Cargando…
Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals
X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein c...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000799/ https://www.ncbi.nlm.nih.gov/pubmed/36891850 http://dx.doi.org/10.1107/S1600577522011833 |
_version_ | 1784903970716647424 |
---|---|
author | Ang, Artoni Kevin R. Umena, Yasufumi Sato-Tomita, Ayana Shibayama, Naoya Happo, Naohisa Marumi, Riho Yamamoto, Yuta Kimura, Koji Kawamura, Naomi Takano, Yu Matsushita, Tomohiro Sasaki, Yuji C. Shen, Jian-Ren Hayashi, Kouichi |
author_facet | Ang, Artoni Kevin R. Umena, Yasufumi Sato-Tomita, Ayana Shibayama, Naoya Happo, Naohisa Marumi, Riho Yamamoto, Yuta Kimura, Koji Kawamura, Naomi Takano, Yu Matsushita, Tomohiro Sasaki, Yuji C. Shen, Jian-Ren Hayashi, Kouichi |
author_sort | Ang, Artoni Kevin R. |
collection | PubMed |
description | X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein crystals, the experiment has proven difficult to perform, especially on radiation-sensitive proteins. Here, the development of serial X-ray fluorescence holography to allow the direct recording of hologram patterns before the onset of radiation damage is reported. By combining a 2D hybrid detector and the serial data collection used in serial protein crystallography, the X-ray fluorescence hologram can be directly recorded in a fraction of the measurement time needed for conventional XFH measurements. This approach was demonstrated by obtaining the Mn Kα hologram pattern from the protein crystal Photosystem II without any X-ray-induced reduction of the Mn clusters. Furthermore, a method to interpret the fluorescence patterns as real-space projections of the atoms surrounding the Mn emitters has been developed, where the surrounding atoms produce large dark dips along the emitter–scatterer bond directions. This new technique paves the way for future experiments on protein crystals that aim to clarify the local atomic structures of their functional metal clusters, and for other related XFH experiments such as valence-selective XFH or time-resolved XFH. |
format | Online Article Text |
id | pubmed-10000799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-100007992023-03-11 Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals Ang, Artoni Kevin R. Umena, Yasufumi Sato-Tomita, Ayana Shibayama, Naoya Happo, Naohisa Marumi, Riho Yamamoto, Yuta Kimura, Koji Kawamura, Naomi Takano, Yu Matsushita, Tomohiro Sasaki, Yuji C. Shen, Jian-Ren Hayashi, Kouichi J Synchrotron Radiat Research Papers X-ray fluorescence holography (XFH) is a powerful atomic resolution technique capable of directly imaging the local atomic structure around atoms of a target element within a material. Although it is theoretically possible to use XFH to study the local structures of metal clusters in large protein crystals, the experiment has proven difficult to perform, especially on radiation-sensitive proteins. Here, the development of serial X-ray fluorescence holography to allow the direct recording of hologram patterns before the onset of radiation damage is reported. By combining a 2D hybrid detector and the serial data collection used in serial protein crystallography, the X-ray fluorescence hologram can be directly recorded in a fraction of the measurement time needed for conventional XFH measurements. This approach was demonstrated by obtaining the Mn Kα hologram pattern from the protein crystal Photosystem II without any X-ray-induced reduction of the Mn clusters. Furthermore, a method to interpret the fluorescence patterns as real-space projections of the atoms surrounding the Mn emitters has been developed, where the surrounding atoms produce large dark dips along the emitter–scatterer bond directions. This new technique paves the way for future experiments on protein crystals that aim to clarify the local atomic structures of their functional metal clusters, and for other related XFH experiments such as valence-selective XFH or time-resolved XFH. International Union of Crystallography 2023-01-20 /pmc/articles/PMC10000799/ /pubmed/36891850 http://dx.doi.org/10.1107/S1600577522011833 Text en © Artoni Kevin R. Ang et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Ang, Artoni Kevin R. Umena, Yasufumi Sato-Tomita, Ayana Shibayama, Naoya Happo, Naohisa Marumi, Riho Yamamoto, Yuta Kimura, Koji Kawamura, Naomi Takano, Yu Matsushita, Tomohiro Sasaki, Yuji C. Shen, Jian-Ren Hayashi, Kouichi Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals |
title | Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals |
title_full | Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals |
title_fullStr | Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals |
title_full_unstemmed | Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals |
title_short | Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals |
title_sort | development of serial x-ray fluorescence holography for radiation-sensitive protein crystals |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000799/ https://www.ncbi.nlm.nih.gov/pubmed/36891850 http://dx.doi.org/10.1107/S1600577522011833 |
work_keys_str_mv | AT angartonikevinr developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT umenayasufumi developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT satotomitaayana developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT shibayamanaoya developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT happonaohisa developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT marumiriho developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT yamamotoyuta developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT kimurakoji developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT kawamuranaomi developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT takanoyu developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT matsushitatomohiro developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT sasakiyujic developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT shenjianren developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals AT hayashikouichi developmentofserialxrayfluorescenceholographyforradiationsensitiveproteincrystals |