Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

por Dalvie, Neil C., Naranjo, Christopher A., Rodriguez-Aponte, Sergio A., Johnston, Ryan S., Christopher Love, J.
Publicado 2022

Modular development enables rapid design of media for alternative hosts

por Biedermann, Andrew M., Gengaro, Isabella R., Rodriguez‐Aponte, Sergio A., Love, Kerry R., Love, J. Christopher
Publicado 2021

Nanoalum Formulations Containing Aluminum Hydroxide and CpG 1018(TM) Adjuvants: The Effect on Stability and Immunogenicity of a Recombinant SARS-CoV-2 RBD Antigen

por Bajoria, Sakshi, Kumru, Ozan S., Doering, Jennifer, Berman, Katherine, Slyke, Greta Van, Prigodich, Anneka, Rodriguez-Aponte, Sergio A., Kleanthous, Harry, Love, J. Christopher, Mantis, Nicholas J., Joshi, Sangeeta B., Volkin, David B.
Publicado 2023

Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein Expressed in Yeast

por Nagar, Gaurav, Jain, Siddharth, Rajurkar, Meghraj, Lothe, Rakesh, Rao, Harish, Majumdar, Sourav, Gautam, Manish, Rodriguez-Aponte, Sergio A., Crowell, Laura E., Love, J. Christopher, Dandekar, Prajakta, Puranik, Amita, Gairola, Sunil, Shaligram, Umesh, Jain, Ratnesh
Publicado 2023

Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii

por Dalvie, Neil C., Biedermann, Andrew M., Rodriguez-Aponte, Sergio A., Naranjo, Christopher A., Rao, Harish D., Rajurkar, Meghraj P., Lothe, Rakesh R., Shaligram, Umesh S., Johnston, Ryan S., Crowell, Laura E., Castelino, Seraphin, Tracey, Mary Kate, Whittaker, Charles A., Love, J. Christopher
Publicado 2021

Scalable, methanol‐free manufacturing of the SARS‐CoV‐2 receptor‐binding domain in engineered Komagataella phaffii

por Dalvie, Neil C., Biedermann, Andrew M., Rodriguez‐Aponte, Sergio A., Naranjo, Christopher A., Rao, Harish D., Rajurkar, Meghraj P., Lothe, Rakesh R., Shaligram, Umesh S., Johnston, Ryan S., Crowell, Laura E., Castelino, Seraphin, Tracey, Mary K., Whittaker, Charles A., Love, J. Christopher
Publicado 2021

Antigen-adjuvant interactions, stability, and immunogenicity profiles of a SARS-CoV-2 receptor-binding domain (RBD) antigen formulated with aluminum salt and CpG adjuvants

por Bajoria, Sakshi, Kaur, Kawaljit, Kumru, Ozan S., Van Slyke, Greta, Doering, Jennifer, Novak, Hayley, Rodriguez Aponte, Sergio A., Dalvie, Neil C., Naranjo, Christopher A., Johnston, Ryan S., Silverman, Judith Maxwell, Kleanthous, Harry, Love, J. Christopher, Mantis, Nicholas J., Joshi, Sangeeta B., Volkin, David B.
Publicado 2022

Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

por Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra D., Rodriguez-Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina, Mukhopadhyay, Tarit K., Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
Publicado 2021

Phosphate-mediated coanchoring of RBD immunogens and molecular adjuvants to alum potentiates humoral immunity against SARS-CoV-2

por Rodrigues, Kristen A., Rodriguez-Aponte, Sergio A., Dalvie, Neil C., Lee, Jeong Hyun, Abraham, Wuhbet, Carnathan, Diane G., Jimenez, Luis E., Ngo, Julia T., Chang, Jason Y. H., Zhang, Zeli, Yu, Jingyou, Chang, Aiquan, Nakao, Catherine, Goodwin, Benjamin, Naranjo, Christopher A., Zhang, Libin, Silva, Murillo, Barouch, Dan H., Silvestri, Guido, Crotty, Shane, Love, J. Christopher, Irvine, Darrell J.
Publicado 2021

RBD-VLP Vaccines Adjuvanted with Alum or SWE Protect K18-hACE2 Mice against SARS-CoV-2 VOC Challenge

por Wong, Ting Y., Russ, Brynnan P., Lee, Katherine S., Miller, Olivia A., Kang, Jason, Cooper, Melissa, Winters, Michael T., Rodriguez-Aponte, Sergio A., Dalvie, Neil C., Johnston, Ryan S., Rader, Nathaniel A., Wong, Zeriel Y., Cyphert, Holly A., Martinez, Ivan, Shaligram, Umesh, Batwal, Saurabh, Lothe, Rakesh, Chandrasekaran, Rahul, Nagar, Gaurav, Rajurkar, Meghraj, Rao, Harish, Bevere, Justin R., Barbier, Mariette, Love, J. Christopher, Damron, F. Heath
Publicado 2022

