Evaluation and Reduction of CRISPR Off-Target Cleavage Events

por Vakulskas, Christopher A., Behlke, Mark A.
Publicado 2019

Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate

por WAKE, Yui, VAKULSKAS, Christopher A., GLENN, Steve E., KANEKO, Takehito
Publicado 2022

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

por Banakar, Raviraj, Schubert, Mollie, Collingwood, Michael, Vakulskas, Christopher, Eggenberger, Alan L., Wang, Kan
Publicado 2020

Global role of the bacterial post-transcriptional regulator CsrA revealed by integrated transcriptomics

por Potts, Anastasia H., Vakulskas, Christopher A., Pannuri, Archana, Yakhnin, Helen, Babitzke, Paul, Romeo, Tony
Publicado 2017

Antagonistic control of the turnover pathway for the global regulatory sRNA CsrB by the CsrA and CsrD proteins

por Vakulskas, Christopher A., Leng, Yuanyuan, Abe, Hazuki, Amaki, Takumi, Okayama, Akihiro, Babitzke, Paul, Suzuki, Kazushi, Romeo, Tony
Publicado 2016

Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes

por Zhang, Liyang, Rube, H Tomas, Vakulskas, Christopher A, Behlke, Mark A, Bussemaker, Harmen J, Pufall, Miles A
Publicado 2020

Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases

por Zhang, Yingxiao, Cheng, Yanhao, Fang, Hong, Roberts, Nathaniel, Zhang, Liyang, Vakulskas, Christopher A., Niedz, Randall P., Culver, James N., Qi, Yiping
Publicado 2022

Efficiency, Specificity and Temperature Sensitivity of Cas9 and Cas12a RNPs for DNA-free Genome Editing in Plants

por Banakar, Raviraj, Schubert, Mollie, Kurgan, Gavin, Rai, Krishan Mohan, Beaudoin, Sarah F., Collingwood, Michael A., Vakulskas, Christopher A., Wang, Kan, Zhang, Feng
Publicado 2022

Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation

por Su, Hang, Wang, Yuanchun, Xu, Jin, Omar, Ahmad A., Grosser, Jude W., Calovic, Milica, Zhang, Liyang, Feng, Yu, Vakulskas, Christopher A., Wang, Nian
Publicado 2023

Integrative FourD omics approach profiles the target network of the carbon storage regulatory system

por Sowa, Steven W., Gelderman, Grant, Leistra, Abigail N., Buvanendiran, Aishwarya, Lipp, Sarah, Pitaktong, Areen, Vakulskas, Christopher A., Romeo, Tony, Baldea, Michael, Contreras, Lydia M.
Publicado 2017

Genomic Targets and Features of BarA-UvrY (-SirA) Signal Transduction Systems

por Zere, Tesfalem R., Vakulskas, Christopher A., Leng, Yuanyuan, Pannuri, Archana, Potts, Anastasia H., Dias, Raquel, Tang, Dongjie, Kolaczkowski, Bryan, Georgellis, Dimitris, Ahmer, Brian M. M., Romeo, Tony
Publicado 2015

Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants

por Zhang, Liyang, Li, Gen, Zhang, Yingxiao, Cheng, Yanhao, Roberts, Nathaniel, Glenn, Steve E., DeZwaan-McCabe, Diane, Rube, H. Tomas, Manthey, Jeff, Coleman, Gary, Vakulskas, Christopher A., Qi, Yiping
Publicado 2023

Efficient Targeted Mutagenesis Mediated by CRISPR-Cas12a Ribonucleoprotein Complexes in Maize

por Dong, Shujie, Qin, Yinping Lucy, Vakulskas, Christopher A., Collingwood, Michael A., Marand, Mariam, Rigoulot, Stephen, Zhu, Ling, Jiang, Yaping, Gu, Weining, Fan, Chunyang, Mangum, Anna, Chen, Zhongying, Yarnall, Michele, Zhong, Heng, Elumalai, Sivamani, Shi, Liang, Que, Qiudeng
Publicado 2021

Propensity of Patient-Derived iPSCs for Retinal Differentiation: Implications for Autologous Cell Replacement

por Cooke, Jessica A, Voigt, Andrew P, Collingwood, Michael A, Stone, Nicholas E, Whitmore, S Scott, DeLuca, Adam P, Burnight, Erin R, Anfinson, Kristin R, Vakulskas, Christopher A, Reutzel, Austin J, Daggett, Heather T, Andorf, Jeaneen L, Stone, Edwin M, Mullins, Robert F, Tucker, Budd A
Publicado 2023

A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human haematopoietic stem and progenitor cells

por Vakulskas, Christopher A., Dever, Daniel P., Rettig, Garrett R., Turk, Rolf, Jacobi, Ashley M., Collingwood, Michael A., Bode, Nicole M., McNeill, Matthew S., Yan, Shuqi, Camarena, Joab, Lee, Ciaran M., Park, So Hyun, Wiebking, Volker, Bak, Rasmus O., Gomez-Ospina, Natalia, Pavel-Dinu, Mara, Sun, Wenchao, Bao, Gang, Porteus, Matthew H., Behlke, Mark A.
Publicado 2018

AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

por Zhang, Liyang, Zuris, John A., Viswanathan, Ramya, Edelstein, Jasmine N., Turk, Rolf, Thommandru, Bernice, Rube, H. Tomas, Glenn, Steve E., Collingwood, Michael A., Bode, Nicole M., Beaudoin, Sarah F., Lele, Swarali, Scott, Sean N., Wasko, Kevin M., Sexton, Steven, Borges, Christopher M., Schubert, Mollie S., Kurgan, Gavin L., McNeill, Matthew S., Fernandez, Cecilia A., Myer, Vic E., Morgan, Richard A., Behlke, Mark A., Vakulskas, Christopher A.
Publicado 2021

Author Correction: AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

por Zhang, Liyang, Zuris, John A., Viswanathan, Ramya, Edelstein, Jasmine N., Turk, Rolf, Thommandru, Bernice, Rube, H. Tomas, Glenn, Steve E., Collingwood, Michael A., Bode, Nicole M., Beaudoin, Sarah F., Lele, Swarali, Scott, Sean N., Wasko, Kevin M., Sexton, Steven, Borges, Christopher M., Schubert, Mollie S., Kurgan, Gavin L., McNeill, Matthew S., Fernandez, Cecilia A., Myer, Vic E., Morgan, Richard A., Behlke, Mark A., Vakulskas, Christopher A.
Publicado 2021