Cargando…
Basic methods in molecular biology /
Autor principal: | |
---|---|
Otros Autores: | , |
Formato: | Libro |
Lenguaje: | English |
Publicado: |
Norwalk, Conn. :
Appleton & Lange,
c1994.
|
Edición: | 2nd ed. |
Materias: |
Tabla de Contenidos:
- General methods
- Bacterial strains and cloning vectors
- Enzymes that modify DNA and RNA
- In vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase
- DNA restriction fragment analysis and preparation
- In vitro labeling of probes and filter hybridization
- Plasmid DNA preparation from E coli hosts
- Preparation of DNA from lambda bacteriophage clones
- Subcloning fragments into plasmid vectors and plasmid construction
- Preparation of genomic DNA
- Preparation and analysis of RNA from eukaryotic cells
- Genomic cloning
- Preparation and analysis of cDNA libraries
- Preparation of subtractive cDNA
- Chain termination sequencing
- Transfection of mammalian cells
- Basic methods of protein analysis
- In situ hybridization
- Transgenic mouse preparation
- Detection and in vitro generation of specific mutations in genes and cDNAs
- Preparation of stock solutions
- Generally useful information
- Suppliers of reagents and equipment.