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Gene silencing by RNA interference : technology and application /
| Otros Autores: | |
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| Formato: | Libro |
| Lenguaje: | English |
| Publicado: |
Boca Raton, FL :
CRC Press,
c2005.
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| Materias: |
Tabla de Contenidos:
- Gene silencing by RNA interference and the role of small interfering RNAs
- Basics of siRNA design and chemical synthesis
- Oligonucleotide scanning arrays in the design of small interfering RNAs
- siRNA production by in vitro transcription
- Production of siRNAs with the application of deoxyribozymes
- Production of siRNA in vitro by enzymatic digestion of double-stranded RNA
- Plasmid-mediated intracellular expression of siRNAs
- Lentiviral vector-mediated delivery of si/shRNA
- Exogenous siRNA delivery: protocols for optimizing delivery to cells
- RNAi in drosophila cell cultures
- RNAi in caenorhabditis elegans
- Delivery of RNAi reagents in C. elegans by microinjection
- RNAi in drosophila embryos
- RNAi in xenopus laevis oocytes
- RNAi in xenopus embryos
- An RNAi-based genomic library for forward genetics in the African trypanosome
- RNAi in the malaria parasite plasmodium
- Silencing the expression of cysteine proteases (falcipains) in plasmodium falciparum using RNA interference approaches
- RNAi in transgenic animal models
- RNA interference in mouse models
- Virus-induced RNA silencing in plants
- RNA silencing in plants: biolistic delivery of RNAi reagents
- Agro-infiltration: a versatile tool for RNAi studies in plants.