Cargando…
Induction of Angiogenesis by Genetically Modified Human Umbilical Cord Blood Mononuclear Cells
Stimulating the process of angiogenesis in treating ischemia-related diseases is an urgent task for modern medicine, which can be achieved through the use of different cell types. Umbilical cord blood (UCB) continues to be one of the attractive cell sources for transplantation. The goal of this stud...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002409/ https://www.ncbi.nlm.nih.gov/pubmed/36901831 http://dx.doi.org/10.3390/ijms24054396 |
_version_ | 1784904382970593280 |
---|---|
author | Gatina, Dilara Z. Gazizov, Ilnaz M. Zhuravleva, Margarita N. Arkhipova, Svetlana S. Golubenko, Maria A. Gomzikova, Marina O. Garanina, Ekaterina E. Islamov, Rustem R. Rizvanov, Albert A. Salafutdinov, Ilnur I. |
author_facet | Gatina, Dilara Z. Gazizov, Ilnaz M. Zhuravleva, Margarita N. Arkhipova, Svetlana S. Golubenko, Maria A. Gomzikova, Marina O. Garanina, Ekaterina E. Islamov, Rustem R. Rizvanov, Albert A. Salafutdinov, Ilnur I. |
author_sort | Gatina, Dilara Z. |
collection | PubMed |
description | Stimulating the process of angiogenesis in treating ischemia-related diseases is an urgent task for modern medicine, which can be achieved through the use of different cell types. Umbilical cord blood (UCB) continues to be one of the attractive cell sources for transplantation. The goal of this study was to investigate the role and therapeutic potential of gene-engineered umbilical cord blood mononuclear cells (UCB-MC) as a forward-looking strategy for the activation of angiogenesis. Adenovirus constructs Ad-VEGF, Ad-FGF2, Ad-SDF1α, and Ad-EGFP were synthesized and used for cell modification. UCB-MCs were isolated from UCB and transduced with adenoviral vectors. As part of our in vitro experiments, we evaluated the efficiency of transfection, the expression of recombinant genes, and the secretome profile. Later, we applied an in vivo Matrigel plug assay to assess engineered UCB-MC’s angiogenic potential. We conclude that hUCB-MCs can be efficiently modified simultaneously with several adenoviral vectors. Modified UCB-MCs overexpress recombinant genes and proteins. Genetic modification of cells with recombinant adenoviruses does not affect the profile of secreted pro- and anti-inflammatory cytokines, chemokines, and growth factors, except for an increase in the synthesis of recombinant proteins. hUCB-MCs genetically modified with therapeutic genes induced the formation of new vessels. An increase in the expression of endothelial cells marker (CD31) was revealed, which correlated with the data of visual examination and histological analysis. The present study demonstrates that gene-engineered UCB-MC can be used to stimulate angiogenesis and possibly treat cardiovascular disease and diabetic cardiomyopathy. |
format | Online Article Text |
id | pubmed-10002409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100024092023-03-11 Induction of Angiogenesis by Genetically Modified Human Umbilical Cord Blood Mononuclear Cells Gatina, Dilara Z. Gazizov, Ilnaz M. Zhuravleva, Margarita N. Arkhipova, Svetlana S. Golubenko, Maria A. Gomzikova, Marina O. Garanina, Ekaterina E. Islamov, Rustem R. Rizvanov, Albert A. Salafutdinov, Ilnur I. Int J Mol Sci Article Stimulating the process of angiogenesis in treating ischemia-related diseases is an urgent task for modern medicine, which can be achieved through the use of different cell types. Umbilical cord blood (UCB) continues to be one of the attractive cell sources for transplantation. The goal of this study was to investigate the role and therapeutic potential of gene-engineered umbilical cord blood mononuclear cells (UCB-MC) as a forward-looking strategy for the activation of angiogenesis. Adenovirus constructs Ad-VEGF, Ad-FGF2, Ad-SDF1α, and Ad-EGFP were synthesized and used for cell modification. UCB-MCs were isolated from UCB and transduced with adenoviral vectors. As part of our in vitro experiments, we evaluated the efficiency of transfection, the expression of recombinant genes, and the secretome profile. Later, we applied an in vivo Matrigel plug assay to assess engineered UCB-MC’s angiogenic potential. We conclude that hUCB-MCs can be efficiently modified simultaneously with several adenoviral vectors. Modified UCB-MCs overexpress recombinant genes and proteins. Genetic modification of cells with recombinant adenoviruses does not affect the profile of secreted pro- and anti-inflammatory cytokines, chemokines, and growth factors, except for an increase in the synthesis of recombinant proteins. hUCB-MCs genetically modified with therapeutic genes induced the formation of new vessels. An increase in the expression of endothelial cells marker (CD31) was revealed, which correlated with the data of visual examination and histological analysis. The present study demonstrates that gene-engineered UCB-MC can be used to stimulate angiogenesis and possibly treat cardiovascular disease and diabetic cardiomyopathy. MDPI 2023-02-23 /pmc/articles/PMC10002409/ /pubmed/36901831 http://dx.doi.org/10.3390/ijms24054396 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gatina, Dilara Z. Gazizov, Ilnaz M. Zhuravleva, Margarita N. Arkhipova, Svetlana S. Golubenko, Maria A. Gomzikova, Marina O. Garanina, Ekaterina E. Islamov, Rustem R. Rizvanov, Albert A. Salafutdinov, Ilnur I. Induction of Angiogenesis by Genetically Modified Human Umbilical Cord Blood Mononuclear Cells |
title | Induction of Angiogenesis by Genetically Modified Human Umbilical Cord Blood Mononuclear Cells |
title_full | Induction of Angiogenesis by Genetically Modified Human Umbilical Cord Blood Mononuclear Cells |
title_fullStr | Induction of Angiogenesis by Genetically Modified Human Umbilical Cord Blood Mononuclear Cells |
title_full_unstemmed | Induction of Angiogenesis by Genetically Modified Human Umbilical Cord Blood Mononuclear Cells |
title_short | Induction of Angiogenesis by Genetically Modified Human Umbilical Cord Blood Mononuclear Cells |
title_sort | induction of angiogenesis by genetically modified human umbilical cord blood mononuclear cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002409/ https://www.ncbi.nlm.nih.gov/pubmed/36901831 http://dx.doi.org/10.3390/ijms24054396 |
work_keys_str_mv | AT gatinadilaraz inductionofangiogenesisbygeneticallymodifiedhumanumbilicalcordbloodmononuclearcells AT gazizovilnazm inductionofangiogenesisbygeneticallymodifiedhumanumbilicalcordbloodmononuclearcells AT zhuravlevamargaritan inductionofangiogenesisbygeneticallymodifiedhumanumbilicalcordbloodmononuclearcells AT arkhipovasvetlanas inductionofangiogenesisbygeneticallymodifiedhumanumbilicalcordbloodmononuclearcells AT golubenkomariaa inductionofangiogenesisbygeneticallymodifiedhumanumbilicalcordbloodmononuclearcells AT gomzikovamarinao inductionofangiogenesisbygeneticallymodifiedhumanumbilicalcordbloodmononuclearcells AT garaninaekaterinae inductionofangiogenesisbygeneticallymodifiedhumanumbilicalcordbloodmononuclearcells AT islamovrustemr inductionofangiogenesisbygeneticallymodifiedhumanumbilicalcordbloodmononuclearcells AT rizvanovalberta inductionofangiogenesisbygeneticallymodifiedhumanumbilicalcordbloodmononuclearcells AT salafutdinovilnuri inductionofangiogenesisbygeneticallymodifiedhumanumbilicalcordbloodmononuclearcells |