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Inhibition of γδ-TcR or IL17a Reduces T-Cell and Neutrophil Infiltration after Ischemia/Reperfusion Injury in Mouse Liver

Neutrophil and T-cell recruitment contribute to hepatic ischemia/reperfusion injury. The initial inflammatory response is orchestrated by Kupffer cells and liver sinusoid endothelial cells. However, other cell types, including γδ-Τ cells, seem to be key mediators in further inflammatory cell recruit...

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Autores principales: Al Mogrampi, Saant, Boumpoureka, Christina, Afaloniati, Hara, Lagou, Maria, Angelopoulou, Katerina, Anestakis, Doxakis, Tampouratzi, Zoi Gerasimina, Iliadis, Stavros, Antoniadis, Nikolaos, Giakoustidis, Alexandros, Papalois, Apostolos, Papadopoulos, Vasileios, Poutahidis, Theofilos, Giakoustidis, Dimitrios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002490/
https://www.ncbi.nlm.nih.gov/pubmed/36902538
http://dx.doi.org/10.3390/jcm12051751
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author Al Mogrampi, Saant
Boumpoureka, Christina
Afaloniati, Hara
Lagou, Maria
Angelopoulou, Katerina
Anestakis, Doxakis
Tampouratzi, Zoi Gerasimina
Iliadis, Stavros
Antoniadis, Nikolaos
Giakoustidis, Alexandros
Papalois, Apostolos
Papadopoulos, Vasileios
Poutahidis, Theofilos
Giakoustidis, Dimitrios
author_facet Al Mogrampi, Saant
Boumpoureka, Christina
Afaloniati, Hara
Lagou, Maria
Angelopoulou, Katerina
Anestakis, Doxakis
Tampouratzi, Zoi Gerasimina
Iliadis, Stavros
Antoniadis, Nikolaos
Giakoustidis, Alexandros
Papalois, Apostolos
Papadopoulos, Vasileios
Poutahidis, Theofilos
Giakoustidis, Dimitrios
author_sort Al Mogrampi, Saant
collection PubMed
description Neutrophil and T-cell recruitment contribute to hepatic ischemia/reperfusion injury. The initial inflammatory response is orchestrated by Kupffer cells and liver sinusoid endothelial cells. However, other cell types, including γδ-Τ cells, seem to be key mediators in further inflammatory cell recruitment and proinflammatory cytokine release, including IL17a. In this study, we used an in vivo model of partial hepatic ischemia/reperfusion injury (IRI) to investigate the role of the γδ-Τ-cell receptor (γδTcR) and the role of IL17a in the pathogenesis of liver injury. Forty C57BL6 mice were subjected to 60 min of ischemia followed by 6 h of reperfusion (RN 6339/2/2016). Pretreatment with either anti-γδΤcR antibodies or anti-IL17a antibodies resulted in a reduction in histological and biochemical markers of liver injury as well as neutrophil and T-cell infiltration, inflammatory cytokine production and the downregulation of c-Jun and NF-κΒ. Overall, neutralizing either γδTcR or IL17a seems to have a protective role in liver IRI.
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spelling pubmed-100024902023-03-11 Inhibition of γδ-TcR or IL17a Reduces T-Cell and Neutrophil Infiltration after Ischemia/Reperfusion Injury in Mouse Liver Al Mogrampi, Saant Boumpoureka, Christina Afaloniati, Hara Lagou, Maria Angelopoulou, Katerina Anestakis, Doxakis Tampouratzi, Zoi Gerasimina Iliadis, Stavros Antoniadis, Nikolaos Giakoustidis, Alexandros Papalois, Apostolos Papadopoulos, Vasileios Poutahidis, Theofilos Giakoustidis, Dimitrios J Clin Med Article Neutrophil and T-cell recruitment contribute to hepatic ischemia/reperfusion injury. The initial inflammatory response is orchestrated by Kupffer cells and liver sinusoid endothelial cells. However, other cell types, including γδ-Τ cells, seem to be key mediators in further inflammatory cell recruitment and proinflammatory cytokine release, including IL17a. In this study, we used an in vivo model of partial hepatic ischemia/reperfusion injury (IRI) to investigate the role of the γδ-Τ-cell receptor (γδTcR) and the role of IL17a in the pathogenesis of liver injury. Forty C57BL6 mice were subjected to 60 min of ischemia followed by 6 h of reperfusion (RN 6339/2/2016). Pretreatment with either anti-γδΤcR antibodies or anti-IL17a antibodies resulted in a reduction in histological and biochemical markers of liver injury as well as neutrophil and T-cell infiltration, inflammatory cytokine production and the downregulation of c-Jun and NF-κΒ. Overall, neutralizing either γδTcR or IL17a seems to have a protective role in liver IRI. MDPI 2023-02-22 /pmc/articles/PMC10002490/ /pubmed/36902538 http://dx.doi.org/10.3390/jcm12051751 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al Mogrampi, Saant
Boumpoureka, Christina
Afaloniati, Hara
Lagou, Maria
Angelopoulou, Katerina
Anestakis, Doxakis
Tampouratzi, Zoi Gerasimina
Iliadis, Stavros
Antoniadis, Nikolaos
Giakoustidis, Alexandros
Papalois, Apostolos
Papadopoulos, Vasileios
Poutahidis, Theofilos
Giakoustidis, Dimitrios
Inhibition of γδ-TcR or IL17a Reduces T-Cell and Neutrophil Infiltration after Ischemia/Reperfusion Injury in Mouse Liver
title Inhibition of γδ-TcR or IL17a Reduces T-Cell and Neutrophil Infiltration after Ischemia/Reperfusion Injury in Mouse Liver
title_full Inhibition of γδ-TcR or IL17a Reduces T-Cell and Neutrophil Infiltration after Ischemia/Reperfusion Injury in Mouse Liver
title_fullStr Inhibition of γδ-TcR or IL17a Reduces T-Cell and Neutrophil Infiltration after Ischemia/Reperfusion Injury in Mouse Liver
title_full_unstemmed Inhibition of γδ-TcR or IL17a Reduces T-Cell and Neutrophil Infiltration after Ischemia/Reperfusion Injury in Mouse Liver
title_short Inhibition of γδ-TcR or IL17a Reduces T-Cell and Neutrophil Infiltration after Ischemia/Reperfusion Injury in Mouse Liver
title_sort inhibition of γδ-tcr or il17a reduces t-cell and neutrophil infiltration after ischemia/reperfusion injury in mouse liver
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002490/
https://www.ncbi.nlm.nih.gov/pubmed/36902538
http://dx.doi.org/10.3390/jcm12051751
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