Peptide nucleic acids : methods and protocols /

Detalles Bibliográficos
Otros Autores: Nielsen, Peter E., 1951-
Formato: Libro
Lenguaje:English
Publicado: Totowa, N.J. : Humana Press, c2002.
Colección:Methods in molecular biology (Clifton, N.J.) ; v. 208.
Materias:
ADN > Derivados.
Peptidos > Derivados.
Tabla de Contenidos:
  • Machine generated contents note: I INTRODUCTION
  • 1 PNA Technology
  • Peter E. Nielsen
  • II CHEMISTRY
  • 2 Solid Phase Synthesis of PNA Oligomers
  • Frederik Beck
  • 3 Synthesis of PNA-Peptide Conjugates
  • Satish Kumar Awasthi and Peter E. Nielsen
  • 4 Parallel Synthesis of PNA-Peptide Conjugate Libraries
  • Satish Kumar Awasthi and Peter E. Nielsen
  • 5 Thermodynamics of PNA Interactions
  • with DNA and RNA
  • Tommi Ratilainen and Bengt Norden
  • III PNA TECHNOLOGY
  • 6 Measurement of PNA Binding to Double-Stranded DNA
  • Thomas Bentin, Georg I. Hansen,
  • and Peter E. Nielsen.
  • 7 PNA-Mediated Immobilization of Supercoiled DNA
  • Thomas Bentin and Peter E. Nielsen
  • 8 PNA Openers and Their Applications
  • Vadim V. Demidov
  • and Maxim D. Frank-Kamenetskii
  • 9 Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes
  • Miaomiao Wang and Bruce A. Armitage
  • IV DIAGNOSTICS
  • 10 PNA-Mediated PCR Clamping:
  • Applications and Methods
  • Deborah G. Murdock and Douglas C. Wallace
  • 11 PNA Clamping Techniques for the Determination of Oncogene Mutations
  • Marcus Schuermann and Moira Behn
  • 12 PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic Analysis
  • Brett Williams, Henrik Stender,
  • and James M. Coull
  • 13 Detection of Point Mutations Using PNA-Containing Electrophoresis Matrices
  • Gabor L. Igloi
  • V ANTISENSE AND ANTIGENE
  • 14 Lipid-Mediated Introduction
  • of Peptide Nucleic Acids Into Cells
  • Dwaine A. Braasch and David R. Corey.
  • 15 Synthesis of Cell-Penetrating Peptide-PNA Constructs
  • Margus Pooga, Ursel Soomets, Tamas Bartfai,
  • and Ulo Langel
  • 16 Antisense Inhibition of Bacterial Gene Expression and Cell Growth
  • Liam Good
  • 17 In Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement Loops
  • Niels Erik Mollegaard and Peter E. Nielsen
  • VI ANIMAL STUDIES
  • 18 In Vitro and in Vivo Studies on Pharmacokinetics and Metabolism of PNA Constructs in Rodents
  • Edward Kristensen
  • Index.

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