Antimicrobial Activity of Sertraline on Listeria monocytogenes

We explored the antimicrobial activity of sertraline on Listeria monocytogenes and further investigated the effects of sertraline on biofilm formation and the virulence gene expression of L. monocytogenes. The minimum inhibitory concentration and minimum bactericidal concentration for sertraline aga...

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Autores principales: Wang, Yidi, Li, Lili, Cai, Pingyao, Olsen, Rikke Heidemann, Peng, Shuai, Meng, Hecheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002541/
https://www.ncbi.nlm.nih.gov/pubmed/36902108
http://dx.doi.org/10.3390/ijms24054678
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author Wang, Yidi
Li, Lili
Cai, Pingyao
Olsen, Rikke Heidemann
Peng, Shuai
Meng, Hecheng
author_facet Wang, Yidi
Li, Lili
Cai, Pingyao
Olsen, Rikke Heidemann
Peng, Shuai
Meng, Hecheng
author_sort Wang, Yidi
collection PubMed
description We explored the antimicrobial activity of sertraline on Listeria monocytogenes and further investigated the effects of sertraline on biofilm formation and the virulence gene expression of L. monocytogenes. The minimum inhibitory concentration and minimum bactericidal concentration for sertraline against L. monocytogenes were in the range of 16–32 μg/mL and 64 μg/mL, respectively. Sertraline-dependent damage of the cell membrane and a decrease in intracellular ATP and pH(in) in L. monocytogenes were observed. In addition, sertraline reduced the biofilm formation efficiency of the L. monocytogenes strains. Importantly, low concentrations (0.1 μg/mL and 1 μg/mL) of sertraline significantly down-regulated the expression levels of various L. monocytogens virulence genes (prfA, actA, degU, flaA, sigB, ltrC and sufS). These results collectively suggest a role of sertraline for the control of L. monocytogenes in the food industry.
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spelling pubmed-100025412023-03-11 Antimicrobial Activity of Sertraline on Listeria monocytogenes Wang, Yidi Li, Lili Cai, Pingyao Olsen, Rikke Heidemann Peng, Shuai Meng, Hecheng Int J Mol Sci Article We explored the antimicrobial activity of sertraline on Listeria monocytogenes and further investigated the effects of sertraline on biofilm formation and the virulence gene expression of L. monocytogenes. The minimum inhibitory concentration and minimum bactericidal concentration for sertraline against L. monocytogenes were in the range of 16–32 μg/mL and 64 μg/mL, respectively. Sertraline-dependent damage of the cell membrane and a decrease in intracellular ATP and pH(in) in L. monocytogenes were observed. In addition, sertraline reduced the biofilm formation efficiency of the L. monocytogenes strains. Importantly, low concentrations (0.1 μg/mL and 1 μg/mL) of sertraline significantly down-regulated the expression levels of various L. monocytogens virulence genes (prfA, actA, degU, flaA, sigB, ltrC and sufS). These results collectively suggest a role of sertraline for the control of L. monocytogenes in the food industry. MDPI 2023-02-28 /pmc/articles/PMC10002541/ /pubmed/36902108 http://dx.doi.org/10.3390/ijms24054678 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yidi
Li, Lili
Cai, Pingyao
Olsen, Rikke Heidemann
Peng, Shuai
Meng, Hecheng
Antimicrobial Activity of Sertraline on Listeria monocytogenes
title Antimicrobial Activity of Sertraline on Listeria monocytogenes
title_full Antimicrobial Activity of Sertraline on Listeria monocytogenes
title_fullStr Antimicrobial Activity of Sertraline on Listeria monocytogenes
title_full_unstemmed Antimicrobial Activity of Sertraline on Listeria monocytogenes
title_short Antimicrobial Activity of Sertraline on Listeria monocytogenes
title_sort antimicrobial activity of sertraline on listeria monocytogenes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002541/
https://www.ncbi.nlm.nih.gov/pubmed/36902108
http://dx.doi.org/10.3390/ijms24054678
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AT lilili antimicrobialactivityofsertralineonlisteriamonocytogenes
AT caipingyao antimicrobialactivityofsertralineonlisteriamonocytogenes
AT olsenrikkeheidemann antimicrobialactivityofsertralineonlisteriamonocytogenes
AT pengshuai antimicrobialactivityofsertralineonlisteriamonocytogenes
AT menghecheng antimicrobialactivityofsertralineonlisteriamonocytogenes

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