Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design

por Martin, Caterina, Ovalle Maqueo, Amaury, Wijma, Hein J., Fraaije, Marco W.
Publicado 2018

Stabilizing AqdC, a Pseudomonas Quinolone Signal‐Cleaving Dioxygenase from Mycobacteria, by FRESCO‐Based Protein Engineering

por Wullich, Sandra C., Wijma, Hein J., Janssen, Dick B., Fetzner, Susanne
Publicado 2020

Characterization of two bacterial multi-flavinylated proteins harboring multiple covalent flavin cofactors

por Tong, Yapei, Rozeboom, Henriette J., Loonstra, Marnix R., Wijma, Hein J., Fraaije, Marco W.
Publicado 2023

Computational Prediction of ω-Transaminase Specificity by a Combination of Docking and Molecular Dynamics Simulations

por Ramírez-Palacios, Carlos, Wijma, Hein J., Thallmair, Sebastian, Marrink, Siewert J., Janssen, Dick B.
Publicado 2021

Stabilization of cyclohexanone monooxygenase by a computationally designed disulfide bond spanning only one residue

por van Beek, Hugo L., Wijma, Hein J., Fromont, Lucie, Janssen, Dick B., Fraaije, Marco W.
Publicado 2014

A conserved sequence motif in the Escherichia coli soluble FAD-containing pyridine nucleotide transhydrogenase is important for reaction efficiency

por Partipilo, Michele, Yang, Guang, Mascotti, Maria Laura, Wijma, Hein J., Slotboom, Dirk Jan, Fraaije, Marco W.
Publicado 2022

Computationally designed libraries for rapid enzyme stabilization

por Wijma, Hein J., Floor, Robert J., Jekel, Peter A., Baker, David, Marrink, Siewert J., Janssen, Dick B.
Publicado 2014

Real-Time Conformational Changes and Controlled Orientation of Native Proteins Inside a Protein Nanoreactor

por Van Meervelt, Veerle, Soskine, Misha, Singh, Shubham, Schuurman-Wolters, Gea K., Wijma, Hein J., Poolman, Bert, Maglia, Giovanni
Publicado 2017

Computational Design of Enantiocomplementary Epoxide Hydrolases for Asymmetric Synthesis of Aliphatic and Aromatic Diols

por Arabnejad, Hesam, Bombino, Elvira, Colpa, Dana I., Jekel, Peter A., Trajkovic, Milos, Wijma, Hein J., Janssen, Dick B.
Publicado 2020

Catalytic and structural properties of ATP‐dependent caprolactamase from Pseudomonas jessenii

por Marjanovic, Antonija, Rozeboom, Henriëtte J., de Vries, Meintje S., Mayer, Clemens, Otzen, Marleen, Wijma, Hein J., Janssen, Dick B.
Publicado 2021

Hot or not? Discovery and characterization of a thermostable alditol oxidase from Acidothermus cellulolyticus 11B

por Winter, Remko T., Heuts, Dominic P. H. M., Rijpkema, Egon M. A., van Bloois, Edwin, Wijma, Hein J., Fraaije, Marco W.
Publicado 2012

Metabolism of β-valine via a CoA-dependent ammonia lyase pathway

por Otzen, Marleen, Crismaru, Ciprian G., Postema, Christiaan P., Wijma, Hein J., Heberling, Matthew M., Szymanski, Wiktor, de Wildeman, Stefaan, Janssen, Dick B.
Publicado 2015

Computation-Aided Engineering of Cytochrome P450 for the Production of Pravastatin

por Ashworth, Mark A., Bombino, Elvira, de Jong, René M., Wijma, Hein J., Janssen, Dick B., McLean, Kirsty J., Munro, Andrew W.
Publicado 2022

Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor

por Monteiro, Francisca, Hubmann, Georg, Takhaveev, Vakil, Vedelaar, Silke R, Norder, Justin, Hekelaar, Johan, Saldida, Joana, Litsios, Athanasios, Wijma, Hein J, Schmidt, Alexander, Heinemann, Matthias
Publicado 2019

CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate Modifications and Protein Engineering

por Bracco, Paula, Wijma, Hein J., Nicolai, Bastian, Buitrago, Jhon Alexander Rodriguez, Klünemann, Thomas, Vila, Agustina, Schrepfer, Patrick, Blankenfeldt, Wulf, Janssen, Dick B., Schallmey, Anett
Publicado 2020

From thiol-subtilisin to omniligase: Design and structure of a broadly applicable peptide ligase

por Toplak, Ana, Teixeira de Oliveira, Eduardo F., Schmidt, Marcel, Rozeboom, Henriëtte J., Wijma, Hein J., Meekels, Linda K.M., de Visser, Rowin, Janssen, Dick B., Nuijens, Timo
Publicado 2021

Robust ω-Transaminases by Computational Stabilization of the Subunit Interface

por Meng, Qinglong, Capra, Nikolas, Palacio, Cyntia M., Lanfranchi, Elisa, Otzen, Marleen, van Schie, Luc Z., Rozeboom, Henriëtte J., Thunnissen, Andy-Mark W. H., Wijma, Hein J., Janssen, Dick B.
Publicado 2020

Computational Redesign of an ω-Transaminase from Pseudomonas jessenii for Asymmetric Synthesis of Enantiopure Bulky Amines

por Meng, Qinglong, Ramírez-Palacios, Carlos, Capra, Nikolas, Hooghwinkel, Mattijs E., Thallmair, Sebastian, Rozeboom, Henriëtte J., Thunnissen, Andy-Mark W. H., Wijma, Hein J., Marrink, Siewert J., Janssen, Dick B.
Publicado 2021

Substrate Induced Movement of the Metal Cofactor between Active and Resting State

por Marsden, Stefan R., Wijma, Hein J., Mohr, Michael K. F., Justo, Inês, Hagedoorn, Peter‐Leon, Laustsen, Jesper, Jeffries, Cy M., Svergun, Dmitri, Mestrom, Luuk, McMillan, Duncan G. G., Bento, Isabel, Hanefeld, Ulf
Publicado 2022