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Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples

This study aimed to evaluate the performance characteristics of a rapid antigen test developed to detect SARS-CoV-2 (COVID-19), influenza A virus (IAV), and influenza B virus (IBV) (flu) compared with those of the real-time reverse transcription-polymerase chain reaction (rRT-PCR) method. One hundre...

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Autores principales: Dinç, Harika Öykü, Karabulut, Nuran, Alaçam, Sema, Uysal, Hayriye Kırkoyun, Daşdemir, Ferhat Osman, Önel, Mustafa, Tuyji Tok, Yeşim, Sirekbasan, Serhat, Agacfidan, Ali, Gareayaghi, Nesrin, Çakan, Hüseyin, Eryiğit, Önder Yüksel, Kocazeybek, Bekir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000510/
https://www.ncbi.nlm.nih.gov/pubmed/36900116
http://dx.doi.org/10.3390/diagnostics13050972
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author Dinç, Harika Öykü
Karabulut, Nuran
Alaçam, Sema
Uysal, Hayriye Kırkoyun
Daşdemir, Ferhat Osman
Önel, Mustafa
Tuyji Tok, Yeşim
Sirekbasan, Serhat
Agacfidan, Ali
Gareayaghi, Nesrin
Çakan, Hüseyin
Eryiğit, Önder Yüksel
Kocazeybek, Bekir
author_facet Dinç, Harika Öykü
Karabulut, Nuran
Alaçam, Sema
Uysal, Hayriye Kırkoyun
Daşdemir, Ferhat Osman
Önel, Mustafa
Tuyji Tok, Yeşim
Sirekbasan, Serhat
Agacfidan, Ali
Gareayaghi, Nesrin
Çakan, Hüseyin
Eryiğit, Önder Yüksel
Kocazeybek, Bekir
author_sort Dinç, Harika Öykü
collection PubMed
description This study aimed to evaluate the performance characteristics of a rapid antigen test developed to detect SARS-CoV-2 (COVID-19), influenza A virus (IAV), and influenza B virus (IBV) (flu) compared with those of the real-time reverse transcription-polymerase chain reaction (rRT-PCR) method. One hundred SARS-CoV-2, one hundred IAV, and twenty-four IBV patients whose diagnoses were confirmed by clinical and laboratory methods were included in the patient group. Seventy-six patients, who were negative for all respiratory tract viruses, were included as the control group. The Panbio™ COVID-19/Flu A&B Rapid Panel test kit was used in the assays. The sensitivity values of the kit were 97.5%, 97.9%, and 33.33% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load below 20 Ct values. The sensitivity values of the kit were 16.7%, 36.5%, and 11.11% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load above 20 Ct. The kit’s specificity was 100%. In conclusion, this kit demonstrated high sensitivity to SARS-CoV-2 and IAV for viral loads below 20 Ct values, but the sensitivity values were not compatible with PCR positivity for lower viral loads over 20 Ct values. Rapid antigen tests may be preferred as a routine screening tool in communal environments, especially in symptomatic individuals, when diagnosing SARS-CoV-2, IAV, and IBV with high caution.
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spelling pubmed-100005102023-03-11 Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples Dinç, Harika Öykü Karabulut, Nuran Alaçam, Sema Uysal, Hayriye Kırkoyun Daşdemir, Ferhat Osman Önel, Mustafa Tuyji Tok, Yeşim Sirekbasan, Serhat Agacfidan, Ali Gareayaghi, Nesrin Çakan, Hüseyin Eryiğit, Önder Yüksel Kocazeybek, Bekir Diagnostics (Basel) Article This study aimed to evaluate the performance characteristics of a rapid antigen test developed to detect SARS-CoV-2 (COVID-19), influenza A virus (IAV), and influenza B virus (IBV) (flu) compared with those of the real-time reverse transcription-polymerase chain reaction (rRT-PCR) method. One hundred SARS-CoV-2, one hundred IAV, and twenty-four IBV patients whose diagnoses were confirmed by clinical and laboratory methods were included in the patient group. Seventy-six patients, who were negative for all respiratory tract viruses, were included as the control group. The Panbio™ COVID-19/Flu A&B Rapid Panel test kit was used in the assays. The sensitivity values of the kit were 97.5%, 97.9%, and 33.33% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load below 20 Ct values. The sensitivity values of the kit were 16.7%, 36.5%, and 11.11% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load above 20 Ct. The kit’s specificity was 100%. In conclusion, this kit demonstrated high sensitivity to SARS-CoV-2 and IAV for viral loads below 20 Ct values, but the sensitivity values were not compatible with PCR positivity for lower viral loads over 20 Ct values. Rapid antigen tests may be preferred as a routine screening tool in communal environments, especially in symptomatic individuals, when diagnosing SARS-CoV-2, IAV, and IBV with high caution. MDPI 2023-03-03 /pmc/articles/PMC10000510/ /pubmed/36900116 http://dx.doi.org/10.3390/diagnostics13050972 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dinç, Harika Öykü
Karabulut, Nuran
Alaçam, Sema
Uysal, Hayriye Kırkoyun
Daşdemir, Ferhat Osman
Önel, Mustafa
Tuyji Tok, Yeşim
Sirekbasan, Serhat
Agacfidan, Ali
Gareayaghi, Nesrin
Çakan, Hüseyin
Eryiğit, Önder Yüksel
Kocazeybek, Bekir
Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples
title Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples
title_full Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples
title_fullStr Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples
title_full_unstemmed Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples
title_short Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples
title_sort evaluation of the diagnostic performance of a sars-cov-2 and influenza a/b combo rapid antigen test in respiratory samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000510/
https://www.ncbi.nlm.nih.gov/pubmed/36900116
http://dx.doi.org/10.3390/diagnostics13050972
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