Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria

por Taylor, George M, Hitchcock, Andrew, Heap, John T
Publicado 2021

Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering

por Taylor, George M, Mordaka, Paweł M, Heap, John T
Publicado 2019

Versatile selective evolutionary pressure using synthetic defect in universal metabolism

por Sellés Vidal, Lara, Murray, James W., Heap, John T.
Publicado 2021

A primer to directed evolution: current methodologies and future directions

por Sellés Vidal, Lara, Isalan, Mark, Heap, John T., Ledesma-Amaro, Rodrigo
Publicado 2023

A genetic assay for gene essentiality in Clostridium

por Walker, David J.F., Heap, John T., Winzer, Klaus, Minton, Nigel P.
Publicado 2016

VAPPER: High-throughput variant antigen profiling in African trypanosomes of livestock

por Silva Pereira, Sara, Heap, John, Jones, Andrew R, Jackson, Andrew P
Publicado 2019

Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways

por Moreira, João P. C., Heap, John T., Alves, Joana I., Domingues, Lucília
Publicado 2023

In Vitro Susceptibility to Closthioamide among Clinical and Reference Strains of Neisseria gonorrhoeae

por Miari, Victoria F., Solanki, Priya, Hleba, Yonek, Stabler, Richard A., Heap, John T.
Publicado 2017

Transcriptional Terminators Allow Leak-Free Chromosomal Integration of Genetic Constructs in Cyanobacteria

por Kelly, Ciarán L., Taylor, George M., Šatkutė, Aistė, Dekker, Linda, Heap, John T.
Publicado 2019

Two-Component Signal Transduction System CBO0787/CBO0786 Represses Transcription from Botulinum Neurotoxin Promoters in Clostridium botulinum ATCC 3502

por Zhang, Zhen, Korkeala, Hannu, Dahlsten, Elias, Sahala, Elina, Heap, John T., Minton, Nigel P., Lindström, Miia
Publicado 2013

Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824

por Ehsaan, Muhammad, Kuit, Wouter, Zhang, Ying, Cartman, Stephen T., Heap, John T., Winzer, Klaus, Minton, Nigel P.
Publicado 2016

Synthetic negative feedback circuits using engineered small RNAs

por Kelly, Ciarán L, Harris, Andreas W K, Steel, Harrison, Hancock, Edward J, Heap, John T, Papachristodoulou, Antonis
Publicado 2018

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

por Heap, John T., Ehsaan, Muhammad, Cooksley, Clare M., Ng, Yen-Kuan, Cartman, Stephen T., Winzer, Klaus, Minton, Nigel P.
Publicado 2012

Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824

por Kovács, Katalin, Willson, Benjamin J, Schwarz, Katrin, Heap, John T, Jackson, Adam, Bolam, David N, Winzer, Klaus, Minton, Nigel P
Publicado 2013

Inactivation of the dnaK gene in Clostridium difficile 630 Δerm yields a temperature-sensitive phenotype and increases biofilm-forming ability

por Jain, Shailesh, Smyth, Deborah, O’Hagan, Barry M. G., Heap, John T., McMullan, Geoff, Minton, Nigel P., Ternan, Nigel G.
Publicado 2017

A combinatorial DNA assembly approach to biosynthesis of N-linked glycans in E. coli

por Passmore, Ian J, Faulds-Pain, Alexandra, Abouelhadid, Sherif, Harrison, Mark A, Hall, Catherine L, Hitchen, Paul, Dell, Anne, Heap, John T, Wren, Brendan W
Publicado 2023

Spores of Clostridium engineered for clinical efficacy and safety cause regression and cure of tumors in vivo

por Heap, John T., Theys, Jan, Ehsaan, Muhammad, Kubiak, Aleksandra M, Dubois, Ludwig, Paesmans, Kim, Van Mellaert, Lieve, Knox, Richard, Kuehne, Sarah A., Lambin, Phillipe, Minton, Nigel P.
Publicado 2014

Type IV Pili-Independent Photocurrent Production by the Cyanobacterium Synechocystis sp. PCC 6803

por Thirumurthy, Miyuki A., Hitchcock, Andrew, Cereda, Angelo, Liu, Jiawei, Chavez, Marko S., Doss, Bryant L., Ros, Robert, El-Naggar, Mohamed Y., Heap, John T., Bibby, Thomas S., Jones, Anne K.
Publicado 2020