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Immunocytochemical methods and protocols
| Otros Autores: | |
|---|---|
| Formato: | Libro |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press,
[1999]
|
| Edición: | 2nd ed. |
| Colección: | Methods in molecular biology ;
v. 115 |
| Materias: |
Tabla de Contenidos:
- Part I. Antibody Preparation
- Overview of Antibody Use in Immunocytochemistry / Su-Yau Mao, Lorette C. Javois, and Ute M. Kent
- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration / Ute M. Kent
- Purification of Antibodies Using Ion-Exchange Chromatography / Ute M. Kent
- Purification of Antibodies Using Affinity Chromatography / Ute M. Kent
- Purification of Antibodies Using Protein A-Sepharose and FPLC / Ute M. Kent
- Conjugation of Fluorochromes to Antibodies / Su-Yau Mao
- Biotinylation of Antibodies / Su-Yau Mao
- Part II. Tissue Preparation for light Microscopic Analyses
- Overview of Cell Fixatives and Cell Membrane Permeants / Melissa A. Melan
- Preparation of Frozen Sections for Analysis / Gary L. Bratthauer
- Processing of Cytological Specimens / Gary L. Bratthauer
- Processing of Tissue Culture Cells / Gary L. Bratthauer
- Processing of Tissue Specimens / Gary L. Bratthauer
- Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections / Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld
- Part III. Light Microscopic Detection Systems
- Overview of Fluorochromes / J. Michael Mullins
- Direct Immunofluorescent Labeling of Cells / Lorette C. Javois
- Fluorescence Labeling of Surface Antigens on Attached or Suspended Tissue-Culture Cells / Mark C. Willingham
- Fluorescence Labeling of Intracellular Antigens in Attached or Suspended Tissue-Culture Cells / Mark C. Willingham
- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts / Lorette C. Javois
- TUNEL Assay for Apoptotic Cells / Virginia M. Heatwole
- Overview of Fluorescence Analysis with the Confocal Microscope / Liana Harvath
- Overview of Laser Microbeam Applications as Related to Antibody Targeting / P. Scott Pine
- Overview of Fluorescence Photomicography / Lorette C. Javois and J. Michael Mullins
- Overview of Antigen Detection Through Enzymatic Activity / Gary L. Bratthauer
- The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods / Gary L. Bratthauer
- The Avidin Biotin Complex (ABC) Method and Other Avidin Biotin Binding Methods / Gary L. Bratthauer
- Avidin Biotin Labeling of Cellular Antigens in Cryostat Sectioned Tissue / Mark Raffeld and Elaine S. Jaffe
- Multiple Antigen Immunostaining Procedures / Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld
- ELISA on Attachment Dependent or Suspension Grown Cells / Julie Ann Brent
- Use of Immunogold with Silver Enhancement / Constance Oliver
- Part IV. Fluorescence Activated Cell Sorter (FACS) Analyses
- Overview of Flow Cytometry and Fluorescent Probes for Cytometry / Robert E. Cunningham
- Tissue Disaggregation/ Robert E. Cunningham
- Indirect Immunofluorescent Labeling of Viable Cells / Robert E. Cunningham
- Indirect Immunofluorescent Labeling of Fixed Cells / Robert E. Cunningham
- Fluorescent Labeling of DNA / Robert E. Cunningham
- Deparaffinization and Processing of Pathologic Material / Robert E. Cunningham
- Assay for Phagocytosis / Liana Harvath and Douglas A. Terle
- Assay for Filamentous Actin / Liana Harvath
- Assay for Chemoattractant Binding / Liana Harvath, Robert R. Aksamit, and Robert E. Cunningham
- Assay for Oxidative Metabolism / Liana Harvath and Douglas A. Terle
- Part V. Colloidal Gold Detection Systems For Electron Microscopic Analyses
- Fixation and Embedding / Constance Oliver and Maria Celia Jamur
- Preparation of Colloidal Gold / Constance Oliver
- Conjugation of Colloidal Gold to Proteins / Constance Oliver
- Colloidal Gold/Streptavidin Methods / Constance Oliver
- Pre-Embedding Labeling Methods / Constance Oliver
- Postembedding Labeling Methods / Constance Oliver
- Part VI. Applications For Nucleic Acid Localization By In Situ Hybridization
- Overview of In Situ Hybridization / Roland M. Nardone
- In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe / Roland M. Nardone
- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes / Roland M. Nardone
- In Situ RT-PCR and Hybridization Techniques / Vishakha Thaker
- Part VII. Special Applications For the Clinical Laboratory
- Overview of the Clinical Immunohistochemistry Laboratory : Regulations and Troubleshooting Guidelines / Patricia A. Fetsch and Andrea Abati
- Overview of Immunohistochemical Approaches to the Differential Diagnosis of Tumors / Dennis M. Frisman
- Overview of Automated Immunostainers / Gilbert E. Herman, Edna A. Elfont, and Alton D. Floyd.