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Search for baculoviruses in sawflies (Hymenoptera: Diprionidae) in Mexico
Introduction: Sawflies (Hymenoptera: Diprionidae) affect conifers and other forest species in the northern hemisphere, reducing forest productivity and causing stand death, thereby justifying control actions.Objective: The aim was to collect baculoviral strains from Mexican sawfly populations and ex...
Autores principales: | , , , , , , |
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Formato: | Online Artículo |
Lenguaje: | spa |
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Universidad Autónoma Chapingo
2022
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Materias: | |
Acceso en línea: | https://revistas.chapingo.mx/forestales/article/view/r.rchscfa.2022.04.031 https://dx.doi.org/10.5154/r.rchscfa.2022.04.031 |
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author | Estrada-Emigdio, Cristian Macario-Tovar, Beatriz S. Miranda-Miranda, Estefan Cossio-Bayugar, Raquel González-Gaona, Ernesto De Lira-Ramos, Karla V. Pérez-Panduro, Alejandro |
author_facet | Estrada-Emigdio, Cristian Macario-Tovar, Beatriz S. Miranda-Miranda, Estefan Cossio-Bayugar, Raquel González-Gaona, Ernesto De Lira-Ramos, Karla V. Pérez-Panduro, Alejandro |
author_sort | Estrada-Emigdio, Cristian |
collection | Revista Chapingo Serie Ciencias Forestales y del Ambiente |
description | Introduction: Sawflies (Hymenoptera: Diprionidae) affect conifers and other forest species in the northern hemisphere, reducing forest productivity and causing stand death, thereby justifying control actions.Objective: The aim was to collect baculoviral strains from Mexican sawfly populations and explore their potential for developing biopesticides.Materials and methods: A search for baculovirus-infected field populations was carried out. Twenty-three samples of live or dead larvae were collected from 12 localities. Microscopic diagnosis at 400X or 1000X under phase contrast, a DNA hybridization test, and a pathogenicity test were performed.Results and discussion: Samples from eleven localities showed baculoviral polyhedra. Three subsamples of Zadiprion ojedae DNA from Guachochi, Chihuahua, hybridized with a synthetic probe of the Neodiprion sertifer Polh gene sequence, confirming they harbored baculovirus infection.Five tested inocula produced disease and mortality in all treated larvae; two of them induced baculoviral polyhedra proliferation in ≥ 89 % of the resulting cadavers. The rictus mortem of sawfly larvae killed by baculovirus differs from that observed in Lepidoptera: most baculovirus-bearing cadavers remain firm or hard, flattened or not, obscured or not, and do not hang from the hind legs. For most of the cadavers found with liquefied tissues, the agent that most probably caused death was not the baculovirus but the accompanying microbiota (bacilli, cocci, or fungi).Conclusions: Baculovirus strains are widely present in Mexican sawfly populations and several of them were pathogenic and promising for developing bioinsecticides. |
format | Online Article |
id | oai_chapingo-forestales-_article-11 |
institution | Universidad Autónoma Chapingo |
language | spa |
publishDate | 2022 |
publisher | Universidad Autónoma Chapingo |
record_format | ojs |
spelling | oai_chapingo-forestales-_article-112023-08-28T16:31:34Z Search for baculoviruses in sawflies (Hymenoptera: Diprionidae) in Mexico Búsqueda de baculovirus en moscas sierra (Hymenoptera: Diprionidae) en México Estrada-Emigdio, Cristian Macario-Tovar, Beatriz S. Miranda-Miranda, Estefan Cossio-Bayugar, Raquel González-Gaona, Ernesto De Lira-Ramos, Karla V. Pérez-Panduro, Alejandro Diprionidae Zadiprion Neodiprion Monoctenus bioinsecticidas Diprionidae Zadiprion Neodiprion Monocteus bioinsecticides The first search for baculoviruses in sawflies is presented. Ten populations of eight sawfly species of Mexico yielded eleven samples baculoviral inocula. A hybridization test confirmed the presence of baculoviral DNA in our samples. Two of the five tested baculoviral inocula exhibited high potential as bioinsecticides. Rictus mortem of sawflies infected with the baculovirus differs from that of lepidopterans. Introduction: Sawflies (Hymenoptera: Diprionidae) affect conifers and other forest species in the northern hemisphere, reducing forest productivity and causing stand death, thereby justifying control actions.Objective: The aim was to collect baculoviral strains from Mexican sawfly populations and explore their potential for developing biopesticides.Materials and methods: A search for baculovirus-infected field populations was carried out. Twenty-three samples of live or dead larvae were collected from 12 localities. Microscopic diagnosis at 400X or 1000X under phase contrast, a DNA hybridization test, and a pathogenicity test were performed.Results and discussion: Samples from eleven localities showed baculoviral polyhedra. Three subsamples of Zadiprion ojedae DNA from Guachochi, Chihuahua, hybridized with a synthetic probe of the Neodiprion sertifer Polh gene sequence, confirming they harbored baculovirus infection.Five tested inocula produced disease and mortality in all treated larvae; two of them induced baculoviral polyhedra proliferation in ≥ 89 % of the resulting cadavers. The rictus mortem of sawfly larvae killed by baculovirus differs from that observed in Lepidoptera: most baculovirus-bearing cadavers remain firm or hard, flattened or not, obscured or not, and do not hang from the hind legs. For most of the cadavers found with liquefied tissues, the