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Diagnostic Performance of Immunohistochemistry Compared to Molecular Techniques for Microsatellite Instability and p53 Mutation Detection in Endometrial Cancer

Molecular algorithms may estimate the risk of recurrence and death for patients with endometrial cancer (EC) and may impact treatment decisions. To detect microsatellite instabilities (MSI) and p53 mutations, immunohistochemistry (IHC) and molecular techniques are used. To select the most appropriat...

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Autores principales: Streel, Sylvie, Salmon, Alixe, Dheur, Adriane, Bours, Vincent, Leroi, Natacha, Habran, Lionel, Delbecque, Katty, Goffin, Frédéric, Pleyers, Clémence, Kakkos, Athanasios, Gonne, Elodie, Seidel, Laurence, Kridelka, Frédéric, Gennigens, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002995/
https://www.ncbi.nlm.nih.gov/pubmed/36902292
http://dx.doi.org/10.3390/ijms24054866
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author Streel, Sylvie
Salmon, Alixe
Dheur, Adriane
Bours, Vincent
Leroi, Natacha
Habran, Lionel
Delbecque, Katty
Goffin, Frédéric
Pleyers, Clémence
Kakkos, Athanasios
Gonne, Elodie
Seidel, Laurence
Kridelka, Frédéric
Gennigens, Christine
author_facet Streel, Sylvie
Salmon, Alixe
Dheur, Adriane
Bours, Vincent
Leroi, Natacha
Habran, Lionel
Delbecque, Katty
Goffin, Frédéric
Pleyers, Clémence
Kakkos, Athanasios
Gonne, Elodie
Seidel, Laurence
Kridelka, Frédéric
Gennigens, Christine
author_sort Streel, Sylvie
collection PubMed
description Molecular algorithms may estimate the risk of recurrence and death for patients with endometrial cancer (EC) and may impact treatment decisions. To detect microsatellite instabilities (MSI) and p53 mutations, immunohistochemistry (IHC) and molecular techniques are used. To select the most appropriate method, and to have an accurate interpretation of their results, knowledge of the performance characteristics of these respective methods is essential. The objective of this study was to assess the diagnostic performance of IHC versus molecular techniques (gold standard). One hundred and thirty-two unselected EC patients were enrolled in this study. Agreement between the two diagnostic methods was assessed using Cohen’s kappa coefficient. Sensitivity, specificity, positive (PPV) and negative predictive values (NPV) of the IHC were calculated. For MSI status, the sensitivity, specificity, PPV and NPV were 89.3%, 87.3%, 78.1% and 94.1%, respectively. Cohen’s kappa coefficient was 0.74. For p53 status, the sensitivity, specificity, PPV, and NPV were 92.3%, 77.1%, 60.0% and 96.4%, respectively. Cohen’s kappa coefficient was 0.59. For MSI status, IHC presented a substantial agreement with the polymerase chain reaction (PCR) approach. For the p53 status, the moderate agreement observed between IHC and next generation sequencing (NGS) methods implies that they cannot be used interchangeably.
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spelling pubmed-100029952023-03-11 Diagnostic Performance of Immunohistochemistry Compared to Molecular Techniques for Microsatellite Instability and p53 Mutation Detection in Endometrial Cancer Streel, Sylvie Salmon, Alixe Dheur, Adriane Bours, Vincent Leroi, Natacha Habran, Lionel Delbecque, Katty Goffin, Frédéric Pleyers, Clémence Kakkos, Athanasios Gonne, Elodie Seidel, Laurence Kridelka, Frédéric Gennigens, Christine Int J Mol Sci Article Molecular algorithms may estimate the risk of recurrence and death for patients with endometrial cancer (EC) and may impact treatment decisions. To detect microsatellite instabilities (MSI) and p53 mutations, immunohistochemistry (IHC) and molecular techniques are used. To select the most appropriate method, and to have an accurate interpretation of their results, knowledge of the performance characteristics of these respective methods is essential. The objective of this study was to assess the diagnostic performance of IHC versus molecular techniques (gold standard). One hundred and thirty-two unselected EC patients were enrolled in this study. Agreement between the two diagnostic methods was assessed using Cohen’s kappa coefficient. Sensitivity, specificity, positive (PPV) and negative predictive values (NPV) of the IHC were calculated. For MSI status, the sensitivity, specificity, PPV and NPV were 89.3%, 87.3%, 78.1% and 94.1%, respectively. Cohen’s kappa coefficient was 0.74. For p53 status, the sensitivity, specificity, PPV, and NPV were 92.3%, 77.1%, 60.0% and 96.4%, respectively. Cohen’s kappa coefficient was 0.59. For MSI status, IHC presented a substantial agreement with the polymerase chain reaction (PCR) approach. For the p53 status, the moderate agreement observed between IHC and next generation sequencing (NGS) methods implies that they cannot be used interchangeably. MDPI 2023-03-02 /pmc/articles/PMC10002995/ /pubmed/36902292 http://dx.doi.org/10.3390/ijms24054866 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Streel, Sylvie
Salmon, Alixe
Dheur, Adriane
Bours, Vincent
Leroi, Natacha
Habran, Lionel
Delbecque, Katty
Goffin, Frédéric
Pleyers, Clémence
Kakkos, Athanasios
Gonne, Elodie
Seidel, Laurence
Kridelka, Frédéric
Gennigens, Christine
Diagnostic Performance of Immunohistochemistry Compared to Molecular Techniques for Microsatellite Instability and p53 Mutation Detection in Endometrial Cancer
title Diagnostic Performance of Immunohistochemistry Compared to Molecular Techniques for Microsatellite Instability and p53 Mutation Detection in Endometrial Cancer
title_full Diagnostic Performance of Immunohistochemistry Compared to Molecular Techniques for Microsatellite Instability and p53 Mutation Detection in Endometrial Cancer
title_fullStr Diagnostic Performance of Immunohistochemistry Compared to Molecular Techniques for Microsatellite Instability and p53 Mutation Detection in Endometrial Cancer
title_full_unstemmed Diagnostic Performance of Immunohistochemistry Compared to Molecular Techniques for Microsatellite Instability and p53 Mutation Detection in Endometrial Cancer
title_short Diagnostic Performance of Immunohistochemistry Compared to Molecular Techniques for Microsatellite Instability and p53 Mutation Detection in Endometrial Cancer
title_sort diagnostic performance of immunohistochemistry compared to molecular techniques for microsatellite instability and p53 mutation detection in endometrial cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10002995/
https://www.ncbi.nlm.nih.gov/pubmed/36902292
http://dx.doi.org/10.3390/ijms24054866
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