Cargando…

SWATH-MS-Based Proteomics Reveals the Regulatory Metabolism of Amaryllidaceae Alkaloids in Three Lycoris Species

Alkaloids are a class of nitrogen-containing alkaline organic compounds found in nature, with significant biological activity, and are also important active ingredients in Chinese herbal medicine. Amaryllidaceae plants are rich in alkaloids, among which galanthamine, lycorine, and lycoramine are rep...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Meng, Li, Chaohan, Zhang, Cheng, Cai, Youming, Zhang, Yongchun, Yang, Liuyan, Chen, Moxian, Zhu, Fuyuan, Li, Qingzhu, Li, Kehu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002500/
https://www.ncbi.nlm.nih.gov/pubmed/36901927
http://dx.doi.org/10.3390/ijms24054495
_version_ 1784904402956451840
author Tang, Meng
Li, Chaohan
Zhang, Cheng
Cai, Youming
Zhang, Yongchun
Yang, Liuyan
Chen, Moxian
Zhu, Fuyuan
Li, Qingzhu
Li, Kehu
author_facet Tang, Meng
Li, Chaohan
Zhang, Cheng
Cai, Youming
Zhang, Yongchun
Yang, Liuyan
Chen, Moxian
Zhu, Fuyuan
Li, Qingzhu
Li, Kehu
author_sort Tang, Meng
collection PubMed
description Alkaloids are a class of nitrogen-containing alkaline organic compounds found in nature, with significant biological activity, and are also important active ingredients in Chinese herbal medicine. Amaryllidaceae plants are rich in alkaloids, among which galanthamine, lycorine, and lycoramine are representative. Since the difficulty and high cost of synthesizing alkaloids have been the major obstacles in industrial production, particularly the molecular mechanism underlying alkaloid biosynthesis is largely unknown. Here, we determined the alkaloid content in Lycoris longituba, Lycoris incarnata, and Lycoris sprengeri, and performed a SWATH-MS (sequential window acquisition of all theoretical mass spectra)-based quantitative approach to detect proteome changes in the three Lycoris. A total of 2193 proteins were quantified, of which 720 proteins showed a difference in abundance between Ll and Ls, and 463 proteins showed a difference in abundance between Li and Ls. KEGG enrichment analysis revealed that differentially expressed proteins are distributed in specific biological processes including amino acid metabolism, starch, and sucrose metabolism, implicating a supportive role for Amaryllidaceae alkaloids metabolism in Lycoris. Furthermore, several key genes collectively known as OMT and NMT were identified, which are probably responsible for galanthamine biosynthesis. Interestingly, RNA processing-related proteins were also abundantly detected in alkaloid-rich Ll, suggesting that posttranscriptional regulation such as alternative splicing may contribute to the biosynthesis of Amaryllidaceae alkaloids. Taken together, our SWATH-MS-based proteomic investigation may reveal the differences in alkaloid contents at the protein levels, providing a comprehensive proteome reference for the regulatory metabolism of Amaryllidaceae alkaloids.
