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Vancomycin and Linezolid-Resistant Enterococcus Isolates from a Tertiary Care Center in India

Introduction: There is increasing development of antibiotic resistance among the Enterococcus species. Objectives: This study was performed to determine prevalence and characterize the vancomycin-resistant and linezolid-resistant enterococcus isolates from a tertiary care center. Moreover, the antim...

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Autores principales: Sengupta, Mallika, Sarkar, Riya, Sarkar, Soma, Sengupta, Manideepa, Ghosh, Sougata, Banerjee, Parthajit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001185/
https://www.ncbi.nlm.nih.gov/pubmed/36900089
http://dx.doi.org/10.3390/diagnostics13050945
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author Sengupta, Mallika
Sarkar, Riya
Sarkar, Soma
Sengupta, Manideepa
Ghosh, Sougata
Banerjee, Parthajit
author_facet Sengupta, Mallika
Sarkar, Riya
Sarkar, Soma
Sengupta, Manideepa
Ghosh, Sougata
Banerjee, Parthajit
author_sort Sengupta, Mallika
collection PubMed
description Introduction: There is increasing development of antibiotic resistance among the Enterococcus species. Objectives: This study was performed to determine prevalence and characterize the vancomycin-resistant and linezolid-resistant enterococcus isolates from a tertiary care center. Moreover, the antimicrobial susceptibility pattern of these isolates was also determined. Materials and Methods: A prospective study was performed in Medical College, Kolkata, India, over a period of two years (from January 2018 to December 2019). After obtaining clearance from the Institutional Ethics Committee, Enterococcus isolates from various samples were included in the present investigation. In addition to the various conventional biochemical tests, the VITEK 2 Compact system was used to identify the Enterococcus species. The isolates were tested for antimicrobial susceptibility to different antibiotics using the Kirby–Bauer disk diffusion method and VITEK 2 Compact to determine the minimum inhibitory concentration (MIC). The Clinical and Laboratory Standards Institute (CLSI) 2017 guidelines were used to interpret susceptibility. Multiplex PCR was performed for genetic characterization of the vancomycin-resistant Enterococcus isolates and sequencing was performed for characterization of the linezolid-resistant Enterococcus isolates. Results: During the period of two years, 371 isolates of Enterococcus spp. were obtained from 4934 clinical isolates showing a prevalence of 7.52%. Among these isolates, 239 (64.42%) were Enterococcus faecalis, 114 (30.72%) Enterococcus faecium, and others were Enterococcus durans, Enterococcus casseliflavus, Enterococcus gallinarum, and Enterococcus avium. Among these, 24 (6.47%) were VRE (Vancomycin-Resistant Enterococcus) of which 18 isolates were Van A type and six isolates of Enterococcus casseliflavus and Enterococcus gallinarum were resistant VanC type. There were two linezolid-resistant Enterococcus, and they were found to have the G2576T mutation. Among the 371 isolates, 252 (67.92%) were multi-drug resistant. Conclusion: This study found an increasing prevalence of vancomycin-resistant Enterococcus isolates. There is also an alarming prevalence of multidrug resistance among these isolates.
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spelling pubmed-100011852023-03-11 Vancomycin and Linezolid-Resistant Enterococcus Isolates from a Tertiary Care Center in India Sengupta, Mallika Sarkar, Riya Sarkar, Soma Sengupta, Manideepa Ghosh, Sougata Banerjee, Parthajit Diagnostics (Basel) Article Introduction: There is increasing development of antibiotic resistance among the Enterococcus species. Objectives: This study was performed to determine prevalence and characterize the vancomycin-resistant and linezolid-resistant enterococcus isolates from a tertiary care center. Moreover, the antimicrobial susceptibility pattern of these isolates was also determined. Materials and Methods: A prospective study was performed in Medical College, Kolkata, India, over a period of two years (from January 2018 to December 2019). After obtaining clearance from the Institutional Ethics Committee, Enterococcus isolates from various samples were included in the present investigation. In addition to the various conventional biochemical tests, the VITEK 2 Compact system was used to identify the Enterococcus species. The isolates were tested for antimicrobial susceptibility to different antibiotics using the Kirby–Bauer disk diffusion method and VITEK 2 Compact to determine the minimum inhibitory concentration (MIC). The Clinical and Laboratory Standards Institute (CLSI) 2017 guidelines were used to interpret susceptibility. Multiplex PCR was performed for genetic characterization of the vancomycin-resistant Enterococcus isolates and sequencing was performed for characterization of the linezolid-resistant Enterococcus isolates. Results: During the period of two years, 371 isolates of Enterococcus spp. were obtained from 4934 clinical isolates showing a prevalence of 7.52%. Among these isolates, 239 (64.42%) were Enterococcus faecalis, 114 (30.72%) Enterococcus faecium, and others were Enterococcus durans, Enterococcus casseliflavus, Enterococcus gallinarum, and Enterococcus avium. Among these, 24 (6.47%) were VRE (Vancomycin-Resistant Enterococcus) of which 18 isolates were Van A type and six isolates of Enterococcus casseliflavus and Enterococcus gallinarum were resistant VanC type. There were two linezolid-resistant Enterococcus, and they were found to have the G2576T mutation. Among the 371 isolates, 252 (67.92%) were multi-drug resistant. Conclusion: This study found an increasing prevalence of vancomycin-resistant Enterococcus isolates. There is also an alarming prevalence of multidrug resistance among these isolates. MDPI 2023-03-02 /pmc/articles/PMC10001185/ /pubmed/36900089 http://dx.doi.org/10.3390/diagnostics13050945 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sengupta, Mallika
Sarkar, Riya
Sarkar, Soma
Sengupta, Manideepa
Ghosh, Sougata
Banerjee, Parthajit
Vancomycin and Linezolid-Resistant Enterococcus Isolates from a Tertiary Care Center in India
title Vancomycin and Linezolid-Resistant Enterococcus Isolates from a Tertiary Care Center in India
title_full Vancomycin and Linezolid-Resistant Enterococcus Isolates from a Tertiary Care Center in India
title_fullStr Vancomycin and Linezolid-Resistant Enterococcus Isolates from a Tertiary Care Center in India
title_full_unstemmed Vancomycin and Linezolid-Resistant Enterococcus Isolates from a Tertiary Care Center in India
title_short Vancomycin and Linezolid-Resistant Enterococcus Isolates from a Tertiary Care Center in India
title_sort vancomycin and linezolid-resistant enterococcus isolates from a tertiary care center in india
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001185/
https://www.ncbi.nlm.nih.gov/pubmed/36900089
http://dx.doi.org/10.3390/diagnostics13050945
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