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Phyto-Assisted Synthesis of Nanoselenium–Surface Modification and Stabilization by Polyphenols and Pectins Derived from Agricultural Wastes

Raw and purified mandarin peel-derived pectins were characterized and combined with olive pomace extract (OPE) in the green synthesis of selenium nanoparticles (SeNPs). SeNPs were characterized in terms of size distribution and zeta potential, and their stability was monitored during 30 days of stor...

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Autores principales: Golub, Nikolina, Galić, Emerik, Radić, Kristina, Jagodić, Ana-Maria, Predović, Nela, Katelan, Kristina, Tesla, Lucija, Pedisić, Sandra, Vinković, Tomislav, Vitali Čepo, Dubravka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000393/
https://www.ncbi.nlm.nih.gov/pubmed/36900634
http://dx.doi.org/10.3390/foods12051117
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author Golub, Nikolina
Galić, Emerik
Radić, Kristina
Jagodić, Ana-Maria
Predović, Nela
Katelan, Kristina
Tesla, Lucija
Pedisić, Sandra
Vinković, Tomislav
Vitali Čepo, Dubravka
author_facet Golub, Nikolina
Galić, Emerik
Radić, Kristina
Jagodić, Ana-Maria
Predović, Nela
Katelan, Kristina
Tesla, Lucija
Pedisić, Sandra
Vinković, Tomislav
Vitali Čepo, Dubravka
author_sort Golub, Nikolina
collection PubMed
description Raw and purified mandarin peel-derived pectins were characterized and combined with olive pomace extract (OPE) in the green synthesis of selenium nanoparticles (SeNPs). SeNPs were characterized in terms of size distribution and zeta potential, and their stability was monitored during 30 days of storage. HepG2 and Caco-2 cell models were used for the assessment of biocompatibility, while antioxidant activity was investigated by the combination of chemical and cellular-based assays. SeNP average diameters ranged from 171.3 nm up to 216.9 nm; smaller SeNPs were obtained by the utilization of purified pectins, and functionalization with OPE slightly increased the average. At concentrations of 15 mg/L SeNPs were found to be biocompatible, and their toxicity was significantly lower in comparison to inorganic selenium forms. Functionalization of SeNPs with OPE increased their antioxidant activity in chemical models. The effect was not clear in cell-based models, even though all investigated SeNPs improved cell viability and protected intracellular reduced GSH under induced oxidative stress conditions in both investigated cell lines. Exposure of cell lines to SeNPs did not prevent ROS formation after exposure to prooxidant, probably due to low transepithelial permeability. Future studies should focus on further improving the bioavailability/permeability of SeNPs and enhancing the utilization of easily available secondary raw materials in the process of phyto-mediated SeNP synthesis.
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spelling pubmed-100003932023-03-11 Phyto-Assisted Synthesis of Nanoselenium–Surface Modification and Stabilization by Polyphenols and Pectins Derived from Agricultural Wastes Golub, Nikolina Galić, Emerik Radić, Kristina Jagodić, Ana-Maria Predović, Nela Katelan, Kristina Tesla, Lucija Pedisić, Sandra Vinković, Tomislav Vitali Čepo, Dubravka Foods Article Raw and purified mandarin peel-derived pectins were characterized and combined with olive pomace extract (OPE) in the green synthesis of selenium nanoparticles (SeNPs). SeNPs were characterized in terms of size distribution and zeta potential, and their stability was monitored during 30 days of storage. HepG2 and Caco-2 cell models were used for the assessment of biocompatibility, while antioxidant activity was investigated by the combination of chemical and cellular-based assays. SeNP average diameters ranged from 171.3 nm up to 216.9 nm; smaller SeNPs were obtained by the utilization of purified pectins, and functionalization with OPE slightly increased the average. At concentrations of 15 mg/L SeNPs were found to be biocompatible, and their toxicity was significantly lower in comparison to inorganic selenium forms. Functionalization of SeNPs with OPE increased their antioxidant activity in chemical models. The effect was not clear in cell-based models, even though all investigated SeNPs improved cell viability and protected intracellular reduced GSH under induced oxidative stress conditions in both investigated cell lines. Exposure of cell lines to SeNPs did not prevent ROS formation after exposure to prooxidant, probably due to low transepithelial permeability. Future studies should focus on further improving the bioavailability/permeability of SeNPs and enhancing the utilization of easily available secondary raw materials in the process of phyto-mediated SeNP synthesis. MDPI 2023-03-06 /pmc/articles/PMC10000393/ /pubmed/36900634 http://dx.doi.org/10.3390/foods12051117 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Golub, Nikolina
Galić, Emerik
Radić, Kristina
Jagodić, Ana-Maria
Predović, Nela
Katelan, Kristina
Tesla, Lucija
Pedisić, Sandra
Vinković, Tomislav
Vitali Čepo, Dubravka
Phyto-Assisted Synthesis of Nanoselenium–Surface Modification and Stabilization by Polyphenols and Pectins Derived from Agricultural Wastes
title Phyto-Assisted Synthesis of Nanoselenium–Surface Modification and Stabilization by Polyphenols and Pectins Derived from Agricultural Wastes
title_full Phyto-Assisted Synthesis of Nanoselenium–Surface Modification and Stabilization by Polyphenols and Pectins Derived from Agricultural Wastes
title_fullStr Phyto-Assisted Synthesis of Nanoselenium–Surface Modification and Stabilization by Polyphenols and Pectins Derived from Agricultural Wastes
title_full_unstemmed Phyto-Assisted Synthesis of Nanoselenium–Surface Modification and Stabilization by Polyphenols and Pectins Derived from Agricultural Wastes
title_short Phyto-Assisted Synthesis of Nanoselenium–Surface Modification and Stabilization by Polyphenols and Pectins Derived from Agricultural Wastes
title_sort phyto-assisted synthesis of nanoselenium–surface modification and stabilization by polyphenols and pectins derived from agricultural wastes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000393/
https://www.ncbi.nlm.nih.gov/pubmed/36900634
http://dx.doi.org/10.3390/foods12051117
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