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|a Sambrook, Joseph
|9 367222
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| 245 |
1 |
4 |
|a The condensed protocols from Molecular cloning : a laboratory manual
|c / Joseph Sambrook, David W. Russell.
|
| 260 |
|
|
|a Cold Spring Harbor, N.Y. :
|b Cold Spring Harbor Laboratory Press,
|c ©2006.
|
| 300 |
|
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|a v, 800 páginas :
|b ilustraciones ;
|c 28 cm.
|
| 504 |
|
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|a Incluye bibliografías e índice.
|
| 505 |
0 |
|
|a Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
|
| 650 |
|
4 |
|a Clonación molecular
|v Manuales de laboratorio
|9 361484
|
| 700 |
1 |
|
|a Russell, David W.
|q (David William),
|d 1957-
|9 379477
|
| 700 |
1 |
|
|a Sambrook, Joseph.
|t Molecular cloning.
|
| 856 |
4 |
1 |
|u http://www.loc.gov/catdir/toc/ecip0517/2005022077.html
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