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Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples
This study aimed to evaluate the performance characteristics of a rapid antigen test developed to detect SARS-CoV-2 (COVID-19), influenza A virus (IAV), and influenza B virus (IBV) (flu) compared with those of the real-time reverse transcription-polymerase chain reaction (rRT-PCR) method. One hundre...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000510/ https://www.ncbi.nlm.nih.gov/pubmed/36900116 http://dx.doi.org/10.3390/diagnostics13050972 |
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author | Dinç, Harika Öykü Karabulut, Nuran Alaçam, Sema Uysal, Hayriye Kırkoyun Daşdemir, Ferhat Osman Önel, Mustafa Tuyji Tok, Yeşim Sirekbasan, Serhat Agacfidan, Ali Gareayaghi, Nesrin Çakan, Hüseyin Eryiğit, Önder Yüksel Kocazeybek, Bekir |
author_facet | Dinç, Harika Öykü Karabulut, Nuran Alaçam, Sema Uysal, Hayriye Kırkoyun Daşdemir, Ferhat Osman Önel, Mustafa Tuyji Tok, Yeşim Sirekbasan, Serhat Agacfidan, Ali Gareayaghi, Nesrin Çakan, Hüseyin Eryiğit, Önder Yüksel Kocazeybek, Bekir |
author_sort | Dinç, Harika Öykü |
collection | PubMed |
description | This study aimed to evaluate the performance characteristics of a rapid antigen test developed to detect SARS-CoV-2 (COVID-19), influenza A virus (IAV), and influenza B virus (IBV) (flu) compared with those of the real-time reverse transcription-polymerase chain reaction (rRT-PCR) method. One hundred SARS-CoV-2, one hundred IAV, and twenty-four IBV patients whose diagnoses were confirmed by clinical and laboratory methods were included in the patient group. Seventy-six patients, who were negative for all respiratory tract viruses, were included as the control group. The Panbio™ COVID-19/Flu A&B Rapid Panel test kit was used in the assays. The sensitivity values of the kit were 97.5%, 97.9%, and 33.33% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load below 20 Ct values. The sensitivity values of the kit were 16.7%, 36.5%, and 11.11% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load above 20 Ct. The kit’s specificity was 100%. In conclusion, this kit demonstrated high sensitivity to SARS-CoV-2 and IAV for viral loads below 20 Ct values, but the sensitivity values were not compatible with PCR positivity for lower viral loads over 20 Ct values. Rapid antigen tests may be preferred as a routine screening tool in communal environments, especially in symptomatic individuals, when diagnosing SARS-CoV-2, IAV, and IBV with high caution. |
format | Online Article Text |
id | pubmed-10000510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100005102023-03-11 Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples Dinç, Harika Öykü Karabulut, Nuran Alaçam, Sema Uysal, Hayriye Kırkoyun Daşdemir, Ferhat Osman Önel, Mustafa Tuyji Tok, Yeşim Sirekbasan, Serhat Agacfidan, Ali Gareayaghi, Nesrin Çakan, Hüseyin Eryiğit, Önder Yüksel Kocazeybek, Bekir Diagnostics (Basel) Article This study aimed to evaluate the performance characteristics of a rapid antigen test developed to detect SARS-CoV-2 (COVID-19), influenza A virus (IAV), and influenza B virus (IBV) (flu) compared with those of the real-time reverse transcription-polymerase chain reaction (rRT-PCR) method. One hundred SARS-CoV-2, one hundred IAV, and twenty-four IBV patients whose diagnoses were confirmed by clinical and laboratory methods were included in the patient group. Seventy-six patients, who were negative for all respiratory tract viruses, were included as the control group. The Panbio™ COVID-19/Flu A&B Rapid Panel test kit was used in the assays. The sensitivity values of the kit were 97.5%, 97.9%, and 33.33% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load below 20 Ct values. The sensitivity values of the kit were 16.7%, 36.5%, and 11.11% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load above 20 Ct. The kit’s specificity was 100%. In conclusion, this kit demonstrated high sensitivity to SARS-CoV-2 and IAV for viral loads below 20 Ct values, but the sensitivity values were not compatible with PCR positivity for lower viral loads over 20 Ct values. Rapid antigen tests may be preferred as a routine screening tool in communal environments, especially in symptomatic individuals, when diagnosing SARS-CoV-2, IAV, and IBV with high caution. MDPI 2023-03-03 /pmc/articles/PMC10000510/ /pubmed/36900116 http://dx.doi.org/10.3390/diagnostics13050972 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dinç, Harika Öykü Karabulut, Nuran Alaçam, Sema Uysal, Hayriye Kırkoyun Daşdemir, Ferhat Osman Önel, Mustafa Tuyji Tok, Yeşim Sirekbasan, Serhat Agacfidan, Ali Gareayaghi, Nesrin Çakan, Hüseyin Eryiğit, Önder Yüksel Kocazeybek, Bekir Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples |
title | Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples |
title_full | Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples |
title_fullStr | Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples |
title_full_unstemmed | Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples |
title_short | Evaluation of the Diagnostic Performance of a SARS-CoV-2 and Influenza A/B Combo Rapid Antigen Test in Respiratory Samples |
title_sort | evaluation of the diagnostic performance of a sars-cov-2 and influenza a/b combo rapid antigen test in respiratory samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000510/ https://www.ncbi.nlm.nih.gov/pubmed/36900116 http://dx.doi.org/10.3390/diagnostics13050972 |
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