Cargando…

The Hybrid Capture 2 Results in Correlation with the Pap Test, Sexual Behavior, and Characteristics of Romanian Women

Background: Human papillomavirus (HPV) infection is the major cause of cervical cancer (CC); hence, it is critical to understand the processes by which HPV infection causes squamous intraepithelial lesions, as well as the proper diagnostic tools. The objective of this study was to establish the corr...

Descripción completa

Detalles Bibliográficos
Autores principales: Voidăzan, Toader Septimiu, Uzun, Cosmina Cristina, Kovacs, Zsolt, Rosznayai, Florin Francisc, Turdean, Sabin Gligore, Budianu, Mihaela-Alexandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001103/
https://www.ncbi.nlm.nih.gov/pubmed/36900850
http://dx.doi.org/10.3390/ijerph20053839
_version_ 1784904051531448320
author Voidăzan, Toader Septimiu
Uzun, Cosmina Cristina
Kovacs, Zsolt
Rosznayai, Florin Francisc
Turdean, Sabin Gligore
Budianu, Mihaela-Alexandra
author_facet Voidăzan, Toader Septimiu
Uzun, Cosmina Cristina
Kovacs, Zsolt
Rosznayai, Florin Francisc
Turdean, Sabin Gligore
Budianu, Mihaela-Alexandra
author_sort Voidăzan, Toader Septimiu
collection PubMed
description Background: Human papillomavirus (HPV) infection is the major cause of cervical cancer (CC); hence, it is critical to understand the processes by which HPV infection causes squamous intraepithelial lesions, as well as the proper diagnostic tools. The objective of this study was to establish the correlations between Pap test results and Hybrid Capture 2 (HC2) tests findings. Materials and methods: This study included 169 women between the ages of 30 and 64, who presented for consultation in gynecological clinics in both the public and the private sectors. These women reported symptoms, such as abnormal vaginal discharge and genital irritation, as well as early onset of sexual activity, multiple sexual partners, history of other sexually transmitted infections or high-risk sexual partners, immunosuppression, or tobacco smoking. Pap tests and HPV testing, using the HC2 method, were performed for the women enrolled in the study, including data gathered after patients completed questionnaires concerning their sexual behavior. Results: The HC2 method revealed that 66 patients (39.1%) tested positive for high-risk HPV types. Of the patients with positive results, 14 (21.2%) presented Atypical Squamous Cells of Undetermined Significance (ASC-US) compared to 10 (9.7%) patients in the negative group (p = 0.042). Atypical Squamous Cells for which a high-grade lesion cannot be excluded (ASC-H) were identified primarily in women with positive HC2 (6.1%). HR-HPV positivity was substantially more associated with low-grade ASC-US or low-grade squamous intraepithelial lesion (LSIL) and high-grade ASC-H cytology (OR = 2.53; 95% CI: 1.10–5.80, respectively, OR = 14.9; 95%CI: 1.006–34.59). Unmarried women (31.8%; p = 0.004) and women with multiple partners (over four partners, 10.6%; p = 0.03) were more likely to have an HPV infection when compared to married women and those with fewer sexual partners. Conclusions: Understanding the epidemiology of HPV genital infections is essential for developing preventive measures against this infection and CC. Identifying the most prevalent HPV types, and determining the incidence of HPV oncogenic infections, in conjunction with Pap test results and sexual behavior information, can constitute part of an algorithm for the efficient management of cervical intraepithelial lesions.
format Online
Article
Text
id pubmed-10001103
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100011032023-03-11 The Hybrid Capture 2 Results in Correlation with the Pap Test, Sexual Behavior, and Characteristics of Romanian Women Voidăzan, Toader Septimiu Uzun, Cosmina Cristina Kovacs, Zsolt Rosznayai, Florin Francisc Turdean, Sabin Gligore Budianu, Mihaela-Alexandra Int J Environ Res Public Health Article Background: Human papillomavirus (HPV) infection is the major cause of cervical cancer (CC); hence, it is critical to understand the processes by which HPV infection causes squamous intraepithelial lesions, as well as the proper diagnostic tools. The objective of this study was to establish the correlations between Pap test results and Hybrid Capture 2 (HC2) tests