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| LEADER |
00000cam a2200000Ia 4500 |
| 001 |
ocm29518617 |
| 003 |
OCoLC |
| 005 |
20150815141110.0 |
| 008 |
050926s1994 ctuad b 00 0 eng d |
| 035 |
|
|
|a (Sirsi) i9780838506424
|
| 040 |
|
|
|a DLC
|c DLC
|d UV#
|
| 020 |
|
|
|a 0838506429
|
| 050 |
|
4 |
|a QH506
|b D384 1994
|
| 082 |
0 |
0 |
|a 574.8/8/028
|2 20
|
| 100 |
1 |
|
|a Davis, Leonard G.
|9 366133
|
| 245 |
1 |
0 |
|a Basic methods in molecular biology /
|c Leonard G. Davis, W. Michael Kuehl, James F. Battey.
|
| 250 |
|
|
|a 2nd ed.
|
| 260 |
|
|
|a Norwalk, Conn. :
|b Appleton & Lange,
|c c1994.
|
| 300 |
|
|
|a xiv, 777 p. :
|b il., tablas ;
|c 26 cm.
|
| 504 |
|
|
|a Incluye bibliografías.
|
| 505 |
0 |
|
|a General methods -- Bacterial strains and cloning vectors -- Enzymes that modify DNA and RNA -- In vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase -- DNA restriction fragment analysis and preparation -- In vitro labeling of probes and filter hybridization -- Plasmid DNA preparation from E coli hosts -- Preparation of DNA from lambda bacteriophage clones -- Subcloning fragments into plasmid vectors and plasmid construction -- Preparation of genomic DNA -- Preparation and analysis of RNA from eukaryotic cells -- Genomic cloning -- Preparation and analysis of cDNA libraries -- Preparation of subtractive cDNA -- Chain termination sequencing -- Transfection of mammalian cells -- Basic methods of protein analysis -- In situ hybridization -- Transgenic mouse preparation -- Detection and in vitro generation of specific mutations in genes and cDNAs -- Preparation of stock solutions -- Generally useful information -- Suppliers of reagents and equipment.
|
| 650 |
|
4 |
|a Biología molecular
|x Metodología.
|
| 700 |
1 |
|
|a Kuehl, W. Michael.
|9 366134
|
| 700 |
1 |
|
|a Battey, James F.
|
| 901 |
|
|
|a Z0
|b UV#
|
| 902 |
|
|
|a DGBUV
|
| 596 |
|
|
|a 13
|
| 942 |
|
|
|c LIBRO
|6 _
|
| 999 |
|
|
|c 184640
|d 184640
|
