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Plant epigenetics : methods and protocols /

Detalles Bibliográficos
Otros Autores: Kovalchuk, Igor, Zemp, Franz J.
Formato: Libro
Lenguaje:English
Publicado: New York : Humana Press, c2010.
Colección:Springer protocols.
Methods in molecular biology (Clifton, N.J.) ; v. 631.
Materias:
Tabla de Contenidos:
  • Analysis of DNA methylation in plants by bisulfite sequencing / Andrea Foerster and Ortrun Mittelsten Scheid
  • Analysis of bisulfite sequencing data from plant DNA using CyMATE / Andrea M. Foerster ...[et al.]
  • Analysis of locus-specific changes in methylation patterns using a COBRA (combined bisulfite restriction analysis) assay / Alex Boyko and Igor Kovalchuk
  • Detection of changes in global genome methylation using the cytosine-extension assay / Alex Boyko and Igor Kovalchuk
  • In situ analysis of DNA methylation in plants / Palak Kathiria and Igor Kovalchuk
  • Analysis of mutation/rearrangement frequencies and methylation patterns at a given DNA locus using restriction fragment length polymorphism / Alex Boyko and Igor Kovalchuk
  • Isoschizomers and amplified fragment length polymorphism for the detection of specific cytosine methylation changes / Leonor Ruiz-García ... [et al.]
  • Analysis of small RNA populations using hybridization to DNA tiling arrays / Martine Boccara ... [et al.]
  • Northern blotting techniques for small RNAs / Todd Blevins
  • qRT-PCR of small RNAs / Erika Varkonyi-Gasic and Roger P. Hellens
  • Cloning new small RNA sequences / Yuko Tagami, Naoko Inaba, and Yuichiro Watanabe
  • Genome-wide mapping of protein-DNA interaction by chromatin immunoprecipitatino and DNA microarray hybridization (ChIP-chip). Part A : ChiP-chip molecular methods / Julia J. Reimer and Franziska Turck. Part B : ChIP-chip data analysis / Ulrike Göbel, Julia Reimer, and Franziska Turck
  • Metaanalysis of ChIP-chip data / Julia Engelhorn and Franziska Turck
  • Chromatin immunoprecipitation protocol for histone modifications and protein-DNA binding analyses in Arabidopsis / Stéphane Pien and Ueli Grossniklaus
  • cDNA libraries for virus-induced gene silencing / Andrea T. Todd, Enwu Liu, and Jonathan E. Page
  • Detection and quantification of DNA strand breaks using the ROPS (random oligonucleotide primed synthesis) assay / Alex Boyko and Igor Kovalchuk
  • Reporter gene-based recombination lines for studies of genome stability / Palak Kathiria and Igor Kovalchuk
  • Plant transgenesis / Alicja Ziemienowicz.