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FRET assay for live-cell high-throughput screening of the cardiac SERCA pump yields multiple classes of small-molecule allosteric modulators

We have used FRET-based biosensors in live cells, in a robust high-throughput screening (HTS) platform, to identify small-molecules that alter the structure and activity of the cardiac sarco/endoplasmic reticulum calcium ATPase (SERCA2a). Our primary aim is to discover drug-like small-molecule activ...

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Detalles Bibliográficos
Autores principales:	Roopnarine, Osha, Yuen, Samantha L., Thompson, Andrew R., Roelike, Lauren N., Rebbeck, Robyn T., Bidwell, Phillip A., Aldrich, Courtney C., Cornea, Razvan L., Thomas, David D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Journal Experts 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002828/ https://www.ncbi.nlm.nih.gov/pubmed/36909610 http://dx.doi.org/10.21203/rs.3.rs-2596384/v1

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