Pervasive generation of oppositely oriented spacers during CRISPR adaptation

por Shmakov, Sergey, Savitskaya, Ekaterina, Semenova, Ekaterina, Logacheva, Maria D., Datsenko, Kirill A., Severinov, Konstantin
Publicado 2014

CRISPR–Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5′ ssDNA exonuclease...

por Radovčić, Marin, Killelea, Tom, Savitskaya, Ekaterina, Wettstein, Lukas, Bolt, Edward L, Ivančić-Baće, Ivana
Publicado 2018

Mapping CRISPR spaceromes reveals vast host-specific viromes of prokaryotes

por Shmakov, Sergey A., Wolf, Yuri I., Savitskaya, Ekaterina, Severinov, Konstantin V., Koonin, Eugene V.
Publicado 2020

The action of Escherichia coli CRISPR–Cas system on lytic bacteriophages with different lifestyles and development strategies

por Strotskaya, Alexandra, Savitskaya, Ekaterina, Metlitskaya, Anastasia, Morozova, Natalia, Datsenko, Kirill A., Semenova, Ekaterina, Severinov, Konstantin
Publicado 2017

Genome Maintenance Proteins Modulate Autoimmunity Mediated Primed Adaptation by the Escherichia coli Type I-E CRISPR-Cas System

por Kurilovich, Elena, Shiriaeva, Anna, Metlitskaya, Anastasia, Morozova, Natalia, Ivancic-Bace, Ivana, Severinov, Konstantin, Savitskaya, Ekaterina
Publicado 2019

Avoidance of Trinucleotide Corresponding to Consensus Protospacer Adjacent Motif Controls the Efficiency of Prespacer Selection during Primed Adaptation

por Musharova, Olga, Vyhovskyi, Danylo, Medvedeva, Sofia, Guzina, Jelena, Zhitnyuk, Yulia, Djordjevic, Marko, Severinov, Konstantin, Savitskaya, Ekaterina
Publicado 2018

Red flag on the white reporter: a versatile insulator abuts the white gene in Drosophila and is omnipresent in mini-white constructs

por Chetverina, Darya, Savitskaya, Ekaterina, Maksimenko, Oksana, Melnikova, Larisa, Zaytseva, Olga, Parshikov, Alexander, Galkin, Alexander V., Georgiev, Pavel
Publicado 2008

Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation

por Musharova, Olga, Klimuk, Evgeny, Datsenko, Kirill A., Metlitskaya, Anastasia, Logacheva, Maria, Semenova, Ekaterina, Severinov, Konstantin, Savitskaya, Ekaterina
Publicado 2017

Uncertainty-aware and interpretable evaluation of Cas9–gRNA and Cas12a–gRNA specificity for fully matched and partially mismatched targets with Deep Kernel Learning

por Kirillov, Bogdan, Savitskaya, Ekaterina, Panov, Maxim, Ogurtsov, Aleksey Y, Shabalina, Svetlana A, Koonin, Eugene V, Severinov, Konstantin V
Publicado 2021

Prespacers formed during primed adaptation associate with the Cas1–Cas2 adaptation complex and the Cas3 interference nuclease–helicase

por Musharova, Olga, Medvedeva, Sofia, Klimuk, Evgeny, Guzman, Noemi Marco, Titova, Daria, Zgoda, Victor, Shiriaeva, Anna, Semenova, Ekaterina, Severinov, Konstantin, Savitskaya, Ekaterina
Publicado 2021

Systematic analysis of Type I‐E Escherichia coli CRISPR‐Cas PAM sequences ability to promote interference and primed adaptation

por Musharova, Olga, Sitnik, Vasily, Vlot, Marnix, Savitskaya, Ekaterina, Datsenko, Kirill A., Krivoy, Andrey, Fedorov, Ivan, Semenova, Ekaterina, Brouns, Stan J. J., Severinov, Konstantin
Publicado 2019

The Cas6e ribonuclease is not required for interference and adaptation by the E. coli type I-E CRISPR-Cas system

por Semenova, Ekaterina, Kuznedelov, Konstantin, Datsenko, Kirill A., Boudry, Pierre M., Savitskaya, Ekaterina E., Medvedeva, Sofia, Beloglazova, Natalia, Logacheva, Maria, Yakunin, Alexander F., Severinov, Konstantin
Publicado 2015

The Digital Ageing Atlas: integrating the diversity of age-related changes into a unified resource

por Craig, Thomas, Smelick, Chris, Tacutu, Robi, Wuttke, Daniel, Wood, Shona H., Stanley, Henry, Janssens, Georges, Savitskaya, Ekaterina, Moskalev, Alexey, Arking, Robert, de Magalhães, João Pedro
Publicado 2014

Foreign DNA acquisition by the I-F CRISPR–Cas system requires all components of the interference machinery

por Vorontsova, Daria, Datsenko, Kirill A., Medvedeva, Sofia, Bondy-Denomy, Joseph, Savitskaya, Ekaterina E., Pougach, Ksenia, Logacheva, Maria, Wiedenheft, Blake, Davidson, Alan R., Severinov, Konstantin, Semenova, Ekaterina
Publicado 2015

Detection of spacer precursors formed in vivo during primed CRISPR adaptation

por Shiriaeva, Anna A., Savitskaya, Ekaterina, Datsenko, Kirill A., Vvedenskaya, Irina O., Fedorova, Iana, Morozova, Natalia, Metlitskaya, Anastasia, Sabantsev, Anton, Nickels, Bryce E., Severinov, Konstantin, Semenova, Ekaterina
Publicado 2019

Altered stoichiometry Escherichia coli Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation

por Kuznedelov, Konstantin, Mekler, Vladimir, Lemak, Sofia, Tokmina-Lukaszewska, Monika, Datsenko, Kirill A., Jain, Ishita, Savitskaya, Ekaterina, Mallon, John, Shmakov, Sergey, Bothner, Brian, Bailey, Scott, Yakunin, Alexander F., Severinov, Konstantin, Semenova, Ekaterina
Publicado 2016