format Online
Article
Text
id pubmed-10002500
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100025002023-03-11 SWATH-MS-Based Proteomics Reveals the Regulatory Metabolism of Amaryllidaceae Alkaloids in Three Lycoris Species Tang, Meng Li, Chaohan Zhang, Cheng Cai, Youming Zhang, Yongchun Yang, Liuyan Chen, Moxian Zhu, Fuyuan Li, Qingzhu Li, Kehu Int J Mol Sci Article Alkaloids are a class of nitrogen-containing alkaline organic compounds found in nature, with significant biological activity, and are also important active ingredients in Chinese herbal medicine. Amaryllidaceae plants are rich in alkaloids, among which galanthamine, lycorine, and lycoramine are representative. Since the difficulty and high cost of synthesizing alkaloids have been the major obstacles in industrial production, particularly the molecular mechanism underlying alkaloid biosynthesis is largely unknown. Here, we determined the alkaloid content in Lycoris longituba, Lycoris incarnata, and Lycoris sprengeri, and performed a SWATH-MS (sequential window acquisition of all theoretical mass spectra)-based quantitative approach to detect proteome changes in the three Lycoris. A total of 2193 proteins were quantified, of which 720 proteins showed a difference in abundance between Ll and Ls, and 463 proteins showed a difference in abundance between Li and Ls. KEGG enrichment analysis revealed that differentially expressed proteins are distributed in specific biological processes including amino acid metabolism, starch, and sucrose metabolism, implicating a supportive role for Amaryllidaceae alkaloids metabolism in Lycoris. Furthermore, several key genes collectively known as OMT and NMT were identified, which are probably responsible for galanthamine biosynthesis. Interestingly, RNA processing-related proteins were also abundantly detected in alkaloid-rich Ll, suggesting that posttranscriptional regulation such as alternative splicing may contribute to the biosynthesis of Amaryllidaceae alkaloids. Taken together, our SWATH-MS-based proteomic investigation may reveal the differences in alkaloid contents at the protein levels, providing a comprehensive proteome reference for the regulatory metabolism of Amaryllidaceae alkaloids. MDPI 2023-02-24 /pmc/articles/PMC10002500/ /pubmed/36901927 http://dx.doi.org/10.3390/ijms24054495 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Meng
Li, Chaohan
Zhang, Cheng
Cai, Youming
Zhang, Yongchun
Yang, Liuyan
Chen, Moxian
Zhu, Fuyuan
Li, Qingzhu
Li, Kehu
SWATH-MS-Based Proteomics Reveals the Regulatory Metabolism of Amaryllidaceae Alkaloids in Three Lycoris Species
title SWATH-MS-Based Proteomics Reveals the Regulatory Metabolism of Amaryllidaceae Alkaloids in Three Lycoris Species
title_full SWATH-MS-Based Proteomics Reveals the Regulatory Metabolism of Amaryllidaceae Alkaloids in Three Lycoris Species
title_fullStr SWATH-MS-Based Proteomics Reveals the Regulatory Metabolism of Amaryllidaceae Alkaloids in Three Lycoris Species
title_full_unstemmed SWATH-MS-Based Proteomics Reveals the Regulatory Metabolism of Amaryllidaceae Alkaloids in Three Lycoris Species
title_short SWATH-MS-Based Proteomics Reveals the Regulatory Metabolism of Amaryllidaceae Alkaloids in Three Lycoris Species
title_sort swath-ms-based proteomics reveals the regulatory metabolism of amaryllidaceae alkaloids in three lycoris species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002500/
https://www.ncbi.nlm.nih.gov/pubmed/36901927
http://dx.doi.org/10.3390/ijms24054495
work_keys_str_mv AT tangmeng swathmsbasedproteomicsrevealstheregulatorymetabolismofamaryllidaceaealkaloidsinthreelycorisspecies
AT lichaohan swathmsbasedproteomicsrevealstheregulatorymetabolismofamaryllidaceaealkaloidsinthreelycorisspecies
AT zhangcheng swathmsbasedproteomicsrevealstheregulatorymetabolismofamaryllidaceaealkaloidsinthreelycorisspecies
AT caiyouming swathmsbasedproteomicsrevealstheregulatorymetabolismofamaryllidaceaealkaloidsinthreelycorisspecies
AT zhangyongchun swathmsbasedproteomicsrevealstheregulatorymetabolismofamaryllidaceaealkaloidsinthreelycorisspecies
AT yangliuyan swathmsbasedproteomicsrevealstheregulatorymetabolismofamaryllidaceaealkaloidsinthreelycorisspecies
AT chenmoxian swathmsbasedproteomicsrevealstheregulatorymetabolismofamaryllidaceaealkaloidsinthreelycorisspecies
AT zhufuyuan swathmsbasedproteomicsrevealstheregulatorymetabolismofamaryllidaceaealkaloidsinthreelycorisspecies
AT liqingzhu swathmsbasedproteomicsrevealstheregulatorymetabolismofamaryllidaceaealkaloidsinthreelycorisspecies
AT likehu swathmsbasedproteomicsrevealstheregulatorymetabolismofamaryllidaceaealkaloidsinthreelycorisspecies