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Molecular cloning : a laboratory manual /
| Autor principal: | |
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| Otros Autores: | |
| Formato: | Libro |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor, New York :
Cold Spring Harbor Laboratory Press,
[2012].
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| Edición: | Fourth edition. |
| Materias: |
Tabla de Contenidos:
- v. 1. Part 1: Essentials. Isolation and quantification of DNA ; Analysis of DNA ; Cloning and transformation with plasmid vectors ; Gateway recombinational cloning ; Working with bacterial artificial chromosomes and other high-capacity vectors ; Extraction, purification, and analysis of RNA from eukaryotic cells ; Polymerase chain reaction ; Bioinformatics
- v. 2. Part 2: Analysis and manipulation of DNA and RNA. Quantification of DNA and RNA by real-time polymerase chain reactions ; Nucleic acid platform technologies ; DNA sequencing ; Analysis of labeled DNA, RNA, and oligonucleotide probes ; Methods for vitro mutagenesis. Part 3: Introducing genes into cells. Introducing genes into cultured mammalian cells ; Introducing genes into mammalian cells : viral vectors
- v. 3. Part 4: Gene expression. Analysis of gene regulation using reporter systems ; RNA interference and small RNA analysis ; Expressing cloned genes for protein production, purification, and analysis. Part 5: Interaction analysis. Cross-linking technologies for analysis of chromatin structure and function ; Mapping of in vivo RNA-binding sites by UV-cross-linking immunoprecipitation (CLIP). Appendices. Reagents and buffers ; Commonly used techniques ; Detection systems ; General safety and hazardous material.