Cargando…

Direct Prosthetic Joint Infection Diagnosis from Sonication Fluid Inoculated in Blood Culture Bottles by Direct MALDI-TOF Mass Spectrometry

An accurate and fast microbiological diagnosis is key for a proper management and results when facing prosthetic joint infection (PJI). The purpose of this study is to assess the role of direct Matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS) for early id...

Descripción completa

Detalles Bibliográficos
Autores principales: Beguiristain, Iñaki, Henriquez, Lucia, Sancho, Ignacio, Martin, Carmen, Hidalgo-Ovejero, Angel, Ezpeleta, Carmen, Portillo, Maria Eugenia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001090/
https://www.ncbi.nlm.nih.gov/pubmed/36900086
http://dx.doi.org/10.3390/diagnostics13050942
_version_ 1784904048213753856
author Beguiristain, Iñaki
Henriquez, Lucia
Sancho, Ignacio
Martin, Carmen
Hidalgo-Ovejero, Angel
Ezpeleta, Carmen
Portillo, Maria Eugenia
author_facet Beguiristain, Iñaki
Henriquez, Lucia
Sancho, Ignacio
Martin, Carmen
Hidalgo-Ovejero, Angel
Ezpeleta, Carmen
Portillo, Maria Eugenia
author_sort Beguiristain, Iñaki
collection PubMed
description An accurate and fast microbiological diagnosis is key for a proper management and results when facing prosthetic joint infection (PJI). The purpose of this study is to assess the role of direct Matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS) for early identification of the pathogens causing PJI from sonication fluid inoculated in blood culture bottles (BCB-SF). This prospective multicentric study included 107 consecutive patients from February 2016 to February 2017. Among them, 71 prosthetic joint revision surgeries were undergone for aseptic and 36 for septic reasons. Prostheses were sonicated and the resulting fluid inoculated into blood culture bottles, regardless the suspicion for infection. We assessed the diagnostic performance of direct MALDI-TOF MS identification of the pathogens in BCB-SF and compared it with periprosthetic tissue and conventional sonication fluid cultures. The sensitivity of direct MALDI-TOF MS of BCB-SF (69%) was higher compared to conventional sonication fluid (69% vs. 64%, p > 0.05) or intraoperative tissue cultures (69% vs. 53%, p = 0.04), especially for patients receiving antimicrobial treatment. This approach also reduced the time for identification but the specificity was compromised (100% vs. 94%) and polymicrobial infections were missed. In conclusion, BCB-SF improves the sensitivity and reduces the time of PJI diagnosis when used in combination with conventional cultures under strict sterility conditions.
format Online
Article
Text
id pubmed-10001090
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100010902023-03-11 Direct Prosthetic Joint Infection Diagnosis from Sonication Fluid Inoculated in Blood Culture Bottles by Direct MALDI-TOF Mass Spectrometry Beguiristain, Iñaki Henriquez, Lucia Sancho, Ignacio Martin, Carmen Hidalgo-Ovejero, Angel Ezpeleta, Carmen Portillo, Maria Eugenia Diagnostics (Basel) Article An accurate and fast microbiological diagnosis is key for a proper management and results when facing prosthetic joint infection (PJI). The purpose of this study is to assess the role of direct Matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS) for early identification of the pathogens causing PJI from sonication fluid inoculated in blood culture bottles (BCB-SF). This prospective multicentric study included 107 consecutive patients from February 2016 to February 2017. Among them, 71 prosthetic joint revision surgeries were undergone for aseptic and 36 for septic reasons. Prostheses were sonicated and the resulting fluid inoculated into blood culture bottles, regardless the suspicion for infection. We assessed the diagnostic performance of direct MALDI-TOF MS identification of the pathogens in BCB-SF and compared it with periprosthetic tissue and conventional sonication fluid cultures. The sensitivity of direct MALDI-TOF MS of BCB-SF (69%) was higher compared to conventional sonication fluid (69% vs. 64%, p > 0.05) or intraoperative tissue cultures (69% vs. 53%, p = 0.04), especially for patients receiving antimicrobial treatment. This approach also reduced the time for identification but the specificity was compromised (100% vs. 94%) and polymicrobial infections were missed. In conclusion, BCB-SF improves the sensitivity and reduces the time of PJI diagnosis when used in combination with conventional cultures under strict sterility conditions. MDPI 2023-03-02 /pmc/articles/PMC10001090/ /pubmed/36900086 http://dx.doi.org/10.3390/diagnostics13050942 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Beguiristain, Iñaki
Henriquez, Lucia
Sancho, Ignacio
Martin, Carmen
Hidalgo-Ovejero, Angel
Ezpeleta, Carmen
Portillo, Maria Eugenia
Direct Prosthetic Joint Infection Diagnosis from Sonication Fluid Inoculated in Blood Culture Bottles by Direct MALDI-TOF Mass Spectrometry
title Direct Prosthetic Joint Infection Diagnosis from Sonication Fluid Inoculated in Blood Culture Bottles by Direct MALDI-TOF Mass Spectrometry
title_full Direct Prosthetic Joint Infection Diagnosis from Sonication Fluid Inoculated in Blood Culture Bottles by Direct MALDI-TOF Mass Spectrometry
title_fullStr Direct Prosthetic Joint Infection Diagnosis from Sonication Fluid Inoculated in Blood Culture Bottles by Direct MALDI-TOF Mass Spectrometry
title_full_unstemmed Direct Prosthetic Joint Infection Diagnosis from Sonication Fluid Inoculated in Blood Culture Bottles by Direct MALDI-TOF Mass Spectrometry
title_short Direct Prosthetic Joint Infection Diagnosis from Sonication Fluid Inoculated in Blood Culture Bottles by Direct MALDI-TOF Mass Spectrometry
title_sort direct prosthetic joint infection diagnosis from sonication fluid inoculated in blood culture bottles by direct maldi-tof mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001090/
https://www.ncbi.nlm.nih.gov/pubmed/36900086
http://dx.doi.org/10.3390/diagnostics13050942
work_keys_str_mv AT beguiristaininaki directprostheticjointinfectiondiagnosisfromsonicationfluidinoculatedinbloodculturebottlesbydirectmalditofmassspectrometry
AT henriquezlucia directprostheticjointinfectiondiagnosisfromsonicationfluidinoculatedinbloodculturebottlesbydirectmalditofmassspectrometry
AT sanchoignacio directprostheticjointinfectiondiagnosisfromsonicationfluidinoculatedinbloodculturebottlesbydirectmalditofmassspectrometry
AT martincarmen directprostheticjointinfectiondiagnosisfromsonicationfluidinoculatedinbloodculturebottlesbydirectmalditofmassspectrometry
AT hidalgoovejeroangel directprostheticjointinfectiondiagnosisfromsonicationfluidinoculatedinbloodculturebottlesbydirectmalditofmassspectrometry
AT ezpeletacarmen directprostheticjointinfectiondiagnosisfromsonicationfluidinoculatedinbloodculturebottlesbydirectmalditofmassspectrometry
AT portillomariaeugenia directprostheticjointinfectiondiagnosisfromsonicationfluidinoculatedinbloodculturebottlesbydirectmalditofmassspectrometry