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Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals

X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein c...

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Autores principales: Ang, Artoni Kevin R., Umena, Yasufumi, Sato-Tomita, Ayana, Shibayama, Naoya, Happo, Naohisa, Marumi, Riho, Yamamoto, Yuta, Kimura, Koji, Kawamura, Naomi, Takano, Yu, Matsushita, Tomohiro, Sasaki, Yuji C., Shen, Jian-Ren, Hayashi, Kouichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000799/
https://www.ncbi.nlm.nih.gov/pubmed/36891850
http://dx.doi.org/10.1107/S1600577522011833
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author Ang, Artoni Kevin R.
Umena, Yasufumi
Sato-Tomita, Ayana
Shibayama, Naoya
Happo, Naohisa
Marumi, Riho
Yamamoto, Yuta
Kimura, Koji
Kawamura, Naomi
Takano, Yu
Matsushita, Tomohiro
Sasaki, Yuji C.
Shen, Jian-Ren
Hayashi, Kouichi
author_facet Ang, Artoni Kevin R.
Umena, Yasufumi
Sato-Tomita, Ayana
Shibayama, Naoya
Happo, Naohisa
Marumi, Riho
Yamamoto, Yuta
Kimura, Koji
Kawamura, Naomi
Takano, Yu
Matsushita, Tomohiro
Sasaki, Yuji C.
Shen, Jian-Ren
Hayashi, Kouichi
author_sort Ang, Artoni Kevin R.
collection PubMed
description X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein crystals, the experiment has proven difficult to perform, especially on radiation-sensitive proteins. Here, the development of serial X-ray fluorescence holography to allow the direct recording of hologram patterns before the onset of radiation damage is reported. By combining a 2D hybrid detector and the serial data collection used in serial protein crystallography, the X-ray fluorescence hologram can be directly recorded in a fraction of the measurement time needed for conventional XFH measurements. This approach was demonstrated by obtaining the Mn Kα hologram pattern from the protein crystal Photosystem II without any X-ray-induced reduction of the Mn clusters. Furthermore, a method to interpret the fluorescence patterns as real-space projections of the atoms surrounding the Mn emitters has been developed, where the surrounding atoms produce large dark dips along the emitter–scatterer bond directions. This new technique paves the way for future experiments on protein crystals that aim to clarify the local atomic structures of their functional metal clusters, and for other related XFH experiments such as valence-selective XFH or time-resolved XFH.
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spelling pubmed-100007992023-03-11 Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals Ang, Artoni Kevin R. Umena, Yasufumi Sato-Tomita, Ayana Shibayama, Naoya Happo, Naohisa Marumi, Riho Yamamoto, Yuta Kimura, Koji Kawamura, Naomi Takano, Yu Matsushita, Tomohiro Sasaki, Yuji C. Shen, Jian-Ren Hayashi, Kouichi J Synchrotron Radiat Research Papers X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein crystals, the experiment has proven difficult to perform, especially on radiation-sensitive proteins. Here, the development of serial X-ray fluorescence holography to allow the direct recording of hologram patterns before the onset of radiation damage is reported. By combining a 2D hybrid detector and the serial data collection used in serial protein crystallography, the X-ray fluorescence hologram can be directly recorded in a fraction of the measurement time needed for conventional XFH measurements. This approach was demonstrated by obtaining the Mn Kα hologram pattern from the protein crystal Photosystem II without any X-ray-induced reduction of the Mn clusters. Furthermore, a method to interpret the fluorescence patterns as real-space projections of the atoms surrounding the Mn emitters has been developed, where the surrounding atoms produce large dark dips along the emitter–scatterer bond directions. This new technique paves the way for future experiments on protein crystals that aim to clarify the local atomic structures of their functional metal clusters, and for other related XFH experiments such as valence-selective XFH or time-resolved XFH. International Union of Crystallography 2023-01-20 /pmc/articles/PMC10000799/ /pubmed/36891850 http://dx.doi.org/10.1107/S1600577522011833 Text en © Artoni Kevin R. Ang et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Ang, Artoni Kevin R.
Umena, Yasufumi
Sato-Tomita, Ayana
Shibayama, Naoya
Happo, Naohisa
Marumi, Riho
Yamamoto, Yuta
Kimura, Koji
Kawamura, Naomi
Takano, Yu
Matsushita, Tomohiro
Sasaki, Yuji C.
Shen, Jian-Ren
Hayashi, Kouichi
Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals
title Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals
title_full Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals
title_fullStr Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals
title_full_unstemmed Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals
title_short Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals
title_sort development of serial x-ray fluorescence holography for radiation-sensitive protein crystals
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000799/
https://www.ncbi.nlm.nih.gov/pubmed/36891850
http://dx.doi.org/10.1107/S1600577522011833
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