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Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects

Functionalizing biomaterials with conditioned media (CM) from mesenchymal stromal cells (MSC) is a promising strategy for enhancing the outcomes of guided bone regeneration (GBR). This study aimed to evaluate the bone regenerative potential of collagen membranes (MEM) functionalized with CM from hum...

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Autores principales: Shanbhag, Siddharth, Kampleitner, Carina, Al-Sharabi, Niyaz, Mohamed-Ahmed, Samih, Apaza Alccayhuaman, Karol Ali, Heimel, Patrick, Tangl, Stefan, Beinlich, Andreas, Rana, Neha, Sanz, Mariano, Kristoffersen, Einar K., Mustafa, Kamal, Gruber, Reinhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001262/
https://www.ncbi.nlm.nih.gov/pubmed/36899904
http://dx.doi.org/10.3390/cells12050767
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author Shanbhag, Siddharth
Kampleitner, Carina
Al-Sharabi, Niyaz
Mohamed-Ahmed, Samih
Apaza Alccayhuaman, Karol Ali
Heimel, Patrick
Tangl, Stefan
Beinlich, Andreas
Rana, Neha
Sanz, Mariano
Kristoffersen, Einar K.
Mustafa, Kamal
Gruber, Reinhard
author_facet Shanbhag, Siddharth
Kampleitner, Carina
Al-Sharabi, Niyaz
Mohamed-Ahmed, Samih
Apaza Alccayhuaman, Karol Ali
Heimel, Patrick
Tangl, Stefan
Beinlich, Andreas
Rana, Neha
Sanz, Mariano
Kristoffersen, Einar K.
Mustafa, Kamal
Gruber, Reinhard
author_sort Shanbhag, Siddharth
collection PubMed
description Functionalizing biomaterials with conditioned media (CM) from mesenchymal stromal cells (MSC) is a promising strategy for enhancing the outcomes of guided bone regeneration (GBR). This study aimed to evaluate the bone regenerative potential of collagen membranes (MEM) functionalized with CM from human bone marrow MSC (MEM-CM) in critical size rat calvarial defects. MEM-CM prepared via soaking (CM-SOAK) or soaking followed by lyophilization (CM-LYO) were applied to critical size rat calvarial defects. Control treatments included native MEM, MEM with rat MSC (CEL) and no treatment. New bone formation was analyzed via micro-CT (2 and 4 weeks) and histology (4 weeks). Greater radiographic new bone formation occurred at 2 weeks in the CM-LYO group vs. all other groups. After 4 weeks, only the CM-LYO group was superior to the untreated control group, whereas the CM-SOAK, CEL and native MEM groups were similar. Histologically, the regenerated tissues showed a combination of regular new bone and hybrid new bone, which formed within the membrane compartment and was characterized by the incorporation of mineralized MEM fibers. Areas of new bone formation and MEM mineralization were greatest in the CM-LYO group. Proteomic analysis of lyophilized CM revealed the enrichment of several proteins and biological processes related to bone formation. In summary, lyophilized MEM-CM enhanced new bone formation in rat calvarial defects, thus representing a novel ‘off-the-shelf’ strategy for GBR.
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spelling pubmed-100012622023-03-11 Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects Shanbhag, Siddharth Kampleitner, Carina Al-Sharabi, Niyaz Mohamed-Ahmed, Samih Apaza Alccayhuaman, Karol Ali Heimel, Patrick Tangl, Stefan Beinlich, Andreas Rana, Neha Sanz, Mariano Kristoffersen, Einar K. Mustafa, Kamal Gruber, Reinhard Cells Article Functionalizing biomaterials with conditioned media (CM) from mesenchymal stromal cells (MSC) is a promising strategy for enhancing the outcomes of guided bone regeneration (GBR). This study aimed to evaluate the bone regenerative potential of collagen membranes (MEM) functionalized with CM from human bone marrow MSC (MEM-CM) in critical size rat calvarial defects. MEM-CM prepared via soaking (CM-SOAK) or soaking followed by lyophilization (CM-LYO) were applied to critical size rat calvarial defects. Control treatments included native MEM, MEM with rat MSC (CEL) and no treatment. New bone formation was analyzed via micro-CT (2 and 4 weeks) and histology (4 weeks). Greater radiographic new bone formation occurred at 2 weeks in the CM-LYO group vs. all other groups. After 4 weeks, only the CM-LYO group was superior to the untreated control group, whereas the CM-SOAK, CEL and native MEM groups were similar. Histologically, the regenerated tissues showed a combination of regular new bone and hybrid new bone, which formed within the membrane compartment and was characterized by the incorporation of mineralized MEM fibers. Areas of new bone formation and MEM mineralization were greatest in the CM-LYO group. Proteomic analysis of lyophilized CM revealed the enrichment of several proteins and biological processes related to bone formation. In summary, lyophilized MEM-CM enhanced new bone formation in rat calvarial defects, thus representing a novel ‘off-the-shelf’ strategy for GBR. MDPI 2023-02-28 /pmc/articles/PMC10001262/ /pubmed/36899904 http://dx.doi.org/10.3390/cells12050767 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shanbhag, Siddharth
Kampleitner, Carina
Al-Sharabi, Niyaz
Mohamed-Ahmed, Samih
Apaza Alccayhuaman, Karol Ali
Heimel, Patrick
Tangl, Stefan
Beinlich, Andreas
Rana, Neha
Sanz, Mariano
Kristoffersen, Einar K.
Mustafa, Kamal
Gruber, Reinhard
Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects
title Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects
title_full Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects
title_fullStr Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects
title_full_unstemmed Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects
title_short Functionalizing Collagen Membranes with MSC-Conditioned Media Promotes Guided Bone Regeneration in Rat Calvarial Defects
title_sort functionalizing collagen membranes with msc-conditioned media promotes guided bone regeneration in rat calvarial defects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001262/
https://www.ncbi.nlm.nih.gov/pubmed/36899904
http://dx.doi.org/10.3390/cells12050767
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