Molecular Cytogenetics in Domestic Bovids: A Review

SIMPLE SUMMARY: Molecular cytogenetics, and particularly the use of fluorescence in situ hybridization (FISH), has allowed deeper investigation of the chromosomes of domestic animals in order to: (a) create physical maps of specific DNA sequences on chromosome regions; (b) use specific chromosome markers to confirm the identification of chromosomes or chromosome regions involved in chromosome abnormalities, especially when poor banding patterns are produced; (c) better anchor radiation hybrid and genetic maps to specific chromosome regions; (d) better compare related and unrelated species by comparative FISH mapping and/or Zoo-FISH techniques; (e) study meiotic segregation, especially by sperm-FISH, in some chromosome abnormalities; (f) better show conserved or lost DNA sequences in chromosome abnormalities; (g) use informatic and genomic reconstructions, in addition to CGH arrays in related species, to predict conserved or lost chromosome regions; and (h) study some chromosome abnormalities and genomic stability using PCR applications. This review summarizes the most important applications of molecular cytogenetics in domestic bovids, with an emphasis on FISH mapping applications. ABSTRACT: The discovery of the Robertsonian translocation (rob) involving cattle chromosomes 1 and 29 and the demonstration of its deleterious effects on fertility focused the interest of many scientific groups on using chromosome banding techniques to reveal chromosome abnormalities and verify their effects on fertility in domestic animals. At the same time, comparative banding studies among various species of domestic or wild animals were found useful for delineating chromosome evolution among species. The advent of molecular cytogenetics, particularly the use of fluorescence in situ hybridization (FISH), has allowed a deeper investigation of the chromosomes of domestic animals through: (a) the physical mapping of specific DNA sequences on chromosome regions; (b) the use of specific chromosome markers for the identification of the chromosomes or chromosome regions involved in chromosome abnormalities, especially when poor banding patterns are produced; (c) better anchoring of radiation hybrid and genetic maps to specific chromosome regions; (d) better comparisons of related and unrelated species by comparative FISH mapping and/or Zoo-FISH techniques; (e) the study of meiotic segregation, especially by sperm-FISH, in some chromosome abnormalities; (f) better demonstration of conserved or lost DNA sequences in chromosome abnormalities; (g) the use of informatic and genomic reconstructions, in addition to CGH arrays, to predict conserved or lost chromosome regions in related species; and (h) the study of some chromosome abnormalities and genomic stability using PCR applications. This review summarizes the most important applications of molecular cytogenetics in domestic bovids, with an emphasis on FISH mapping applications.