Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli

por Karyolaimos, Alexandros, de Gier, Jan-Willem
Publicado 2021

Isolating Escherichia coli strains for recombinant protein production

por Schlegel, Susan, Genevaux, Pierre, de Gier, Jan-Willem
Publicado 2016

Revolutionizing membrane protein overexpression in bacteria

por Schlegel, Susan, Klepsch, Mirjam, Gialama, Dimitra, Wickström, David, Slotboom, Dirk Jan, De Gier, Jan‐Willem
Publicado 2010

Cotranslational folding of alkaline phosphatase in the periplasm of Escherichia coli

por Elfageih, Rageia, Karyolaimos, Alexandros, Kemp, Grant, de Gier, Jan‐Willem, von Heijne, Gunnar, Kudva, Renuka
Publicado 2020

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

por Schlegel, Susan, Rujas, Edurne, Ytterberg, Anders Jimmy, Zubarev, Roman A, Luirink, Joen, de Gier, Jan-Willem
Publicado 2013

Revealing the mechanisms of membrane protein export by virulence-associated bacterial secretion systems

por Krampen, Lea, Malmsheimer, Silke, Grin, Iwan, Trunk, Thomas, Lührmann, Anja, de Gier, Jan-Willem, Wagner, Samuel
Publicado 2018

High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG

por Zhang, Zhe, Kuipers, Grietje, Niemiec, Łukasz, Baumgarten, Thomas, Slotboom, Dirk Jan, de Gier, Jan-Willem, Hjelm, Anna
Publicado 2015

Influenza virus and pneumococcal neuraminidases enhance catalysis by similar yet distinct sialic acid–binding strategies

por Klenow, Laura, Elfageih, Rageia, Gao, Jin, Wan, Hongquan, Withers, Stephen G., de Gier, Jan-Willem, Daniels, Robert
Publicado 2023

The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli

por Kuipers, Grietje, Karyolaimos, Alexandros, Zhang, Zhe, Ismail, Nurzian, Trinco, Gianluca, Vikström, David, Slotboom, Dirk Jan, de Gier, Jan-Willem
Publicado 2017

Application of an E. coli signal sequence as a versatile inclusion body tag

por Jong, Wouter S. P., Vikström, David, Houben, Diane, van den Berg van Saparoea, H. Bart, de Gier, Jan-Willem, Luirink, Joen
Publicado 2017

Functional and structural characterization of an ECF-type ABC transporter for vitamin B12

por Santos, Joana A, Rempel, Stephan, Mous, Sandra TM, Pereira, Cristiane T, ter Beek, Josy, de Gier, Jan-Willem, Guskov, Albert, Slotboom, Dirk J
Publicado 2018

Enhancing Recombinant Protein Yields in the E. coli Periplasm by Combining Signal Peptide and Production Rate Screening

por Karyolaimos, Alexandros, Ampah-Korsah, Henry, Hillenaar, Tamara, Mestre Borras, Anna, Dolata, Katarzyna Magdalena, Sievers, Susanne, Riedel, Katharina, Daniels, Robert, de Gier, Jan-Willem
Publicado 2019

Mutagenesis-Based Characterization and Improvement of a Novel Inclusion Body Tag

por Jong, Wouter S. P., ten Hagen-Jongman, Corinne M., Vikström, David, Dontje, Wendy, Abdallah, Abdallah M., de Gier, Jan-Willem, Bitter, Wilbert, Luirink, Joen
Publicado 2020

Escherichia coli Can Adapt Its Protein Translocation Machinery for Enhanced Periplasmic Recombinant Protein Production

por Karyolaimos, Alexandros, Dolata, Katarzyna Magdalena, Antelo-Varela, Minia, Mestre Borras, Anna, Elfageih, Rageia, Sievers, Susanne, Becher, Dörte, Riedel, Katharina, de Gier, Jan-Willem
Publicado 2020

Bicistronic Design-Based Continuous and High-Level Membrane Protein Production in Escherichia coli

por Claassens, Nico J., Finger-Bou, Max, Scholten, Bart, Muis, Frederieke, de Groot, Jonas J., de Gier, Jan-Willem, de Vos, Willem M., van der Oost, John
Publicado 2019

Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)

por Baumgarten, Thomas, Schlegel, Susan, Wagner, Samuel, Löw, Mirjam, Eriksson, Jonas, Bonde, Ida, Herrgård, Markus J., Heipieper, Hermann J., Nørholm, Morten H. H., Slotboom, Dirk Jan, de Gier, Jan-Willem
Publicado 2017

New Approach to Privacy-Preserving Clinical Decision Support Systems for HIV Treatment

por Spini, Gabriele, Mancini, Emiliano, Attema, Thomas, Abspoel, Mark, de Gier, Jan, Fehr, Serge, Veugen, Thijs, van Heesch, Maran, Worm, Daniël, De Luca, Andrea, Cramer, Ronald, Sloot, Peter M.A.
Publicado 2022

A structurally informed autotransporter platform for efficient heterologous protein secretion and display

por Jong, Wouter SP, Soprova, Zora, de Punder, Karin, ten Hagen-Jongman, Corinne M, Wagner, Samuel, Wickström, David, de Gier, Jan-Willem, Andersen, Peter, van der Wel, Nicole N, Luirink, Joen
Publicado 2012

An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines

por Jong, Wouter SP, Daleke-Schermerhorn, Maria H, Vikström, David, ten Hagen-Jongman, Corinne M, de Punder, Karin, van der Wel, Nicole N, van de Sandt, Carolien E, Rimmelzwaan, Guus F, Follmann, Frank, Agger, Else Marie, Andersen, Peter, de Gier, Jan-Willem, Luirink, Joen
Publicado 2014