The monomeric GIY-YIG homing endonuclease I-BmoI uses a molecular anchor and a flexible tether to sequentially nick DNA

por Kleinstiver, Benjamin P., Wolfs, Jason M., Edgell, David R.
Publicado 2013

A unified genetic, computational and experimental framework identifies functionally relevant residues of the homing endonuclease I-BmoI

por Kleinstiver, Benjamin P., Fernandes, Andrew D., Gloor, Gregory B., Edgell, David R.
Publicado 2010

Divalent Metal Ion Differentially Regulates the Sequential Nicking Reactions of the GIY-YIG Homing Endonuclease I-BmoI

por Kleinstiver, Benjamin P., Bérubé-Janzen, Wesley, Fernandes, Andrew D., Edgell, David R.
Publicado 2011

Estimating the evidence of selection and the reliability of inference in unigenic evolution

por Fernandes, Andrew D, Kleinstiver, Benjamin P, Edgell, David R, Wahl, Lindi M, Gloor, Gregory B
Publicado 2010

enAsCas12a Enables CRISPR-Directed Evolution to Screen for Functional Drug Resistance Mutations in Sequences Inaccessible to SpCas9

por Neggers, Jasper Edgar, Jacquemyn, Maarten, Dierckx, Tim, Kleinstiver, Benjamin Peter, Thibaut, Hendrik Jan, Daelemans, Dirk
Publicado 2021

Multimodal CRISPR perturbations of GWAS loci associated with coronary artery disease in vascular endothelial cells

por Wünnemann, Florian, Fotsing Tadjo, Thierry, Beaudoin, Mélissa, Lalonde, Simon, Lo, Ken Sin, Kleinstiver, Benjamin P., Lettre, Guillaume
Publicado 2023

High-fidelity CRISPR-Cas9 variants with undetectable genome-wide off-targets

por Kleinstiver, Benjamin P., Pattanayak, Vikram, Prew, Michelle S., Tsai, Shengdar Q., Nguyen, Nhu, Zheng, Zongli, Joung, J. Keith
Publicado 2016

Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer

por Mahendra, Caroline, Christie, Kathleen A., Osuna, Beatriz A., Pinilla-Redondo, Rafael, Kleinstiver, Benjamin P., Bondy-Denomy, Joseph
Publicado 2020

Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases

por da Silva, Joana Ferreira, Tou, Connor J., King, Emily M., Eller, Madeline L., Ma, Linyuan, Rufino-Ramos, David, Kleinstiver, Benjamin P.
Publicado 2023

Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors

por Tak, Y. Esther, Kleinstiver, Benjamin P., Nuñez, James K., Hsu, Jonathan Y., Horng, Joy E., Gong, Jingyi, Weissman, Jonathan S., Joung, J. Keith
Publicado 2017

In vivo engineering of lymphocytes after systemic exosome-associated AAV delivery

por Breuer, Cort B., Hanlon, Killian S., Natasan, Jeya-shree, Volak, Adrienn, Meliani, Amine, Mingozzi, Federico, Kleinstiver, Benjamin P., Moon, James J., Maguire, Casey A.
Publicado 2020

The I-TevI Nuclease and Linker Domains Contribute to the Specificity of Monomeric TALENs

por Kleinstiver, Benjamin P., Wang, Li, Wolfs, Jason M., Kolaczyk, Tomasz, McDowell, Brendon, Wang, Xu, Schild-Poulter, Caroline, Bogdanove, Adam J., Edgell, David R.
Publicado 2014

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

por Kleinstiver, Benjamin P., Tsai, Shengdar Q., Prew, Michelle S., Nguyen, Nhu T., Welch, Moira M., Lopez, Jose M., McCaw, Zachary R., Aryee, Martin J., Joung, J. Keith
Publicado 2016

Temporal and Spatial Post-Transcriptional Regulation of Zebrafish tie1 mRNA by Long Noncoding RNA During Brain Vascular Assembly

por Chowdhury, Tamjid A., Koceja, Chris, Eisa-Beygi, Shahram, Kleinstiver, Benjamin P., Kumar, Suresh N., Lin, Chien-Wei, Li, Keguo, Prabhudesai, Shubhangi, Joung, J. Keith, Ramchandran, Ramani
Publicado 2018

Allele-specific silencing of the gain-of-function mutation in Huntington’s disease using CRISPR/Cas9

por Shin, Jun Wan, Hong, Eun Pyo, Park, Seri S., Choi, Doo Eun, Seong, Ihn Sik, Whittaker, Madelynn N., Kleinstiver, Benjamin P., Chen, Richard Z., Lee, Jong-Min
Publicado 2022

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy

por Chen, Janice S., Dagdas, Yavuz S., Kleinstiver, Benjamin P., Welch, Moira M., Sousa, Alexander A., Harrington, Lucas B., Sternberg, Samuel H., Joung, J. Keith, Yildiz, Ahmet, Doudna, Jennifer A.
Publicado 2017

Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize

por Lee, Keunsub, Zhang, Yingxiao, Kleinstiver, Benjamin P., Guo, Jimmy A., Aryee, Martin J., Miller, Jonah, Malzahn, Aimee, Zarecor, Scott, Lawrence‐Dill, Carolyn J., Joung, J. Keith, Qi, Yiping, Wang, Kan
Publicado 2018

CRISPR-Cas knockout of miR21 reduces glioma growth

por Nieland, Lisa, van Solinge, Thomas S., Cheah, Pike See, Morsett, Liza M., El Khoury, Joseph, Rissman, Joseph I., Kleinstiver, Benjamin P., Broekman, Marike L.D., Breakefield, Xandra O., Abels, Erik R.
Publicado 2022

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

por Kleinstiver, Benjamin P., Prew, Michelle S., Tsai, Shengdar Q., Topkar, Ved, Nguyen, Nhu T., Zheng, Zongli, Gonzales, Andrew P.W., Li, Zhuyun, Peterson, Randall T., Yeh, Jing-Ruey Joanna, Aryee, Martin J., Joung, J. Keith
Publicado 2015

Camptothecin resistance is determined by the regulation of topoisomerase I degradation mediated by ubiquitin proteasome pathway

por Ando, Koji, Shah, Ankur K., Sachdev, Vibhu, Kleinstiver, Benjamin P., Taylor-Parker, Julian, Welch, Moira M., Hu, Yiheng, Salgia, Ravi, White, Forest M., Parvin, Jeffrey D., Ozonoff, Al, Rameh, Lucia E., Joung, J. Keith, Bharti, Ajit K.
Publicado 2017