An efficient method to assemble linear DNA templates for in vitro screening and selection systems

por Stein, Viktor, Hollfelder, Florian
Publicado 2009

“NAD‐display”: Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry

por Lindenburg, Laurens, Hollfelder, Florian
Publicado 2021

Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput

por Holstein, Josephin M., Gylstorff, Christian, Hollfelder, Florian
Publicado 2021

Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity

por Kaltenbach, Miriam, Emond, Stephane, Hollfelder, Florian, Tokuriki, Nobuhiko
Publicado 2016

Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context‐Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Spac...

por Bayer, Christopher D., van Loo, Bert, Hollfelder, Florian
Publicado 2017

Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays

por Huovinen, Tuomas, Lindenburg, Laurens, Minter, Ralph, Hollfelder, Florian
Publicado 2021

Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies

por Medcalf, Elliot J., Gantz, Maximilian, Kaminski, Tomasz S., Hollfelder, Florian
Publicado 2023

In vitro affinity screening of protein and peptide binders by megavalent bead surface display

por Diamante, Letizia, Gatti-Lafranconi, Pietro, Schaerli, Yolanda, Hollfelder, Florian
Publicado 2013

Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process

por Houlihan, Gillian, Gatti-Lafranconi, Pietro, Lowe, David, Hollfelder, Florian
Publicado 2015

Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease

por Shafee, Thomas, Gatti-Lafranconi, Pietro, Minter, Ralph, Hollfelder, Florian
Publicado 2015

Bioinspired genotype–phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins

por van Vliet, Liisa D., Colin, Pierre-Yves, Hollfelder, Florian
Publicado 2015

High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform

por Neun, Stefanie, van Vliet, Liisa, Hollfelder, Florian, Gielen, Fabrice
Publicado 2022

Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets

por Ladeveze, Simon, Zurek, Paul J., Kaminski, Tomasz S., Emond, Stephane, Hollfelder, Florian
Publicado 2023

A single mutation in the core domain of the lac repressor reduces leakiness

por Gatti-Lafranconi, Pietro, Dijkman, Willem P, Devenish, Sean RA, Hollfelder, Florian
Publicado 2013

UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

por Zurek, Paul Jannis, Knyphausen, Philipp, Neufeld, Katharina, Pushpanath, Ahir, Hollfelder, Florian
Publicado 2020

Exploiting protease activation for therapy

por Bleuez, Chloe, Koch, Wolfgang F., Urbach, Carole, Hollfelder, Florian, Jermutus, Lutz
Publicado 2022

Peptide Dendrimer/Lipid Hybrid Systems Are Efficient DNA Transfection Reagents: Structure–Activity Relationships Highlight the Role of Charge Distribution Across Dendrimer Generati...

por Kwok, Albert, Eggimann, Gabriela A., Reymond, Jean-Louis, Darbre, Tamis, Hollfelder, Florian
Publicado 2013

Reverse evolution leads to genotypic incompatibility despite functional and active site convergence

por Kaltenbach, Miriam, Jackson, Colin J, Campbell, Eleanor C, Hollfelder, Florian, Tokuriki, Nobuhiko
Publicado 2015

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

por Gantz, Maximilian, Neun, Stefanie, Medcalf, Elliot J., van Vliet, Liisa D., Hollfelder, Florian
Publicado 2023

Generation of liver mesenchyme and ductal cell organoid co-culture using cell self-aggregation and droplet microfluidics

por Dowbaj, Anna M., Kohler, Timo N., Cordero-Espinoza, Lucía, Hollfelder, Florian, Huch, Meritxell
Publicado 2023