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Temperature modulates the osmosensitivity of tilapia prolactin cells

In euryhaline fish, prolactin (Prl) plays an essential role in freshwater (FW) acclimation. In the euryhaline and eurythermal Mozambique tilapia, Oreochromis mossambicus, Prl cells are model osmoreceptors, recently described to be thermosensitive. To investigate the effects of temperature on osmorec...

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Autores principales: Hewage, Tharindu Malintha Gardi, Woo, Daniel W., Celino-Brady, Fritzle T., Seale, Andre P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002831/
https://www.ncbi.nlm.nih.gov/pubmed/36909603
http://dx.doi.org/10.21203/rs.3.rs-2524830/v1
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author Hewage, Tharindu Malintha Gardi
Woo, Daniel W.
Celino-Brady, Fritzle T.
Seale, Andre P.
author_facet Hewage, Tharindu Malintha Gardi
Woo, Daniel W.
Celino-Brady, Fritzle T.
Seale, Andre P.
author_sort Hewage, Tharindu Malintha Gardi
collection PubMed
description In euryhaline fish, prolactin (Prl) plays an essential role in freshwater (FW) acclimation. In the euryhaline and eurythermal Mozambique tilapia, Oreochromis mossambicus, Prl cells are model osmoreceptors, recently described to be thermosensitive. To investigate the effects of temperature on osmoreception, we incubated Prl cells of tilapia acclimated to either FW or seawater (SW) in different temperature (20, 26 and 32°C) and osmolality (280, 330 and 420 mOsm/kg) combinations for 6 h. Release of both Prl isoforms, Prl(188) and Prl(177), increased in hyposmotic media and were further augmented with a rise in temperature. Hyposmotically-induced release of Prl(188) was inhibited at 20°C. In SW fish, mRNA expression of prl(188) and prl(177) showed direct and inverse relationships with temperature, respectively. In SW-acclimated tilapia Prl cells incubated in hyperosmotic media, Prl receptors, prlr1 and prlr2, and the stretch-activated Ca(2+) channel, trpv4, were inhibited at 32°C, suggesting the presence of a cellular mechanism to compensate for elevated Prl release. Transcription factors, pou1f1, pou2f1b, creb3l1, cebpb, stat3, stat1a and nfat1c, known to regulate prl(188) and prl(177), were also downregulated at 32°C. Our findings provide evidence that osmoreception is modulated by temperature, and that both thermal and osmotic responses vary with acclimation salinity.
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spelling pubmed-100028312023-03-11 Temperature modulates the osmosensitivity of tilapia prolactin cells Hewage, Tharindu Malintha Gardi Woo, Daniel W. Celino-Brady, Fritzle T. Seale, Andre P. Res Sq Article In euryhaline fish, prolactin (Prl) plays an essential role in freshwater (FW) acclimation. In the euryhaline and eurythermal Mozambique tilapia, Oreochromis mossambicus, Prl cells are model osmoreceptors, recently described to be thermosensitive. To investigate the effects of temperature on osmoreception, we incubated Prl cells of tilapia acclimated to either FW or seawater (SW) in different temperature (20, 26 and 32°C) and osmolality (280, 330 and 420 mOsm/kg) combinations for 6 h. Release of both Prl isoforms, Prl(188) and Prl(177), increased in hyposmotic media and were further augmented with a rise in temperature. Hyposmotically-induced release of Prl(188) was inhibited at 20°C. In SW fish, mRNA expression of prl(188) and prl(177) showed direct and inverse relationships with temperature, respectively. In SW-acclimated tilapia Prl cells incubated in hyperosmotic media, Prl receptors, prlr1 and prlr2, and the stretch-activated Ca(2+) channel, trpv4, were inhibited at 32°C, suggesting the presence of a cellular mechanism to compensate for elevated Prl release. Transcription factors, pou1f1, pou2f1b, creb3l1, cebpb, stat3, stat1a and nfat1c, known to regulate prl(188) and prl(177), were also downregulated at 32°C. Our findings provide evidence that osmoreception is modulated by temperature, and that both thermal and osmotic responses vary with acclimation salinity. American Journal Experts 2023-02-28 /pmc/articles/PMC10002831/ /pubmed/36909603 http://dx.doi.org/10.21203/rs.3.rs-2524830/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Hewage, Tharindu Malintha Gardi
Woo, Daniel W.
Celino-Brady, Fritzle T.
Seale, Andre P.
Temperature modulates the osmosensitivity of tilapia prolactin cells
title Temperature modulates the osmosensitivity of tilapia prolactin cells
title_full Temperature modulates the osmosensitivity of tilapia prolactin cells
title_fullStr Temperature modulates the osmosensitivity of tilapia prolactin cells
title_full_unstemmed Temperature modulates the osmosensitivity of tilapia prolactin cells
title_short Temperature modulates the osmosensitivity of tilapia prolactin cells
title_sort temperature modulates the osmosensitivity of tilapia prolactin cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002831/
https://www.ncbi.nlm.nih.gov/pubmed/36909603
http://dx.doi.org/10.21203/rs.3.rs-2524830/v1
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