Hepatitis E Virus (HEV) Spreads from Pigs and Sheep in Mongolia

SIMPLE SUMMARY: Hepatitis E virus (HEV) is a zoonotic pathogen, with an increasing number of cases worldwide. In Asia, including Mongolia, infections are associated with the zoonotic HEV-3 and HEV-4 genotypes, and pigs, deer, and wild boars are the main reservoirs. Recent studies have revealed that sheep are hosts of the virus in several countries. The aim of our study is to diagnose HEV RNA in feces and liver samples of sheep in Mongolia and clarify the origin of the virus and characterize its chain of infection. From our results, we found HEV genotype 4 in sheep and it was closely related to pig HEV genotype 4 in the same region. On Mongolian pig farms, pigs are fed with the raw internal organs of sheep for fattening the pigs as a free resource of protein. There is a concern that the spread of HEV could affect livestock feeding. ABSTRACT: Hepatitis E is a viral infectious disease in pigs, wild boars, cows, deer, rabbits, camels, and humans as hosts caused by Paslahepevirus. Recently, it has been detected in a wide variety of animals including domestic small ruminants. Mongolia is a land of nomadic people living with livestock such as sheep, goats, and cattle. Due to how Mongolian lifestyles have changed, pork has become popular and swine diseases have emerged. Among them, Hepatitis E disease has become a zoonotic infectious disease that needs to be addressed. The HEV problem in pigs is that infected pigs excrete the virus without showing clinical symptoms and it spreads into the environment. We attempted to detect HEV RNA in sheep which had been raised in Mongolia for a long time, and those animals living together with pigs in the same region currently. We also conducted a longitudinal analysis of HEV infection in pigs in the same area and found that they were infected with HEV of the same genotype and cluster. In this study, we examined 400 feces and 120 livers (pigs and sheep) by RT-PCR in Töv Province, Mongolia. HEV detection in fecal samples was 2% (4/200) in sheep and 15% (30/200) in pigs. The results of ORF2 sequence analysis of the HEV RT-PCR-positive pigs and sheep confirmed genotype 4 in both animals. The results suggest that HEV infection is widespread in both pigs and sheep and that urgent measures to prevent infection are needed. This case study points to the changing nature of infectious diseases associated with livestock farming. It will be necessary to reconsider livestock husbandry and public health issues based on these cases.