgen, Henny, Zhao, Jian-Ping, Strouf, Haley, Heidenreich, Matthias, Swiech, Lukasz, Deverman, Benjamin E., Gradinaru, Viviana, Zhang, Feng, Constantine-Paton, Martha
Publicado 2023