findings. Materials and methods: This study included 169 women between the ages of 30 and 64, who presented for consultation in gynecological clinics in both the public and the private sectors. These women reported symptoms, such as abnormal vaginal discharge and genital irritation, as well as early onset of sexual activity, multiple sexual partners, history of other sexually transmitted infections or high-risk sexual partners, immunosuppression, or tobacco smoking. Pap tests and HPV testing, using the HC2 method, were performed for the women enrolled in the study, including data gathered after patients completed questionnaires concerning their sexual behavior. Results: The HC2 method revealed that 66 patients (39.1%) tested positive for high-risk HPV types. Of the patients with positive results, 14 (21.2%) presented Atypical Squamous Cells of Undetermined Significance (ASC-US) compared to 10 (9.7%) patients in the negative group (p = 0.042). Atypical Squamous Cells for which a high-grade lesion cannot be excluded (ASC-H) were identified primarily in women with positive HC2 (6.1%). HR-HPV positivity was substantially more associated with low-grade ASC-US or low-grade squamous intraepithelial lesion (LSIL) and high-grade ASC-H cytology (OR = 2.53; 95% CI: 1.10–5.80, respectively, OR = 14.9; 95%CI: 1.006–34.59). Unmarried women (31.8%; p = 0.004) and women with multiple partners (over four partners, 10.6%; p = 0.03) were more likely to have an HPV infection when compared to married women and those with fewer sexual partners. Conclusions: Understanding the epidemiology of HPV genital infections is essential for developing preventive measures against this infection and CC. Identifying the most prevalent HPV types, and determining the incidence of HPV oncogenic infections, in conjunction with Pap test results and sexual behavior information, can constitute part of an algorithm for the efficient management of cervical intraepithelial lesions. MDPI 2023-02-21 /pmc/articles/PMC10001103/ /pubmed/36900850 http://dx.doi.org/10.3390/ijerph20053839 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Voidăzan, Toader Septimiu
Uzun, Cosmina Cristina
Kovacs, Zsolt
Rosznayai, Florin Francisc
Turdean, Sabin Gligore
Budianu, Mihaela-Alexandra
The Hybrid Capture 2 Results in Correlation with the Pap Test, Sexual Behavior, and Characteristics of Romanian Women
title The Hybrid Capture 2 Results in Correlation with the Pap Test, Sexual Behavior, and Characteristics of Romanian Women
title_full The Hybrid Capture 2 Results in Correlation with the Pap Test, Sexual Behavior, and Characteristics of Romanian Women
title_fullStr The Hybrid Capture 2 Results in Correlation with the Pap Test, Sexual Behavior, and Characteristics of Romanian Women
title_full_unstemmed The Hybrid Capture 2 Results in Correlation with the Pap Test, Sexual Behavior, and Characteristics of Romanian Women
title_short The Hybrid Capture 2 Results in Correlation with the Pap Test, Sexual Behavior, and Characteristics of Romanian Women
title_sort hybrid capture 2 results in correlation with the pap test, sexual behavior, and characteristics of romanian women
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001103/
https://www.ncbi.nlm.nih.gov/pubmed/36900850
http://dx.doi.org/10.3390/ijerph20053839
work_keys_str_mv AT voidazantoaderseptimiu thehybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT uzuncosminacristina thehybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT kovacszsolt thehybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT rosznayaiflorinfrancisc thehybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT turdeansabingligore thehybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT budianumihaelaalexandra thehybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT voidazantoaderseptimiu hybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT uzuncosminacristina hybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT kovacszsolt hybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT rosznayaiflorinfrancisc hybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT turdeansabingligore hybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen
AT budianumihaelaalexandra hybridcapture2resultsincorrelationwiththepaptestsexualbehaviorandcharacteristicsofromanianwomen