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Role of SIRT3 in Microgravity Response: A New Player in Muscle Tissue Recovery

Life on Earth has evolved in the presence of a gravity constraint. Any change in the value of such a constraint has important physiological effects. Gravity reduction (microgravity) alters the performance of muscle, bone and, immune systems among others. Therefore, countermeasures to limit such dele...

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Autores principales: Aventaggiato, Michele, Barreca, Federica, Vitiello, Laura, Vespa, Simone, Valente, Sergio, Rotili, Dante, Mai, Antonello, Lotti, Lavinia Vittoria, Sansone, Luigi, Russo, Matteo A., Bizzarri, Mariano, Ferretti, Elisabetta, Tafani, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000945/
https://www.ncbi.nlm.nih.gov/pubmed/36899828
http://dx.doi.org/10.3390/cells12050691
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author Aventaggiato, Michele
Barreca, Federica
Vitiello, Laura
Vespa, Simone
Valente, Sergio
Rotili, Dante
Mai, Antonello
Lotti, Lavinia Vittoria
Sansone, Luigi
Russo, Matteo A.
Bizzarri, Mariano
Ferretti, Elisabetta
Tafani, Marco
author_facet Aventaggiato, Michele
Barreca, Federica
Vitiello, Laura
Vespa, Simone
Valente, Sergio
Rotili, Dante
Mai, Antonello
Lotti, Lavinia Vittoria
Sansone, Luigi
Russo, Matteo A.
Bizzarri, Mariano
Ferretti, Elisabetta
Tafani, Marco
author_sort Aventaggiato, Michele
collection PubMed
description Life on Earth has evolved in the presence of a gravity constraint. Any change in the value of such a constraint has important physiological effects. Gravity reduction (microgravity) alters the performance of muscle, bone and, immune systems among others. Therefore, countermeasures to limit such deleterious effects of microgravity are needed considering future Lunar and Martian missions. Our study aims to demonstrate that the activation of mitochondrial Sirtuin 3 (SIRT3) can be exploited to reduce muscle damage and to maintain muscle differentiation following microgravity exposure. To this effect, we used a RCCS machine to simulate microgravity on ground on a muscle and cardiac cell line. During microgravity, cells were treated with a newly synthesized SIRT3 activator, called MC2791 and vitality, differentiation, ROS and, autophagy/mitophagy were measured. Our results indicate that SIRT3 activation reduces microgravity-induced cell death while maintaining the expression of muscle cell differentiation markers. In conclusion, our study demonstrates that SIRT3 activation could represent a targeted molecular strategy to reduce muscle tissue damage caused by microgravity.
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spelling pubmed-100009452023-03-11 Role of SIRT3 in Microgravity Response: A New Player in Muscle Tissue Recovery Aventaggiato, Michele Barreca, Federica Vitiello, Laura Vespa, Simone Valente, Sergio Rotili, Dante Mai, Antonello Lotti, Lavinia Vittoria Sansone, Luigi Russo, Matteo A. Bizzarri, Mariano Ferretti, Elisabetta Tafani, Marco Cells Article Life on Earth has evolved in the presence of a gravity constraint. Any change in the value of such a constraint has important physiological effects. Gravity reduction (microgravity) alters the performance of muscle, bone and, immune systems among others. Therefore, countermeasures to limit such deleterious effects of microgravity are needed considering future Lunar and Martian missions. Our study aims to demonstrate that the activation of mitochondrial Sirtuin 3 (SIRT3) can be exploited to reduce muscle damage and to maintain muscle differentiation following microgravity exposure. To this effect, we used a RCCS machine to simulate microgravity on ground on a muscle and cardiac cell line. During microgravity, cells were treated with a newly synthesized SIRT3 activator, called MC2791 and vitality, differentiation, ROS and, autophagy/mitophagy were measured. Our results indicate that SIRT3 activation reduces microgravity-induced cell death while maintaining the expression of muscle cell differentiation markers. In conclusion, our study demonstrates that SIRT3 activation could represent a targeted molecular strategy to reduce muscle tissue damage caused by microgravity. MDPI 2023-02-22 /pmc/articles/PMC10000945/ /pubmed/36899828 http://dx.doi.org/10.3390/cells12050691 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aventaggiato, Michele
Barreca, Federica
Vitiello, Laura
Vespa, Simone
Valente, Sergio
Rotili, Dante
Mai, Antonello
Lotti, Lavinia Vittoria
Sansone, Luigi
Russo, Matteo A.
Bizzarri, Mariano
Ferretti, Elisabetta
Tafani, Marco
Role of SIRT3 in Microgravity Response: A New Player in Muscle Tissue Recovery
title Role of SIRT3 in Microgravity Response: A New Player in Muscle Tissue Recovery
title_full Role of SIRT3 in Microgravity Response: A New Player in Muscle Tissue Recovery
title_fullStr Role of SIRT3 in Microgravity Response: A New Player in Muscle Tissue Recovery
title_full_unstemmed Role of SIRT3 in Microgravity Response: A New Player in Muscle Tissue Recovery
title_short Role of SIRT3 in Microgravity Response: A New Player in Muscle Tissue Recovery
title_sort role of sirt3 in microgravity response: a new player in muscle tissue recovery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000945/
https://www.ncbi.nlm.nih.gov/pubmed/36899828
http://dx.doi.org/10.3390/cells12050691
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