Accelerating the Production of Druggable Targets: Eukaryotic Cell-Free Systems Come into Focus

por Thoring, Lena, Zemella, Anne, Wüstenhagen, Doreen, Kubick, Stefan
Publicado 2019

Cell‐free production of pore forming toxins: Functional analysis of thermostable direct hemolysin from Vibrio parahaemolyticus

por Dondapati, Srujan Kumar, Wüstenhagen, Doreen A, Strauch, Eckhard, Kubick, Stefan
Publicado 2017

High-yield cell-free synthesis of human EGFR by IRES-mediated protein translation in a continuous exchange cell-free reaction format

por Quast, Robert B., Sonnabend, Andrei, Stech, Marlitt, Wüstenhagen, Doreen A., Kubick, Stefan
Publicado 2016

High-yield production of “difficult-to-express” proteins in a continuous exchange cell-free system based on CHO cell lysates

por Thoring, Lena, Dondapati, Srujan K., Stech, Marlitt, Wüstenhagen, Doreen A., Kubick, Stefan
Publicado 2017

Cell-free synthesis of human toll-like receptor 9 (TLR9): Optimization of synthesis conditions and functional analysis

por Dondapati, Srujan Kumar, Pietruschka, Georg, Thoring, Lena, Wüstenhagen, Doreen A., Kubick, Stefan
Publicado 2019

Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of “Difficult-to-Express” Proteins and Future Perspectives

por Thoring, Lena, Wüstenhagen, Doreen A., Borowiak, Maria, Stech, Marlitt, Sonnabend, Andrei, Kubick, Stefan
Publicado 2016

A Continuous-Exchange Cell-Free Protein Synthesis System Based on Extracts from Cultured Insect Cells

por Stech, Marlitt, Quast, Robert B., Sachse, Rita, Schulze, Corina, Wüstenhagen, Doreen A., Kubick, Stefan
Publicado 2014

Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems

por Dondapati, Srujan Kumar, Lübberding, Henning, Zemella, Anne, Thoring, Lena, Wüstenhagen, Doreen A., Kubick, Stefan
Publicado 2019

IRES-Mediated Translation of Membrane Proteins and Glycoproteins in Eukaryotic Cell-Free Systems

por Brödel, Andreas K., Sonnabend, Andrei, Roberts, Lisa O., Stech, Marlitt, Wüstenhagen, Doreen A., Kubick, Stefan
Publicado 2013

Characterization of trh2 Harbouring Vibrio parahaemolyticus Strains Isolated in Germany

por Bechlars, Silke, Jäckel, Claudia, Diescher, Susanne, Wüstenhagen, Doreen A., Kubick, Stefan, Dieckmann, Ralf, Strauch, Eckhard
Publicado 2015

Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin

por Zemella, Anne, Thoring, Lena, Hoffmeister, Christian, Šamalíková, Mária, Ehren, Patricia, Wüstenhagen, Doreen A., Kubick, Stefan
Publicado 2018

Cell-free synthesis of the hirudin variant 1 of the blood-sucking leech Hirudo medicinalis

por Wüstenhagen, Doreen A., Lukas, Phil, Müller, Christian, Aubele, Simone A., Hildebrandt, Jan-Peter, Kubick, Stefan
Publicado 2020

Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems

por Dhandapani, Priyavathi, Dondapati, Srujan Kumar, Zemella, Anne, Bräuer, Dennis, Wüstenhagen, Doreen Anja, Mergler, Stefan, Kubick, Stefan
Publicado 2021

Mammalian cell-free protein expression promotes the functional characterization of the tripartite non-hemolytic enterotoxin from Bacillus cereus

por Ramm, Franziska, Dondapati, Srujan Kumar, Thoring, Lena, Zemella, Anne, Wüstenhagen, Doreen Anja, Frentzel, Hendrik, Stech, Marlitt, Kubick, Stefan
Publicado 2020

Cell-free synthesis of functional human epidermal growth factor receptor: Investigation of ligand-independent dimerization in Sf21 microsomal membranes using non-canonical amino ac...

por Quast, Robert B., Ballion, Biljana, Stech, Marlitt, Sonnabend, Andrei, Varga, Balázs R., Wüstenhagen, Doreen A., Kele, Péter, Schiller, Stefan M., Kubick, Stefan
Publicado 2016

Synthesis of an Anti-CD7 Recombinant Immunotoxin Based on PE24 in CHO and E. coli Cell-Free Systems

por Krebs, Simon K., Stech, Marlitt, Jorde, Felix, Rakotoarinoro, Nathanaël, Ramm, Franziska, Marinoff, Sophie, Bahrke, Sven, Danielczyk, Antje, Wüstenhagen, Doreen A., Kubick, Stefan
Publicado 2022