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Sample-minimizing co-flow cell for time-resolved pump–probe X-ray solution scattering

A fundamental problem in biological sciences is understanding how macromolecular machines work and how the structural changes of a molecule are connected to its function. Time-resolved techniques are vital in this regard and essential for understanding the structural dynamics of biomolecules. Time-r...

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Autores principales: Kosheleva, Irina, Henning, Robert, Kim, Insik, Kim, Seong Ok, Kusel, Michael, Srajer, Vukica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000795/
https://www.ncbi.nlm.nih.gov/pubmed/36891863
http://dx.doi.org/10.1107/S1600577522012127
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author Kosheleva, Irina
Henning, Robert
Kim, Insik
Kim, Seong Ok
Kusel, Michael
Srajer, Vukica
author_facet Kosheleva, Irina
Henning, Robert
Kim, Insik
Kim, Seong Ok
Kusel, Michael
Srajer, Vukica
author_sort Kosheleva, Irina
collection PubMed
description A fundamental problem in biological sciences is understanding how macromolecular machines work and how the structural changes of a molecule are connected to its function. Time-resolved techniques are vital in this regard and essential for understanding the structural dynamics of biomolecules. Time-resolved small- and wide-angle X-ray solution scattering has the capability to provide a multitude of information about the kinetics and global structural changes of molecules under their physiological conditions. However, standard protocols for such time-resolved measurements often require significant amounts of sample, which frequently render time-resolved measurements impossible. A cytometry-type sheath co-flow cell, developed at the BioCARS 14-ID beamline at the Advanced Photon Source, USA, allows time-resolved pump–probe X-ray solution scattering measurements to be conducted with sample consumption reduced by more than ten times compared with standard sample cells and protocols. The comparative capabilities of the standard and co-flow experimental setups were demonstrated by studying time-resolved signals in photoactive yellow protein.
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spelling pubmed-100007952023-03-11 Sample-minimizing co-flow cell for time-resolved pump–probe X-ray solution scattering Kosheleva, Irina Henning, Robert Kim, Insik Kim, Seong Ok Kusel, Michael Srajer, Vukica J Synchrotron Radiat Beamlines A fundamental problem in biological sciences is understanding how macromolecular machines work and how the structural changes of a molecule are connected to its function. Time-resolved techniques are vital in this regard and essential for understanding the structural dynamics of biomolecules. Time-resolved small- and wide-angle X-ray solution scattering has the capability to provide a multitude of information about the kinetics and global structural changes of molecules under their physiological conditions. However, standard protocols for such time-resolved measurements often require significant amounts of sample, which frequently render time-resolved measurements impossible. A cytometry-type sheath co-flow cell, developed at the BioCARS 14-ID beamline at the Advanced Photon Source, USA, allows time-resolved pump–probe X-ray solution scattering measurements to be conducted with sample consumption reduced by more than ten times compared with standard sample cells and protocols. The comparative capabilities of the standard and co-flow experimental setups were demonstrated by studying time-resolved signals in photoactive yellow protein. International Union of Crystallography 2023-02-01 /pmc/articles/PMC10000795/ /pubmed/36891863 http://dx.doi.org/10.1107/S1600577522012127 Text en © Irina Kosheleva et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Beamlines
Kosheleva, Irina
Henning, Robert
Kim, Insik
Kim, Seong Ok
Kusel, Michael
Srajer, Vukica
Sample-minimizing co-flow cell for time-resolved pump–probe X-ray solution scattering
title Sample-minimizing co-flow cell for time-resolved pump–probe X-ray solution scattering
title_full Sample-minimizing co-flow cell for time-resolved pump–probe X-ray solution scattering
title_fullStr Sample-minimizing co-flow cell for time-resolved pump–probe X-ray solution scattering
title_full_unstemmed Sample-minimizing co-flow cell for time-resolved pump–probe X-ray solution scattering
title_short Sample-minimizing co-flow cell for time-resolved pump–probe X-ray solution scattering
title_sort sample-minimizing co-flow cell for time-resolved pump–probe x-ray solution scattering
topic Beamlines
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000795/
https://www.ncbi.nlm.nih.gov/pubmed/36891863
http://dx.doi.org/10.1107/S1600577522012127
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