Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events

por Lin, Qingyi, Le, Quynh Anh, Takebayashi, Koki, Thongkittidilok, Chommanart, Wittayarat, Manita, Hirata, Maki, Tanihara, Fuminori, Otoi, Takeshige
Publicado 2021

Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages

por Le, Quynh Anh, Tanihara, Fuminori, Wittayarat, Manita, Namula, Zhao, Sato, Yoko, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Otoi, Takeshige
Publicado 2021

Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos

por Hirata, Maki, Wittayarat, Manita, Namula, Zhao, Anh Le, Quynh, Lin, Qingyi, Takebayashi, Koki, Thongkittidilok, Chommanart, Tanihara, Fuminori, Otoi, Takeshige
Publicado 2021

Zona pellucida treatment before CRISPR/Cas9‐mediated genome editing of porcine zygotes

por Namula, Zhao, Hirata, Maki, Le, Quynh Anh, Lin, Qingyi, Takebayashi, Koki, Yoshimura, Naoaki, Tanihara, Fuminori, Thongkittidilok, Chommanart, Otoi, Takeshige
Publicado 2021

Improvement of the in vitro fertilization and embryo development using frozen–thawed spermatozoa of microminipigs

por Namula, Zhao, Isumi, Yasuhiro, Sato, Yoko, Le, Quynh Anh, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Tanihara, Fuminori, Thongkittidilok, Chommanart, Otoi, Takeshige
Publicado 2021

Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system

por Namula, Zhao, Wittayarat, Manita, Do, Lanh Thi Kim, Van Nguyen, Thanh, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Tanihara, Fuminori, Otoi, Takeshige
Publicado 2022

Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection

por Namula, Zhao, Le, Quynh Anh, Wittayarat, Manita, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Do, Lanh Thi Kim, Tanihara, Fuminori, Otoi, Takeshige
Publicado 2022

Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods

por Le, Quynh Anh, Wittayarat, Manita, Namula, Zhao, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Tanihara, Fuminori, Do, Lanh Thi Kim, Otoi, Takeshige
Publicado 2022

Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN

por Tanihara, Fuminori, Hirata, Maki, Namula, Zhao, Do, Lanh Thi Kim, Yoshimura, Naoaki, Lin, Qingyi, Takebayashi, Koki, Sakuma, Tetsushi, Yamamoto, Takashi, Otoi, Takeshige
Publicado 2023

Generation of mutant pigs by lipofection-mediated genome editing in embryos

por Hirata, Maki, Wittayarat, Manita, Namula, Zhao, Le, Quynh Anh, Lin, Qingyi, Takebayashi, Koki, Thongkittidilok, Chommanart, Mito, Taro, Tomonari, Sayuri, Tanihara, Fuminori, Otoi, Takeshige
Publicado 2021