Cargando…

Systematic analysis of myocardial immune progression in septic cardiomyopathy: Immune-related mechanisms in septic cardiomyopathy

BACKGROUND: The immune infiltration and molecular mechanisms underlying septic cardiomyopathy (SC) have not been completely elucidated. This study aimed to identify key genes related to SC and elucidate the potential molecular mechanisms. METHODS: The weighted correlation network analysis (WGCNA), l...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Dunliang, Qin, Xianyu, Zhong, Zhi-an, Liao, Hongtao, Chen, Pengyuan, Zhang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002421/
https://www.ncbi.nlm.nih.gov/pubmed/36911241
http://dx.doi.org/10.3389/fcvm.2022.1036928
_version_ 1784904385933869056
author Ma, Dunliang
Qin, Xianyu
Zhong, Zhi-an
Liao, Hongtao
Chen, Pengyuan
Zhang, Bin
author_facet Ma, Dunliang
Qin, Xianyu
Zhong, Zhi-an
Liao, Hongtao
Chen, Pengyuan
Zhang, Bin
author_sort Ma, Dunliang
collection PubMed
description BACKGROUND: The immune infiltration and molecular mechanisms underlying septic cardiomyopathy (SC) have not been completely elucidated. This study aimed to identify key genes related to SC and elucidate the potential molecular mechanisms. METHODS: The weighted correlation network analysis (WGCNA), linear models for microarray analysis (LIMMA), protein-protein interaction (PPI) network, CIBERSORT, Kyoto Encyclopedia of Genes and Genomes pathway (KEGG), and gene set enrichment analysis (GSEA) were applied to assess the key pathway and hub genes involved in SC. RESULTS: We identified 10 hub genes, namely, LRG1, LCN2, PTX3, E LANE, TCN1, CLEC4D, FPR2, MCEMP1, CEACAM8, and CD177. Furthermore, we used GSEA for all genes and online tools to explore the function of the hub genes. Finally, we took the intersection between differential expression genes (DEGs) and hub genes to identify LCN2 and PTX3 as key genes. We found that immune-related pathways played vital roles in SC. LCN2 and PTX3 were key genes in SC progression, which mainly showed an anti-inflammatory effect. The significant immune cells in cardiomyocytes of SC were neutrophils and M2 macrophages. CONCLUSION: These cells may have the potential to be prognostic and therapeutic targets in the clinical management of SC. Excessive anti-inflammatory function and neutrophil infiltration are probably the primary causes of SC.
format Online
Article
Text
id pubmed-10002421
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-100024212023-03-11 Systematic analysis of myocardial immune progression in septic cardiomyopathy: Immune-related mechanisms in septic cardiomyopathy Ma, Dunliang Qin, Xianyu Zhong, Zhi-an Liao, Hongtao Chen, Pengyuan Zhang, Bin Front Cardiovasc Med Cardiovascular Medicine BACKGROUND: The immune infiltration and molecular mechanisms underlying septic cardiomyopathy (SC) have not been completely elucidated. This study aimed to identify key genes related to SC and elucidate the potential molecular mechanisms. METHODS: The weighted correlation network analysis (WGCNA), linear models for microarray analysis (LIMMA), protein-protein interaction (PPI) network, CIBERSORT, Kyoto Encyclopedia of Genes and Genomes pathway (KEGG), and gene set enrichment analysis (GSEA) were applied to assess the key pathway and hub genes involved in SC. RESULTS: We identified 10 hub genes, namely, LRG1, LCN2, PTX3, E LANE, TCN1, CLEC4D, FPR2, MCEMP1, CEACAM8, and CD177. Furthermore, we used GSEA for all genes and online tools to explore the function of the hub genes. Finally, we took the intersection between differential expression genes (DEGs) and hub genes to identify LCN2 and PTX3 as key genes. We found that immune-related pathways played vital roles in SC. LCN2 and PTX3 were key genes in SC progression, which mainly showed an anti-inflammatory effect. The significant immune cells in cardiomyocytes of SC were neutrophils and M2 macrophages. CONCLUSION: These cells may have the potential to be prognostic and therapeutic targets in the clinical management of SC. Excessive anti-inflammatory function and neutrophil infiltration are probably the primary causes of SC. Frontiers Media S.A. 2023-02-24 /pmc/articles/PMC10002421/ /pubmed/36911241 http://dx.doi.org/10.3389/fcvm.2022.1036928 Text en Copyright © 2023 Ma, Qin, Zhong, Liao, Chen and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Ma, Dunliang
Qin, Xianyu
Zhong, Zhi-an
Liao, Hongtao
Chen, Pengyuan
Zhang, Bin
Systematic analysis of myocardial immune progression in septic cardiomyopathy: Immune-related mechanisms in septic cardiomyopathy
title Systematic analysis of myocardial immune progression in septic cardiomyopathy: Immune-related mechanisms in septic cardiomyopathy
title_full Systematic analysis of myocardial immune progression in septic cardiomyopathy: Immune-related mechanisms in septic cardiomyopathy
title_fullStr Systematic analysis of myocardial immune progression in septic cardiomyopathy: Immune-related mechanisms in septic cardiomyopathy
title_full_unstemmed Systematic analysis of myocardial immune progression in septic cardiomyopathy: Immune-related mechanisms in septic cardiomyopathy
title_short Systematic analysis of myocardial immune progression in septic cardiomyopathy: Immune-related mechanisms in septic cardiomyopathy
title_sort systematic analysis of myocardial immune progression in septic cardiomyopathy: immune-related mechanisms in septic cardiomyopathy
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002421/
https://www.ncbi.nlm.nih.gov/pubmed/36911241
http://dx.doi.org/10.3389/fcvm.2022.1036928
work_keys_str_mv AT madunliang systematicanalysisofmyocardialimmuneprogressioninsepticcardiomyopathyimmunerelatedmechanismsinsepticcardiomyopathy
AT qinxianyu systematicanalysisofmyocardialimmuneprogressioninsepticcardiomyopathyimmunerelatedmechanismsinsepticcardiomyopathy
AT zhongzhian systematicanalysisofmyocardialimmuneprogressioninsepticcardiomyopathyimmunerelatedmechanismsinsepticcardiomyopathy
AT liaohongtao systematicanalysisofmyocardialimmuneprogressioninsepticcardiomyopathyimmunerelatedmechanismsinsepticcardiomyopathy
AT chenpengyuan systematicanalysisofmyocardialimmuneprogressioninsepticcardiomyopathyimmunerelatedmechanismsinsepticcardiomyopathy
AT zhangbin systematicanalysisofmyocardialimmuneprogressioninsepticcardiomyopathyimmunerelatedmechanismsinsepticcardiomyopathy