agent that most probably caused death was not the baculovirus but the accompanying microbiota (bacilli, cocci, or fungi).Conclusions: Baculovirus strains are widely present in Mexican sawfly populations and several of them were pathogenic and promising for developing bioinsecticides. Introducción: Las moscas sierra (Hym. Diprionidae) afectan las coníferas y otras especies forestales en el hemisferio norte, reduciendo la productividad del bosque y causando la muerte de los rodales, lo que justifica acciones de control.Objetivo: Obtener cepas baculovíricas de poblaciones de moscas sierra mexicanas y explorar su potencial para elaborar bioplaguicidas.Materiales y métodos: Se hizo una búsqueda de poblaciones de campo infectadas con baculovirus. De 12 localidades se recolectaron 23 muestras de larvas vivas o muertas. Se realizó un diagnóstico microscópico a 400X o 1000X bajo contraste de fases, una prueba de hibridación de ADN y otra de patogenicidad. Resultados y discusión: Las muestras procedentes de once localidades mostraron poliedros baculovíricos. Tres submuestras de ADN de Zadiprion ojedae de Guachochi, Chihuahua, hibridaron con una sonda sintética de la secuencia del gen Polh de Neodiprion sertifer, confirmando que albergaban la infección baculovírica. Cinco inóculos probados produjeron enfermedad y mortalidad en todas las larvas tratadas; dos de ellos indujeron proliferación de poliedros baculovíricos en ≥89 % de los cadáveres resultantes. El rictus mortem de las larvas de moscas sierra muertas por baculovirus difiere del observado en los lepidópteros: la mayoría de los cadáveres portadores de baculovirus permanecen firmes o duros, aplanados o no, obscurecidos o no y no cuelgan de las patas traseras. La licuefacción de sus tejidos internos, su aplanamiento y obscurecimiento dependen de la abundancia relativa y tipo de microbiota acompañante. En la mayoría de los cadáveres encontrados con tejidos licuados, el agente que más probablemente causó la muerte no fue el baculovirus, sino la microbiota acompañante (bacilos, cocos y hongos).Conclusiones: Las cepas baculovíricas están ampliamente presentes en las poblaciones mexicanas de moscas sierra y varias de ellas fueron patogénicas y prometedoras para el desarrollo de bioinsecticidas. Universidad Autónoma Chapingo 2022-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.chapingo.mx/forestales/article/view/r.rchscfa.2022.04.031 10.5154/r.rchscfa.2022.04.031 Revista Chapingo Serie Ciencias Forestales y del Ambiente; Vol. 29 No. 1 (2023): January-April; 163-182 Revista Chapingo Serie Ciencias Forestales y del Ambiente; Vol. 29 Núm. 1 (2023): enero-abril; 163-182 2007-4018 2007-3828 spa https://revistas.chapingo.mx/forestales/article/view/r.rchscfa.2022.04.031/r.rchscfa.2022.04.031 Derechos de autor 2022 Revista Chapingo Serie Ciencias Forestales y del Ambiente https://creativecommons.org/licenses/by-nc/4.0 |
spellingShingle | Diprionidae Zadiprion Neodiprion Monoctenus bioinsecticidas Diprionidae Zadiprion Neodiprion Monocteus bioinsecticides The first search for baculoviruses in sawflies is presented. Ten populations of eight sawfly species of Mexico yielded eleven samples baculoviral inocula. A hybridization test confirmed the presence of baculoviral DNA in our samples. Two of the five tested baculoviral inocula exhibited high potential as bioinsecticides. Rictus mortem of sawflies infected with the baculovirus differs from that of lepidopterans. Estrada-Emigdio, Cristian Macario-Tovar, Beatriz S. Miranda-Miranda, Estefan Cossio-Bayugar, Raquel González-Gaona, Ernesto De Lira-Ramos, Karla V. Pérez-Panduro, Alejandro Search for baculoviruses in sawflies (Hymenoptera: Diprionidae) in Mexico |
title | Search for baculoviruses in sawflies (Hymenoptera: Diprionidae) in Mexico |
title_alt | Búsqueda de baculovirus en moscas sierra (Hymenoptera: Diprionidae) en México |
title_full | Search for baculoviruses in sawflies (Hymenoptera: Diprionidae) in Mexico |
title_fullStr | Search for baculoviruses in sawflies (Hymenoptera: Diprionidae) in Mexico |
title_full_unstemmed | Search for baculoviruses in sawflies (Hymenoptera: Diprionidae) in Mexico |
title_short | Search for baculoviruses in sawflies (Hymenoptera: Diprionidae) in Mexico |
title_sort | search for baculoviruses in sawflies (hymenoptera: diprionidae) in mexico |
topic | Diprionidae Zadiprion Neodiprion Monoctenus bioinsecticidas Diprionidae Zadiprion Neodiprion Monocteus bioinsecticides The first search for baculoviruses in sawflies is presented. Ten populations of eight sawfly species of Mexico yielded eleven samples baculoviral inocula. A hybridization test confirmed the presence of baculoviral DNA in our samples. Two of the five tested baculoviral inocula exhibited high potential as bioinsecticides. Rictus mortem of sawflies infected with the baculovirus differs from that of lepidopterans. |
topic_facet | Diprionidae Zadiprion Neodiprion Monoctenus bioinsecticidas Diprionidae Zadiprion Neodiprion Monocteus bioinsecticides The first search for baculoviruses in sawflies is presented. Ten populations of eight sawfly species of Mexico yielded eleven samples baculoviral inocula. A hybridization test confirmed the presence of baculoviral DNA in our samples. Two of the five tested baculoviral inocula exhibited high potential as bioinsecticides. Rictus mortem of sawflies infected with the baculovirus differs from that of lepidopterans. |
url | https://revistas.chapingo.mx/forestales/article/view/r.rchscfa.2022.04.031 https://dx.doi.org/10.5154/r.rchscfa.2022.04.031 |
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