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Neural development : methods and protocols /

Detalles Bibliográficos
Otros Autores: Zhou, Renping (Editor ), Mei, Lin (Editor )
Formato: Libro
Lenguaje:English
Publicado: New York : Humana Press, ©2013
Colección:Methods in molecular biology (Clifton, N.J.) ; v. 1018.
Springer protocols (Series)
Materias:
Acceso en línea:http://swbplus.bsz-bw.de/bsz391107437inh.htm
Tabla de Contenidos:
  • Isolation and maintenance of cortical neural progenitor cells in vitro / Mi-Yeon Kim, Byoung-San Moon, and Kang-Yell Choi
  • Neural induction with a dopaminergic phenotype from human pluripotent stem cells through a feeder-free floating aggregation culture / Asuka Morizane, Daisuke Doi, and Jun Takahashi
  • Nucleoside analog labeling of neural stem cells and their progeny / Erik Michael DeBoer and Mladen-Roko Rasin
  • Culture of dissociated hippocampal neurons / Yun Peng, Wen-Cheng Xiong, and Lin Mei
  • Culturing mouse cerebellar granule neurons / Tharakeswari Selvakumar and Daniel L. Kilpatrick
  • Preparation of primary cultured dopaminergic neurons from mouse nrain / Won-Seok Choi, Hyung-Wook Kim, and Zhengui Xia
  • Culturing astrocytes from postnatal rats / Yangyang Huang and Cheryl F. Dreyfus
  • Culturing oligodendrocyte lineage cells from neonatal rats / Clifton G. Fulmer and Cheryl F. Dreyfus
  • Isolation and culture of schwann sells / Yanmei Tao
  • DNA transfection : calcium phosphate method / Munjin Kwon and Bonnie L. Firestein
  • Gene-gun approach for transfection and labeling of cells in brain slices / Anna Dunaevsky
  • Lentiviral vector production, titration, and transduction of primary neurons / Baojin Ding and Daniel L. Kilpatrick
  • In ovo electroporation in embryonic chick spinal cords / Hui Wang and Michael P. Matise
  • Gene transfer in developing chick embryos : in ovo electroporation / Emma K. Farley
  • In utero electroporation in mice / Chunlei Wang and Lin Mei
  • General introduction to in situ hybridization protocol using nonradioactively labeled probes to detect mRNAs on tissue sections / Daehoon Lee, Shan Xiong, and Wen-Cheng Xiong
  • Immunofluorescence staining with frozen mouse or chick embryonic tissue sections / Hui Wang and Michael P. Matise
  • [beta]-galactosidase staining of LacZ fusion proteins in whole tissue preparations / Margaret A. Cooper and Renping Zhou
  • Chromatin immunoprecipitation assay of brain tissues using percoll gradient-purified nuclei / Baojin Ding and Daniel L. Kilpatrick
  • In vivo dual luciferase reporter assay with chick neural tube in ovo electroporation system / Hui Wang and Michael P. Matise
  • Growth cone collapse assay / Xin Yue, Alexander I. Son, and Renping Zhou
  • Stripe assay : studying growth preference and axon guidance on binary choice substrates in vitro / Markus Weschenfelder ... [et al.]
  • Microcontact printing of substrate-bound protein patterns for cell and tissue culture / Martin Fritz and Martin Bastmeyer
  • Semiautomated analysis of dendrite morphology in cell culture / Eric S. Sweet ... [et al.]
  • Monitoring synaptic plasticity by imaging AMPA receptor content and dynamics on dendritic spines / Hiroshi Makino and Bo Li
  • Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms / Haitao Wu and Lin Mei
  • Routine histology techniques for the developing and adult central nervous system / W. Geoffrey McAuliffe
  • Cryosectioning / Alexander I. Son, Katie Sokolowski, and Renping Zhou
  • Golgi-cox method / Gitanjali Das, Kenneth Reuhl, and Renping Zhou
  • Neuroanatomical tract-tracing using biotinylated dextran amine / Nikolai E. Lazarov
  • Retrograde tracing technique for neonatal animals / Kengo Funakoshi, Akira Yoshikawa, and Yoshitoshi Atobe.