The condensed protocols from Molecular cloning : a laboratory manual

Detalles Bibliográficos
Autor principal: Sambrook, Joseph
Otros Autores: Russell, David W. (David William), 1957-
Formato: Libro
Lenguaje:English
Publicado: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, ©2006.
Materias:
Clonación molecular > Manuales de laboratorio
Acceso en línea:http://www.loc.gov/catdir/toc/ecip0517/2005022077.html
Tabla de Contenidos:
  • Plasmids and their usefulness in molecular cloning
  • Bacteriophage and its vectors
  • Working with bacteriophage M13 vectors
  • Working with high-capacity vectors
  • Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis
  • Preparation and analysis of eukaryotic genomic DNA
  • Extraction, purification, and analysis of mRNA from eukaryotic cells
  • In vitro amplification of DNA by the polymerase chain reaction
  • Preparation of radiolabeled DNA and RNA probes
  • Working with synthetic oligonucleotide probes
  • Preparation of cDNA libraries and gene identification
  • DNA sequencing
  • Mutagenesis
  • Screening expression libraries
  • Expression of cloned genes in escherichia coli
  • Introducing cloned genes into cultured mammalian cells
  • Analysis of gene expression in cultured mammalian cells
  • Protein interaction technologies.

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