SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e

por Kulcsár, Péter István, Tálas, András, Ligeti, Zoltán, Krausz, Sarah Laura, Welker, Ervin
Publicado 2022

Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs

por Kulcsár, Péter István, Tálas, András, Tóth, Eszter, Nyeste, Antal, Ligeti, Zoltán, Welker, Zsombor, Welker, Ervin
Publicado 2020

BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors

por Tálas, András, Simon, Dorottya A., Kulcsár, Péter I., Varga, Éva, Krausz, Sarah L., Welker, Ervin
Publicado 2021

Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells

por Tóth, Eszter, Weinhardt, Nóra, Bencsura, Petra, Huszár, Krisztina, Kulcsár, Péter I., Tálas, András, Fodor, Elfrieda, Welker, Ervin
Publicado 2016

Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage

por Kulcsár, Péter István, Tálas, András, Huszár, Krisztina, Ligeti, Zoltán, Tóth, Eszter, Weinhardt, Nóra, Fodor, Elfrieda, Welker, Ervin
Publicado 2017

PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells

por Simon, Dorottya Anna, Tálas, András, Kulcsár, Péter István, Biczók, Zsuzsanna, Krausz, Sarah Laura, Várady, György, Welker, Ervin
Publicado 2022

Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs

por Huszár, Krisztina, Welker, Zsombor, Györgypál, Zoltán, Tóth, Eszter, Ligeti, Zoltán, Kulcsár, Péter István, Dancsó, János, Tálas, András, Krausz, Sarah Laura, Varga, Éva, Welker, Ervin
Publicado 2023

A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets

por Kulcsár, Péter István, Tálas, András, Ligeti, Zoltán, Tóth, Eszter, Rakvács, Zsófia, Bartos, Zsuzsa, Krausz, Sarah Laura, Welker, Ágnes, Végi, Vanessza Laura, Huszár, Krisztina, Welker, Ervin
Publicado 2023

Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases

por Tóth, Eszter, Varga, Éva, Kulcsár, Péter István, Kocsis-Jutka, Virág, Krausz, Sarah Laura, Nyeste, Antal, Welker, Zsombor, Huszár, Krisztina, Ligeti, Zoltán, Tálas, András, Welker, Ervin
Publicado 2020

Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants

por Tóth, Eszter, Czene, Bernadett C, Kulcsár, Péter I, Krausz, Sarah L, Tálas, András, Nyeste, Antal, Varga, Éva, Huszár, Krisztina, Weinhardt, Nóra, Ligeti, Zoltán, Borsy, Adrienn É, Fodor, Elfrieda, Welker, Ervin
Publicado 2018

A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs

por Tálas, András, Huszár, Krisztina, Kulcsár, Péter István, Varga, Julia K, Varga, Éva, Tóth, Eszter, Welker, Zsombor, Erdős, Gergely, Pach, Péter Ferenc, Welker, Ágnes, Györgypál, Zoltán, Tusnády, Gábor E, Welker, Ervin
Publicado 2021

A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a ‘self-cleaving’ GFP-expression plasmid

por Tálas, András, Kulcsár, Péter István, Weinhardt, Nóra, Borsy, Adrienn, Tóth, Eszter, Szebényi, Kornélia, Krausz, Sarah Laura, Huszár, Krisztina, Vida, István, Sturm, Ádám, Gordos, Bianka, Hoffmann, Orsolya Ivett, Bencsura, Petra, Nyeste, Antal, Ligeti, Zoltán, Fodor, Elfrieda, Welker, Ervin
Publicado 2017