|
|
|
|
| LEADER |
00000cam a2200000 i 4500 |
| 001 |
ocn774021237 |
| 003 |
UV# |
| 005 |
20171030094153.0 |
| 008 |
160609s2012 nyua b 001 0 eng d |
| 999 |
|
|
|c 299694
|d 299694
|
| 010 |
|
|
|a 2012002613
|
| 020 |
|
|
|a 9781936113415
|q (pasta dura)
|
| 020 |
|
|
|a 1936113414
|q (pasta dura)
|
| 020 |
|
|
|a 9781936113422
|q (rústica)
|
| 020 |
|
|
|a 1936113422
|q (rústica)
|
| 040 |
|
|
|a DLC
|b spa
|c DLC
|d UV#
|
| 050 |
0 |
4 |
|a QH442.2
|b G73 2012
|
| 082 |
0 |
0 |
|a 572.8
|2 23
|
| 100 |
1 |
|
|a Green, Michael R.
|q (Michael Richard),
|d 1954-
|9 427681
|
| 245 |
1 |
0 |
|a Molecular cloning :
|b a laboratory manual /
|c Michael R. Green, Joseph Sambrook.
|
| 250 |
|
|
|a Fourth edition.
|
| 264 |
|
1 |
|a Cold Spring Harbor, New York :
|b Cold Spring Harbor Laboratory Press,
|c [2012].
|
| 264 |
|
4 |
|c ©2012
|
| 300 |
|
|
|a 3 volúmenes (xxxiii, 1890, I-46 páginas) :
|b ilustraciones (algunas en color) ;
|c 29 cm.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a unmediated
|b n
|2 rdamedia
|
| 338 |
|
|
|a volume
|b nc
|2 rdacarrier
|
| 504 |
|
|
|a Incluye bibliografías e índice.
|
| 505 |
0 |
|
|a v. 1. Part 1: Essentials. Isolation and quantification of DNA ; Analysis of DNA ; Cloning and transformation with plasmid vectors ; Gateway recombinational cloning ; Working with bacterial artificial chromosomes and other high-capacity vectors ; Extraction, purification, and analysis of RNA from eukaryotic cells ; Polymerase chain reaction ; Bioinformatics -- v. 2. Part 2: Analysis and manipulation of DNA and RNA. Quantification of DNA and RNA by real-time polymerase chain reactions ; Nucleic acid platform technologies ; DNA sequencing ; Analysis of labeled DNA, RNA, and oligonucleotide probes ; Methods for vitro mutagenesis. Part 3: Introducing genes into cells. Introducing genes into cultured mammalian cells ; Introducing genes into mammalian cells : viral vectors -- v. 3. Part 4: Gene expression. Analysis of gene regulation using reporter systems ; RNA interference and small RNA analysis ; Expressing cloned genes for protein production, purification, and analysis. Part 5: Interaction analysis. Cross-linking technologies for analysis of chromatin structure and function ; Mapping of in vivo RNA-binding sites by UV-cross-linking immunoprecipitation (CLIP). Appendices. Reagents and buffers ; Commonly used techniques ; Detection systems ; General safety and hazardous material.
|
| 650 |
|
4 |
|a Clonación molecular
|v Manuales de laboratorio
|9 361484
|
| 700 |
1 |
|
|a Sambrook, Joseph
|9 367222
|
| 942 |
|
|
|c LIBRO
|2 lcc
|6 _
|
