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Transgenic plants : methods and protocols /

Detalles Bibliográficos
Otros Autores: Dunwell, Jim M., Wetten, Andy C.
Formato: Libro
Lenguaje:English
Publicado: New York : Humana Press ; Springer, 2012, 2012.
Edición:Second edition.
Colección:Methods in molecular biology (Clifton, N.J.) ; v. 847.
Materias:
Tabla de Contenidos:
  • Employment of cytokinin vectors for marker-free and backbone-free transformation
  • Organophosphorus hydrolase: A multifaceted plant genetic marker which is selectable, scorable, and quantifiable in whole seed
  • Use of northern blotting for specific detection of small RNA molecules in transgenic plants
  • Genetic transformation of the model green alga Chlamydomonas reinhardtii
  • A high-efficiency agrobacterium-mediated transformation system of rice (Oryza sativa L.)
  • Selection of transgenic rice plants using a herbicide tolerant form of the acetolactate synthase gene
  • Visual selection in rice: A strategy for the efficient identification of transgenic calli accumulating transgene products
  • Characterization of rice genes using a heterologous full-length cCDNA expression system
  • Bioactive bead-mediated transformation of plants with large DNA fragments
  • Agrobacterium-mediated transformation of sorghum bicolor using immature embryos
  • Split-transgene expression in wheat
  • Agrobacterium-mediated transformation of brachypodium distachyon
  • Transformation of barley (Hordeum vulgare L.) by agrobacterium tumefaciens infection of in vitro cultured ovules
  • Biolistic-mediated production of transgenic oil palm
  • Transformation of oil palm using agrobacterium tumefaciens
  • Highly efficient transformation protocol for plum (Prunus domestica L.)
  • Co-transformation of grapevine somatic embryos to produce transgenic plants free of marker genes
  • Initiation and transformation of grapevine embryogenic cultures
  • Development of highly efficient genetic transformation protocols for table grape sugraone and crimson seedless
  • Cotton pistil drip transformation method
  • Enhanced agrobacterium-mediated transformation of embryogenic calli of upland cotton
  • Targeted biolistics for improved transformation of impatiens balsamina
  • A protocol for transformation of torenia
  • Efficient modification of floral traits by heavy-ion beam irradiation on transgenic torenia
  • Expression of artificial micrornas in physcomitrella patens
  • High frequency of single-copy t-DNA transformants produced after floral dip in CRE-expressing arabidopsis plants
  • A developmentally regulated cre-lox system to generate marker-free transgenic Brassica napus plants
  • Exploiting multisite gateway and pENFRUIT plasmid collection for fruit genetic engineering
  • A one-time inducible transposon to create knockout mutants in rice
  • Marker-free gene targeting by recombinase-mediated cassette exchange
  • Targeting DNA to a previously integrated transgenic locus using zinc finger nucleases
  • Double-strand break-induced targeted mutagenesis in plants
  • Combinatorial genetic transformation of cereals and the creation of metabolic libraries for the carotenoid pathway
  • Production of a his-tagged canecystatin in transgenic sugarcane
  • Plastid transformation as an expression tool for plant-derived biopharmaceuticals
  • Use of a callus-specific selection system to develop transgenic rice seed accumulating a high level of recombinant protein
  • How to grow transgenic arabidopsis in the field.