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Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy

Gut microbiota imbalance is associated with the occurrence of metabolic diseases such as obesity. Thus, its modulation is a promising strategy to restore gut microbiota and improve intestinal health in the obese. This paper examines the role of probiotics, antimicrobials, and diet in modulating gut...

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Autores principales: de Moura e Dias, Mariana, da Silva Duarte, Vinícius, Mota, Lúcio Flávio Macedo, de Cássia Ávila Alpino, Gabriela, dos Reis Louzano, Sandra Aparecida, da Conceição, Lisiane Lopes, Mantovanie, Hilário Cuquetto, Pereira, Solange Silveira, Oliveira, Leandro Licursi, de Oliveira Mendes, Tiago Antônio, Porcellato, Davide, do Carmo Gouveia Peluzio, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001121/
https://www.ncbi.nlm.nih.gov/pubmed/36900609
http://dx.doi.org/10.3390/foods12051092
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author de Moura e Dias, Mariana
da Silva Duarte, Vinícius
Mota, Lúcio Flávio Macedo
de Cássia Ávila Alpino, Gabriela
dos Reis Louzano, Sandra Aparecida
da Conceição, Lisiane Lopes
Mantovanie, Hilário Cuquetto
Pereira, Solange Silveira
Oliveira, Leandro Licursi
de Oliveira Mendes, Tiago Antônio
Porcellato, Davide
do Carmo Gouveia Peluzio, Maria
author_facet de Moura e Dias, Mariana
da Silva Duarte, Vinícius
Mota, Lúcio Flávio Macedo
de Cássia Ávila Alpino, Gabriela
dos Reis Louzano, Sandra Aparecida
da Conceição, Lisiane Lopes
Mantovanie, Hilário Cuquetto
Pereira, Solange Silveira
Oliveira, Leandro Licursi
de Oliveira Mendes, Tiago Antônio
Porcellato, Davide
do Carmo Gouveia Peluzio, Maria
author_sort de Moura e Dias, Mariana
collection PubMed
description Gut microbiota imbalance is associated with the occurrence of metabolic diseases such as obesity. Thus, its modulation is a promising strategy to restore gut microbiota and improve intestinal health in the obese. This paper examines the role of probiotics, antimicrobials, and diet in modulating gut microbiota and improving intestinal health. Accordingly, obesity was induced in C57BL/6J mice, after which they were redistributed and fed with an obesogenic diet (intervention A) or standard AIN-93 diet (intervention B). Concomitantly, all the groups underwent a treatment phase with Lactobacillus gasseri LG-G12, ceftriaxone, or ceftriaxone followed by L. gasseri LG-G12. At the end of the experimental period, the following analysis was conducted: metataxonomic analysis, functional profiling of gut microbiota, intestinal permeability, and caecal concentration of short-chain fatty acids. High-fat diet impaired bacterial diversity/richness, which was counteracted in association with L. gasseri LG-G12 and the AIN-93 diet. Additionally, SCFA-producing bacteria were negatively correlated with high intestinal permeability parameters, which was further confirmed via functional profile prediction of the gut microbiota. A novel perspective on anti-obesity probiotics is presented by these findings based on the improvement of intestinal health irrespective of undergoing antimicrobial therapy or not.
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spelling pubmed-100011212023-03-11 Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy de Moura e Dias, Mariana da Silva Duarte, Vinícius Mota, Lúcio Flávio Macedo de Cássia Ávila Alpino, Gabriela dos Reis Louzano, Sandra Aparecida da Conceição, Lisiane Lopes Mantovanie, Hilário Cuquetto Pereira, Solange Silveira Oliveira, Leandro Licursi de Oliveira Mendes, Tiago Antônio Porcellato, Davide do Carmo Gouveia Peluzio, Maria Foods Article Gut microbiota imbalance is associated with the occurrence of metabolic diseases such as obesity. Thus, its modulation is a promising strategy to restore gut microbiota and improve intestinal health in the obese. This paper examines the role of probiotics, antimicrobials, and diet in modulating gut microbiota and improving intestinal health. Accordingly, obesity was induced in C57BL/6J mice, after which they were redistributed and fed with an obesogenic diet (intervention A) or standard AIN-93 diet (intervention B). Concomitantly, all the groups underwent a treatment phase with Lactobacillus gasseri LG-G12, ceftriaxone, or ceftriaxone followed by L. gasseri LG-G12. At the end of the experimental period, the following analysis was conducted: metataxonomic analysis, functional profiling of gut microbiota, intestinal permeability, and caecal concentration of short-chain fatty acids. High-fat diet impaired bacterial diversity/richness, which was counteracted in association with L. gasseri LG-G12 and the AIN-93 diet. Additionally, SCFA-producing bacteria were negatively correlated with high intestinal permeability parameters, which was further confirmed via functional profile prediction of the gut microbiota. A novel perspective on anti-obesity probiotics is presented by these findings based on the improvement of intestinal health irrespective of undergoing antimicrobial therapy or not. MDPI 2023-03-03 /pmc/articles/PMC10001121/ /pubmed/36900609 http://dx.doi.org/10.3390/foods12051092 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Moura e Dias, Mariana
da Silva Duarte, Vinícius
Mota, Lúcio Flávio Macedo
de Cássia Ávila Alpino, Gabriela
dos Reis Louzano, Sandra Aparecida
da Conceição, Lisiane Lopes
Mantovanie, Hilário Cuquetto
Pereira, Solange Silveira
Oliveira, Leandro Licursi
de Oliveira Mendes, Tiago Antônio
Porcellato, Davide
do Carmo Gouveia Peluzio, Maria
Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy
title Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy
title_full Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy
title_fullStr Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy
title_full_unstemmed Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy
title_short Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy
title_sort lactobacillus gasseri lg-g12 restores gut microbiota and intestinal health in obesity mice on ceftriaxone therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001121/
https://www.ncbi.nlm.nih.gov/pubmed/36900609
http://dx.doi.org/10.3390/foods12051092
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