Intranasal vaccination with lipid-conjugated immunogens promotes antigen transmucosal uptake to drive mucosal and systemic immunity

por Hartwell, Brittany L., Melo, Mariane B., Xiao, Peng, Lemnios, Ashley A., Li, Na, Chang, Jason Y.H., Yu, Jingyou, Gebre, Makda S., Chang, Aiquan, Maiorino, Laura, Carter, Crystal, Moyer, Tyson J., Dalvie, Neil C., Rodriguez-Aponte, Sergio A., Rodrigues, Kristen A., Silva, Murillo, Suh, Heikyung, Adams, Josetta, Fontenot, Jane, Love, J. Christopher, Barouch, Dan H., Villinger, Francois, Ruprecht, Ruth M., Irvine, Darrell J.
Publicado 2022

Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variants

por Rodriguez-Aponte, Sergio A., Dalvie, Neil C., Wong, Ting Y., Johnston, Ryan S., Naranjo, Christopher A., Bajoria, Sakshi, Kumru, Ozan S., Kaur, Kawaljit, Russ, Brynnan P., Lee, Katherine S., Cyphert, Holly A., Barbier, Mariette, Rao, Harish D., Rajurkar, Meghraj P., Lothe, Rakesh R., Shaligram, Umesh S., Batwal, Saurabh, Chandrasekaran, Rahul, Nagar, Gaurav, Kleanthous, Harry, Biswas, Sumi, Bevere, Justin R., Joshi, Sangeeta B., Volkin, David B., Damron, F. Heath, Love, J. Christopher
Publicado 2023

A modular protein subunit vaccine candidate produced in yeast confers protection against SARS-CoV-2 in non-human primates

por Dalvie, Neil C., Tostanoski, Lisa H., Rodriguez-Aponte, Sergio A, Kaur, Kawaljit, Bajoria, Sakshi, Kumru, Ozan S., Martinot, Amanda J., Chandrashekar, Abishek, McMahan, Katherine, Mercado, Noe B., Yu, Jingyou, Chang, Aiquan, Giffin, Victoria M., Nampanya, Felix, Patel, Shivani, Bowman, Lesley, Naranjo, Christopher A., Yun, Dongsoo, Flinchbaugh, Zach, Pessaint, Laurent, Brown, Renita, Velasco, Jason, Teow, Elyse, Cook, Anthony, Andersen, Hanne, Lewis, Mark G., Camp, Danielle L., Silverman, Judith Maxwell, Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Biswas, Sumi, Love, J. Christopher, Barouch, Dan H.
Publicado 2021

SARS-CoV-2 receptor binding domain displayed on HBsAg virus–like particles elicits protective immunity in macaques

por Dalvie, Neil C., Tostanoski, Lisa H., Rodriguez-Aponte, Sergio A., Kaur, Kawaljit, Bajoria, Sakshi, Kumru, Ozan S., Martinot, Amanda J., Chandrashekar, Abishek, McMahan, Katherine, Mercado, Noe B., Yu, Jingyou, Chang, Aiquan, Giffin, Victoria M., Nampanya, Felix, Patel, Shivani, Bowman, Lesley, Naranjo, Christopher A., Yun, Dongsoo, Flinchbaugh, Zach, Pessaint, Laurent, Brown, Renita, Velasco, Jason, Teow, Elyse, Cook, Anthony, Andersen, Hanne, Lewis, Mark G., Camp, Danielle L., Silverman, Judith Maxwell, Nagar, Gaurav S., Rao, Harish D., Lothe, Rakesh R., Chandrasekharan, Rahul, Rajurkar, Meghraj P., Shaligram, Umesh S., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Biswas, Sumi, Love, J. Christopher, Barouch, Dan H.
Publicado 2022

Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters

por Dalvie, Neil C., Rodriguez-Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan, Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Celia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dongsoo, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanne, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith Maxwell, Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice M., Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher
Publicado 2021

Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice

por Dalvie, Neil C., Rodriguez-Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Celia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dongsoo, Brunette, Natalie, Wang, Jing Yang, Walkey, Carl, Fiala, Brooke, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanne, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith Maxwell, Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice M., Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher
Publicado 2021