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Inherently Antimicrobial P(MMA-ran-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing

In this work, two compounds belonging to the BODIPY family, and previously investigated for their photosensitizing properties, have been bound to the amino-pendant groups of three random copolymers, with different amounts of methyl methacrylate (MMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA)...

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Autores principales: Santoro, Orlando, Malacarne, Miryam Chiara, Sarcone, Francesco, Scapinello, Luca, Pragliola, Stefania, Caruso, Enrico, Orlandi, Viviana Teresa, Izzo, Lorella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001570/
https://www.ncbi.nlm.nih.gov/pubmed/36901769
http://dx.doi.org/10.3390/ijms24054340
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author Santoro, Orlando
Malacarne, Miryam Chiara
Sarcone, Francesco
Scapinello, Luca
Pragliola, Stefania
Caruso, Enrico
Orlandi, Viviana Teresa
Izzo, Lorella
author_facet Santoro, Orlando
Malacarne, Miryam Chiara
Sarcone, Francesco
Scapinello, Luca
Pragliola, Stefania
Caruso, Enrico
Orlandi, Viviana Teresa
Izzo, Lorella
author_sort Santoro, Orlando
collection PubMed
description In this work, two compounds belonging to the BODIPY family, and previously investigated for their photosensitizing properties, have been bound to the amino-pendant groups of three random copolymers, with different amounts of methyl methacrylate (MMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) in the backbone. The P(MMA-ran-DMAEMA) copolymers have inherently bactericidal activity, due to the amino groups of DMAEMA and to the quaternized nitrogens bounded to BODIPY. Systems consisting of filter paper discs coated with copolymers conjugated to BODIPY were tested on two model microorganisms, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). On solid medium, irradiation with green light induced an antimicrobial effect, visible as a clear inhibition area around the coated disks. The system based on the copolymer with 43% DMAEMA and circa 0.70 wt/wt% of BODIPY was the most efficient in both bacterial species, and a selectivity for the Gram-positive model was observed, independently of the conjugated BODIPY. A residual antimicrobial activity was also observed after dark incubation, attributed to the inherently bactericidal properties of copolymers.
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spelling pubmed-100015702023-03-11 Inherently Antimicrobial P(MMA-ran-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing Santoro, Orlando Malacarne, Miryam Chiara Sarcone, Francesco Scapinello, Luca Pragliola, Stefania Caruso, Enrico Orlandi, Viviana Teresa Izzo, Lorella Int J Mol Sci Communication In this work, two compounds belonging to the BODIPY family, and previously investigated for their photosensitizing properties, have been bound to the amino-pendant groups of three random copolymers, with different amounts of methyl methacrylate (MMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) in the backbone. The P(MMA-ran-DMAEMA) copolymers have inherently bactericidal activity, due to the amino groups of DMAEMA and to the quaternized nitrogens bounded to BODIPY. Systems consisting of filter paper discs coated with copolymers conjugated to BODIPY were tested on two model microorganisms, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). On solid medium, irradiation with green light induced an antimicrobial effect, visible as a clear inhibition area around the coated disks. The system based on the copolymer with 43% DMAEMA and circa 0.70 wt/wt% of BODIPY was the most efficient in both bacterial species, and a selectivity for the Gram-positive model was observed, independently of the conjugated BODIPY. A residual antimicrobial activity was also observed after dark incubation, attributed to the inherently bactericidal properties of copolymers. MDPI 2023-02-22 /pmc/articles/PMC10001570/ /pubmed/36901769 http://dx.doi.org/10.3390/ijms24054340 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Santoro, Orlando
Malacarne, Miryam Chiara
Sarcone, Francesco
Scapinello, Luca
Pragliola, Stefania
Caruso, Enrico
Orlandi, Viviana Teresa
Izzo, Lorella
Inherently Antimicrobial P(MMA-ran-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing
title Inherently Antimicrobial P(MMA-ran-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing
title_full Inherently Antimicrobial P(MMA-ran-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing
title_fullStr Inherently Antimicrobial P(MMA-ran-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing
title_full_unstemmed Inherently Antimicrobial P(MMA-ran-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing
title_short Inherently Antimicrobial P(MMA-ran-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing
title_sort inherently antimicrobial p(mma-ran-dmaema) copolymers sensitive to photodynamic therapy: a double bactericidal effect for active wound dressing
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001570/
https://www.ncbi.nlm.nih.gov/pubmed/36901769
http://dx.doi.org/10.3390/ijms24054340
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