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Development Control and Inactivation of Byssochlamys nivea Ascospores by Hyperbaric Storage at Room Temperature
This study tested hyperbaric storage (25–150 MPa, for 30 days) at room-temperature (HS/RT, 18–23 °C) in order to control the development of Byssochlamys nivea ascospores in apple juice. In order to mimic commercially pasteurized juice contaminated with ascospores, thermal pasteurization (70 and 80 °...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001197/ https://www.ncbi.nlm.nih.gov/pubmed/36900495 http://dx.doi.org/10.3390/foods12050978 |
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author | Pinto, Carlos A. Galante, Diogo Espinoza-Suarez, Edelman Gaspar, Vítor M. Mano, João F. Barba, Francisco J. Saraiva, Jorge A. |
author_facet | Pinto, Carlos A. Galante, Diogo Espinoza-Suarez, Edelman Gaspar, Vítor M. Mano, João F. Barba, Francisco J. Saraiva, Jorge A. |
author_sort | Pinto, Carlos A. |
collection | PubMed |
description | This study tested hyperbaric storage (25–150 MPa, for 30 days) at room-temperature (HS/RT, 18–23 °C) in order to control the development of Byssochlamys nivea ascospores in apple juice. In order to mimic commercially pasteurized juice contaminated with ascospores, thermal pasteurization (70 and 80 °C for 30 s) and nonthermal high pressure pasteurization (600 MPa for 3 min at 17 °C, HPP) took place, and the juice was afterwards placed under HS/RT conditions. Control samples were also placed in atmospheric pressure (AP) conditions at RT and were refrigerated (4 °C). The results showed that HS/RT, in samples without a pasteurization step and those pasteurized at 70 °C/30 s, was able to inhibit ascospore development, contrarily to samples at AP/RT and refrigeration. HS/RT for samples pasteurized at 80 °C/30 s evidenced ascospore inactivation, especially at 150 MPa, wherein an overall reduction of at least 4.73 log units of ascospores was observed to below detection limits (1.00 Log CFU/mL); meanwhile, for HPP samples, especially at 75 and 150 MPa, an overall reduction of 3 log units (to below quantification limits, 2.00 Log CFU/mL) was observed. Phase-contrast microscopy revealed that the ascospores do not complete the germination process under HS/RT, hence avoiding hyphae formation, which is important for food safety since mycotoxin development occurs only after hyphae formation. These findings suggest that HS/RT is a safe food preservation methodology, as it prevents ascospore development and inactivates them following commercial-like thermal or nonthermal HPP pasteurization, preventing mycotoxin production and enhancing ascospore inactivation. |
format | Online Article Text |
id | pubmed-10001197 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100011972023-03-11 Development Control and Inactivation of Byssochlamys nivea Ascospores by Hyperbaric Storage at Room Temperature Pinto, Carlos A. Galante, Diogo Espinoza-Suarez, Edelman Gaspar, Vítor M. Mano, João F. Barba, Francisco J. Saraiva, Jorge A. Foods Article This study tested hyperbaric storage (25–150 MPa, for 30 days) at room-temperature (HS/RT, 18–23 °C) in order to control the development of Byssochlamys nivea ascospores in apple juice. In order to mimic commercially pasteurized juice contaminated with ascospores, thermal pasteurization (70 and 80 °C for 30 s) and nonthermal high pressure pasteurization (600 MPa for 3 min at 17 °C, HPP) took place, and the juice was afterwards placed under HS/RT conditions. Control samples were also placed in atmospheric pressure (AP) conditions at RT and were refrigerated (4 °C). The results showed that HS/RT, in samples without a pasteurization step and those pasteurized at 70 °C/30 s, was able to inhibit ascospore development, contrarily to samples at AP/RT and refrigeration. HS/RT for samples pasteurized at 80 °C/30 s evidenced ascospore inactivation, especially at 150 MPa, wherein an overall reduction of at least 4.73 log units of ascospores was observed to below detection limits (1.00 Log CFU/mL); meanwhile, for HPP samples, especially at 75 and 150 MPa, an overall reduction of 3 log units (to below quantification limits, 2.00 Log CFU/mL) was observed. Phase-contrast microscopy revealed that the ascospores do not complete the germination process under HS/RT, hence avoiding hyphae formation, which is important for food safety since mycotoxin development occurs only after hyphae formation. These findings suggest that HS/RT is a safe food preservation methodology, as it prevents ascospore development and inactivates them following commercial-like thermal or nonthermal HPP pasteurization, preventing mycotoxin production and enhancing ascospore inactivation. MDPI 2023-02-25 /pmc/articles/PMC10001197/ /pubmed/36900495 http://dx.doi.org/10.3390/foods12050978 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pinto, Carlos A. Galante, Diogo Espinoza-Suarez, Edelman Gaspar, Vítor M. Mano, João F. Barba, Francisco J. Saraiva, Jorge A. Development Control and Inactivation of Byssochlamys nivea Ascospores by Hyperbaric Storage at Room Temperature |
title | Development Control and Inactivation of Byssochlamys nivea Ascospores by Hyperbaric Storage at Room Temperature |
title_full | Development Control and Inactivation of Byssochlamys nivea Ascospores by Hyperbaric Storage at Room Temperature |
title_fullStr | Development Control and Inactivation of Byssochlamys nivea Ascospores by Hyperbaric Storage at Room Temperature |
title_full_unstemmed | Development Control and Inactivation of Byssochlamys nivea Ascospores by Hyperbaric Storage at Room Temperature |
title_short | Development Control and Inactivation of Byssochlamys nivea Ascospores by Hyperbaric Storage at Room Temperature |
title_sort | development control and inactivation of byssochlamys nivea ascospores by hyperbaric storage at room temperature |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001197/ https://www.ncbi.nlm.nih.gov/pubmed/36900495 http://dx.doi.org/10.3390/foods12050978 |
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